Mouse Neutrophil Elastase/ELA2 DuoSet ELISA, 5 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Neutrophil Elastase/ELA2 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Elane

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Neutrophil Elastase/ELA2 DuoSet ELISA, 5 Plate

• Bone marrow serine protease
• EC 3.4.21
• EC 3.4.21.37
• ELA2
• ELA2granulocyte-derived elastase
• ELANE
• elastase 2, neutrophil
• elastase, neutrophil expressed
• Elastase-2
• GE
• HLEelastase-2
• HNE
• Human leukocyte elastase
• Leukocyte Elastase
• Medullasin
• NE
• Neutrophil Elastase
• PMN elastase
• PMN-E
• polymorphonuclear elastase
• SCN1

Background

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Publications for Neutrophil Elastase/ELA2 (DY4517-05)(17)

Publications using DY4517-05

• Park, JS;Lee, J;Wang, F;Ma, H;Zhou, Z;Lee, YS;Oh, K;Lee, H;Sui, G;Lee, S;Yang, YM;Lee, JW;Ji, YH;Park, CW;Yoo, HS;Hwang, BY;Han, SB;Song, N;Oh, S;Kim, B;Seki, E;Hong, JT;Roh, YS; A1AT dysregulation of metabolically stressed hepatocytes by Kupffer cells drives MASH and fibrosis Experimental & molecular medicine 2025-02-01 [PMID: 39939782] (Human)
• Das, S;Kaminski, TW;Schlegel, BT;Bain, W;Hu, S;Patel, A;Kale, SL;Chen, K;Lee, JS;Mallampalli, RK;Kagan, VE;Rajasundaram, D;McVerry, BJ;Sundd, P;Kitsios, GD;Ray, A;Ray, P; Neutrophils and galectin-3 defend mice from lethal bacterial infection and humans from acute respiratory failure Nature communications 2024-06-03 [PMID: 38830855] (Mouse)
• M Costa Fuji, A Yazdanpana, S Horne, A Lamont, P Lopez, C Farr Zuend, K Birse, M Taverner, R Greenslade, M Abou, L Noel-Romas, B Abrenica, O Ajibola, N Ikeogu, RC Su, LR McKinnon, H Pymar, V Poliquin, AR Berard, A Burgener, TT Murooka Non-optimal bacteria species induce neutrophil-driven inflammation and barrier disruption in the female genital tract Mucosal Immunology, 2023-04-29;0(0):. 2023-04-29 [PMID: 37121385] (ELISA, Mouse)
• J Zhao, N Zhen, Q Zhou, J Lou, W Cui, G Zhang, B Tian NETs Promote Inflammatory Injury by Activating cGAS-STING Pathway in Acute Lung Injury International Journal of Molecular Sciences, 2023-03-07;24(6):. 2023-03-07 [PMID: 36982193] (Mouse)
• P Kumar, A Mittal, P Bansal, S Aggarwal, RK Rajnish, A Dadra, S Sharma Wound alpha defensin levels are significantly higher in patients with fracture related infection: A pilot, prospective cohort study Injury, 2022-12-13;0(0):. 2022-12-13 [PMID: 36567156] (Human)
• RA Keogh, AL Haeberle, CJ Langouët-A, JS Kavanaugh, EP Schmidt, GD Moore, AR Horswill, KS Doran Group B Streptococcus adaptation promotes survival in a hyperinflammatory diabetic wound environment Science Advances, 2022-11-11;8(45):eadd3221. 2022-11-11 [PMID: 36367946] (Mouse, Transgenic Mouse)
• A Gupta, K Singh, S Fatima, S Ambreen, S Zimmermann, R Younis, S Krishnan, R Rana, I Gadi, C Schwab, R Biemann, K Shahzad, V Rani, S Ali, PR Mertens, S Kohli, B Isermann Neutrophil Extracellular Traps Promote NLRP3 Inflammasome Activation and Glomerular Endothelial Dysfunction in Diabetic Kidney Disease Nutrients, 2022-07-20;14(14):. 2022-07-20 [PMID: 35889923] (Mouse)
• Xintong Dong, Nathachit Limjunyawong, Elizabeth I. Sypek, Gaofeng Wang, Roger V. Ortines, Christine Youn et al. Keratinocyte-derived defensins activate neutrophil-specific receptors Mrgpra2a/b to prevent skin dysbiosis and bacterial infection Immunity 2022-09-01 [PMID: 35882236] (Mouse)
• KC Ngamsri, RA Putri, C Jans, K Schindler, A Fuhr, Y Zhang, J Gamper-Tsi, S Ehnert, FM Konrad CXCR4 and CXCR7 Inhibition Ameliorates the Formation of Platelet-Neutrophil Complexes and Neutrophil Extracellular Traps through Adora2b Signaling International Journal of Molecular Sciences, 2021-12-17;22(24):. 2021-12-17 [PMID: 34948374] (Mouse)
• P Saha, BS Yeoh, X Xiao, RM Golonka, V Singh, Y Wang, M Vijay-Kuma PAD4-dependent NETs generation are indispensable for intestinal clearance of Citrobacter rodentium Mucosal Immunol, 2019-02-01;0(0):. 2019-02-01 [PMID: 30710097] (Mouse)
• S Aggarwal, I Ahmad, A Lam, MA Carlisle, C Li, JM Wells, SV Raju, M Athar, SM Rowe, MT Dransfield, S Matalon Heme scavenging reduces pulmonary endoplasmic reticulum stress, fibrosis, and emphysema JCI Insight, 2018-11-02;3(21):. 2018-11-02 [PMID: 30385726] (Mouse)
• S Al-Salam, S Hashmi Myocardial Ischemia Reperfusion Injury: Apoptotic, Inflammatory and Oxidative Stress Role of Galectin-3 Cell. Physiol. Biochem., 2018-10-24;50(3):1123-1139. 2018-10-24 [PMID: 30355930] (Mouse)
• P Ochieng, S Nath, R Macarulay, E Eden, A Dabo, M Campos, XC Jiang, RF Foronjy, P Geraghty Phospholipid transfer protein and alpha-1 antitrypsin regulate Hck kinase activity during neutrophil degranulation Sci Rep, 2018-10-18;8(1):15394. 2018-10-18 [PMID: 30337619] (Mouse)
• X Xiao, P Saha, BS Yeoh, JA Hipp, V Singh, M Vijay-Kuma Myeloperoxidase deficiency attenuates systemic and dietary iron-induced adverse effects J. Nutr. Biochem., 2018-08-21;62(0):28-34. 2018-08-21 [PMID: 30218980] (Mouse)
• G Wen, W An, J Chen, EM Maguire, Q Chen, F Yang, SWA Pearce, M Kyriakides, L Zhang, S Ye, S Nourshargh, Q Xiao Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis J Am Heart Assoc, 2018-02-08;7(4):. 2018-02-08 [PMID: 29437605] (Mouse)
• A Bao, KF Che, S Bozinovski, J Ji, JA Gregory, S Kumlien Ge, M Adner, LO Cardell, A Lindén Recombinant human IL-26 facilitates the innate immune response to endotoxin in the bronchoalveolar space of mice in vivo PLoS ONE, 2017-12-05;12(12):e0188909. 2017-12-05 [PMID: 29206862] (Mouse)
• XS Ren, L Ling, B Zhou, Y Han, YB Zhou, Q Chen, YH Li, YM Kang, GQ Zhu Silencing salusin-? attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats Sci Rep, 2017-02-23;7(0):43259. 2017-02-23 [PMID: 28230187] (Rat)

FAQs for Neutrophil Elastase/ELA2 (DY4517-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Elane