CDKN3 Antibody (OTI2E11)

Images

 
Western Blot: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: CDKN3 Antibody (2E11) [NBP2-01245] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN3.
Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human prostate tissue using anti-CDKN3 mouse monoclonal antibody.
Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed ...read more
Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CDKN3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human endometrium tissue using anti-CDKN3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CDKN3 Antibody (2E11) [NBP2-01245] - Staining of paraffin-embedded Human Kidney tissue using anti-CDKN3 mouse monoclonal antibody.
Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - Analysis of Jurkat cells, using anti-CDKN3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI2E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.56 mg/ml

Order Details

CDKN3 Antibody (OTI2E11) Summary

Immunogen
Full length human recombinant protein of human CDKN3(NP_005183) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDKN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
23.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.56 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDKN3 Antibody (OTI2E11)

  • CDI1KAP1
  • CDK2-associated dual specificity phosphatase
  • CDK2-associated dual-specificity phosphatase
  • Cdk-associated protein phosphatase
  • CIP2
  • cyclin-dependent kinase inhibitor 3
  • cyclin-dependent kinase interacting protein 2
  • Cyclin-dependent kinase interactor 1
  • Cyclin-dependent kinase-interacting protein 2
  • EC 3.1.3.16
  • EC 3.1.3.48
  • FLJ25787
  • KAPMGC70625
  • Kinase-associated phosphatase

Background

The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDKN3 Antibody (NBP2-01245) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70399AF350
Alexa Fluor 405 NBP2-70399AF405
Alexa Fluor 488 NBP2-70399AF488
Alexa Fluor 532 NBP2-70399AF532
Alexa Fluor 594 NBP2-70399AF594
Alexa Fluor 647 NBP2-70399AF647
Alexa Fluor 700 NBP2-70399AF700
Alexa Fluor 750 NBP2-70399AF750
Allophycocyanin NBP2-70399APC
Allophycocyanin/Cy7 NBP2-70399APCCY7
Biotin NBP2-70399B
DyLight 350 NBP2-70399UV
DyLight 405 NBP2-70399V
DyLight 488 NBP2-70399G
DyLight 550 NBP2-70399R
DyLight 594 NBP2-70399DL594
DyLight 650 NBP2-70399C
DyLight 680 NBP2-70399FR
DyLight 755 NBP2-70399IR
FITC NBP2-70399F
HRP NBP2-70399H
Janelia Fluor 549 NBP2-70399JF549
Janelia Fluor 646 NBP2-70399JF646
PE NBP2-70399PE
PE/Atto594 NBP2-70399PEATT594
PE/Cy5.5 NBP2-70399PECY55
PE/Cy7 NBP2-70399PECY7
PerCP NBP2-70399PCP

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Bioinformatics

Gene Symbol CDKN3
Entrez