NFATC1/NFAT2 Antibody

Images

 
Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Analysis of NFATc1 in Ramos cell lysate. Lane 1. Without blocking peptide. Lane 2. With blocking peptide.
Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody [NB100-56732] - NFAT2 antibody was tested in Raw 246.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Analysis of human Ramos (A) and mouse RAW (B) lysate using NFATc1 antibody at 1 ug/ml.
Simple Western: NFATC1/NFAT2 Antibody [NB100-56732] - Simple Western lane view shows a specific band for NFAT2 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NFATC1/NFAT2 Antibody Summary

Immunogen
Amino acids 264-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 were used as immunogen for this antibody.
Specificity
The amino acid sequence used is 90% homologous in mouse and rat and 100% identical in human NFATc1 A, B, C and E isoforms. The reported amino acid size of NFATc1 isoforms are as follows: A -716 aa, B-825 aa, C-930 aa, and E-812 aa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFATC1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:20
Application Notes
Immunoreative bands can be blocked by preincubation of NB100-56732 with immunizing peptide. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon testing material.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
NFATC1/NFAT2 Lysate (NBL1-13608)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56732 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NFATC1/NFAT2 Antibody

  • MGC138448
  • NFAT transcription complex cytosolic component
  • NFAT2
  • NFAT2cytoplasmic 1
  • NFATC
  • NF-ATC
  • NFATC1
  • NF-ATc1
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

Background

NFAT (nuclear factor of activated T cell) transcription factors play critical roles in gene transcription during immune responses. The NFAT complex is made up of NFATc (NFATc1 - 4) and AP-1 transcription factor. NFAT proteins are regulated by Ca2+. NFATc1 is a substrate protein phosphatase calcineurin, a critical mediator of calcium signals during Tcell activation. NFATc1 activation requires the translocation of the transcription factor into the nucleus, a process that is mediated by calcineurin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for NFATC1/NFAT2 Antibody (NB100-56732) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56732:
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Images Ratings Applications Species Date Details
Western Blot NFATC1/NFAT2 NB100-56732
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reviewed by:
Anonymous
WB Mouse 02/12/2016
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Summary

ApplicationWestern Blot
SpeciesMouse
FileView PDF

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Secondary Antibodies

 

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Anonymous
02/12/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol NFATC1
Entrez
Uniprot