Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Immortalized mouse podocytes. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody [NB100-56732] - NFAT2 antibody was tested in Raw 246.7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Analysis of human (A)Ramos and (B) mouse RAW lysate using NFATc1 antibody at 1 ug/ml.
Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Analysis of NFATc1 in Ramos cell lysate. (1) Without blocking peptide. (2) With blocking peptide.
Simple Western: NFATC1/NFAT2 Antibody [NB100-56732] - Lane view shows a specific band for NFAT2 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Amino acids 264-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 were used as immunogen for this antibody.
The amino acid sequence used is 90% homologous in mouse and rat and 100% identical in human NFATc1 A, B, C and E isoforms. The reported amino acid size of NFATc1 isoforms are as follows: A -716 aa, B-825 aa, C-930 aa, and E-812 aa.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in IHC reported in scientific literature (PMID:35167499) Immunoreative bands can be blocked by preincubation of NB100-56732 with immunizing peptide. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon testing material.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue..
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for NFATC1/NFAT2 Antibody
NFAT transcription complex cytosolic component
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
NFAT (nuclear factor of activated T cell) transcription factors play critical roles in gene transcription during immune responses. The NFAT complex is made up of NFATc (NFATc1 - 4) and AP-1 transcription factor. NFAT proteins are regulated by Ca2+. NFATc1 is a substrate protein phosphatase calcineurin, a critical mediator of calcium signals during Tcell activation. NFATc1 activation requires the translocation of the transcription factor into the nucleus, a process that is mediated by calcineurin.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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