Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY alpha 1 Antitrypsin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-al

Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - Staining of paraffin-embedded Human pancreas tissue using anti-alpha 1 Antitrypsin mouse monoclonal antibody.

Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-alpha 1 Antitrypsin antibody, and then analyzed by flow cytometry.

Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-alpha 1 Antitrypsin mouse monoclonal antibody.

Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-alpha 1 Antitrypsin mouse monoclonal antibody.

Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-alpha 1 Antitrypsin mouse monoclonal antibody.

Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - Staining of paraffin-embedded Human liver tissue using anti-alpha 1 Antitrypsin mouse monoclonal antibody.

Immunoprecipitation: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - Immunoprecipitation (IP) of SERPINA1 by using a monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SERPINA1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Reactivity: Hu, Ca, Pm, Pm
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clone: OTI9A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free Summary

• Immunogen: Full-length protein expressed in 293T cell transfected with human SERPINA1 expression vector
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:500-1000
• Theoretical MW: 46.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free

• A1A
• A1AT
• AATMGC23330
• alpha 1-Antitrypsin
• alpha 1-Proteinase Inhibitor
• Alpha-1 protease inhibitor
• Alpha-1-antiproteinase
• alpha-1-antitrypsin
• antitrypsin), member 1
• member 1
• PIMGC9222
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• serine (or cysteine) proteinase inhibitor, clade A, member 1
• Serpin A1
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Alpha 1 Antitrypsin, also known as SERPINA1, is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINA1