MCAF1 Antibody

Images

 
Genetic Strategies: Western Blot: MCAF1 Antibody [NBP1-84610] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ATF7IP antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: MCAF1 Antibody [NBP1-84610] - Staining of human cell line A-431 shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human testis shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining in human testis and skeletal muscle tissues using anti-ATF7IP antibody. Corresponding ATF7IP RNA-seq data are presented ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Analysis in human testis and skeletal muscle tissues. Corresponding MCAF1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human skeletal muscle shows low nuclear positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MCAF1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSP
Specificity
Specificity of human MCAF1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATF7IP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MCAF1 Protein (NBP1-84610PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-84610 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MCAF1 Antibody

  • activating transcription factor 7 interacting protein
  • activating transcription factor 7-interacting protein 1
  • ATF7-interacting protein
  • ATFa-associated modulator
  • ATF-interacting protein
  • ATF-IP
  • FLJ10139
  • FLJ10688
  • hAM
  • MBD1-containing chromatin associated factor
  • MCAF1AM
  • MCAFMBD1-containing chromatin-associated factor 1
  • MMCP 6
  • MMCP6
  • MMCP-6
  • p621

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCAF1 Antibody (NBP1-84610) (0)

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Review for MCAF1 Antibody (NBP1-84610) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-84610:
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WB
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Images Ratings Applications Species Date Details
Western Blot MCAF1 NBP1-84610
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4
reviewed by:
Wei-Ting Lu
WB Human 01/18/2019
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Summary

ApplicationWestern Blot
Sample TestedH1944 cell line
SpeciesHuman
Lotr09412

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MCAF1 Antibody (NBP1-84610) (0)

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Secondary Antibodies

 

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Additional MCAF1 Products

Array NBP1-84610

Bioinformatics Tool for MCAF1 Antibody (NBP1-84610)

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Diseases for MCAF1 Antibody (NBP1-84610)

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Learn the difference between western blot and simple western

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Wei-Ting Lu
01/18/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATF7IP