Serpin A1a Antibody (882213) [Unconjugated]

Western blot shows mouse serum. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Mouse Serpin A1a Monoclonal Antibody (Catalog # MAB7690) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Serpin A1a at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows mouse serum, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1a at approximately 62 kDa (as indicated) using 2 µg/mL of Rat Anti-Mouse Serpin A1a Monoclonal Antibody (Catalog # MAB7690) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western
• Clone: 882213
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin A1a Antibody (882213) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Serpin A1a
  Met1-Lys413
  Accession # P07758
• Specificity: Detects mouse Serpin A1a in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) Serpin A1c, approximately 15% cross-reactivity with recombinant human A1, and less than 5% cross-reactivity with rmSerpin A3n is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Serpina1a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A1a Antibody (882213) [Unconjugated]

• A1A
• A1AT
• AAT
• Aat2
• alpha1AT
• Dom1
• nNIF
• PI
• PI1
• PRO2275
• Serpin A1a
• Spi1-1

Background

Serpin A1a, also known as alpha-1-antitrypsin, belongs to the serpin superfamily of proteinase inhibitors. It is one of five alpha 1-proteinase inhibitors produced in mice. Serpin A1a is produced in the liver, then secreted into the bloodstream. It inhibits a number of serine proteases, including elastase, trypsin, plasmin, and thrombin (1, 2). Serpin A1a’s major function is to protect the lungs from neutrophil elastase. Serpin A1a deficiency can cause complications such as emphysema, and chronic obstructive pulmonary disease (COPD).

1. Gettins, P.G. (2002) Chem. Rev. 102:4751.
2. Kalsheker N.A. (1989) Biosci. Rep. 9:129. 3. Ritchie, R.F. et al. (2000) J. Clin. Lab. Anal. 14:284.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A1a Antibody (MAB7690)(2)

Publications using MAB7690

• D Pakalnišky, T Schönberge, B Strobel, B Stierstorf, T Lamla, M Schuler, M Lenter Rosa26-LSL-dCas9-VPR: a versatile mouse model for tissue specific and simultaneous activation of multiple genes for drug discovery Scientific Reports, 2022-11-10;12(1):19268. 2022-11-10 [PMID: 36357523] (Simple Western, Transgenic Mouse)
• Higgins NR, Greenslade JE, Wu JJ et al. Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD Brain pathology (Zurich, Switzerland) 3/29/2021 [PMID: 33780087]

Bioinformatics

• Gene Symbol: Serpina1a
• Uniprot:
  • P07758(Mouse)