Serpin A1/alpha 1-Antitrypsin Antibody [HRP]

Images

 
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [HRP] [NB120-7635] - Lane 1: molecular weight. Lane 2: Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST. Load: 35 ug per lane under ...read more
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [HRP] [NB120-7635] - Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP

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Catalog# & Conjugate Size Price

Serpin A1/alpha 1-Antitrypsin Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
a1-Anti-Trypsin [Human Plasma]
Localization
Secreted
Specificity
alpha 1 Antitrypsin
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SERPINA1
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:4000-1:20000
  • Immunohistochemistry 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:500-1:2500
Positive Control
Serpin A8/Angiotensinogen Lysate (NBL1-07391)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Purity
Delipidation and Defibrination

Alternate Names for Serpin A1/alpha 1-Antitrypsin Antibody [HRP]

  • A1A
  • A1AT
  • AATMGC23330
  • alpha 1-Antitrypsin
  • alpha 1-Proteinase Inhibitor
  • Alpha-1 protease inhibitor
  • Alpha-1-antiproteinase
  • alpha-1-antitrypsin
  • antitrypsin), member 1
  • member 1
  • PIMGC9222
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • serine (or cysteine) proteinase inhibitor, clade A, member 1
  • Serpin A1
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SERPINA1
Entrez