Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin ...read more
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Albumin depleted human serum. Lane 2: none. Load: 10 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody ...read more
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for ...read more
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target ...read more
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of ...read more
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
a1-Anti-Trypsin [Human Plasma]
alpha 1 Antitrypsin
Ion exchange chromatography
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Sodium Phosphate (pH 7.2) and 0.15M NaCl
0.01% Sodium Azide
Ion exchange chromatography
Reconstitute with 0.1 ml deionized water.
Western Blot 1:2000 - 1:10000
ELISA 1:20000 - 1:100000
Immunoprecipitation 1:10 - 1:500
This product has been assayed against 1.0 ug of a_1-Anti-Trypsin [Human Plasma] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. Immunoprecipitation was reported in scientific literature.
Alternate Names for Serpin A1/alpha 1-Antitrypsin Antibody
alpha 1-Proteinase Inhibitor
Alpha-1 protease inhibitor
antitrypsin), member 1
serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
serine (or cysteine) proteinase inhibitor, clade A, member 1
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
Alpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.