SETDB1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody [NBP1-84790] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm.
Immunohistochemistry-Paraffin: SETDB1 Antibody [NBP1-84790] - Staining of human colon shows strong nuclear, cytoplasmic and membranous positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SETDB1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:KLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDISCRDFIEEYVTAYPNRPMVLLKSGQLIKTEWEGTWWKSRVEEVDGSLVRILFLDDKRCEWIYRGSTRLEPMFSMKTSSASALEKKQGQLRTRPNMGAVRSKGPVVQYTQD
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SETDB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
SETDB1 Protein (NBP1-84790PEP)
Publications
Read Publications using NBP1-84790.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23770855)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SETDB1 Antibody

  • ERG-associated protein with SET domain
  • ESETEC 2.1.1.43
  • H3-K9-HMTase 4
  • Histone H3-K9 methyltransferase 4
  • histone-lysine N-methyltransferase SETDB1
  • histone-lysine N-methyltransferase, H3lysine-9 specific 4
  • KG1T
  • KIAA0067H3-K9-HMTase4
  • KMT1EERG-associated protein with a SET domain, ESET
  • Lysine N-methyltransferase 1E
  • SET domain bifurcated 1
  • SET domain, bifurcated 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SETDB1 Antibody (NBP1-84790)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SETDB1 Antibody (NBP1-84790)

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Bioinformatics

Gene Symbol SETDB1
Entrez
Uniprot