Serpin A1/alpha 1-Antitrypsin Antibody (2B12)

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (2B12) [NBP1-47444] - Analysis using AAT mouse mAb against human plasma (1) and NIH/3T3 cell lysate (2).

• Reactivity: Hu, Mu
• Applications: WB, DB, ELISA
• Clone: 2B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Serpin A1/alpha 1-Antitrypsin Antibody (2B12) Summary

• Immunogen: Purified recombinant fragment of human Serpin A1/alpha 1-Antitrypsin expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINA1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100 - 1:2000
  • ELISA 1:10000
  • Western Blot 1:500 - 1:2000
• Application Notes: Dot Blot reactivity reported in scientific literature (PMID: 23028890).
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Ascites

Alternate Names for Serpin A1/alpha 1-Antitrypsin Antibody (2B12)

• A1A
• A1AT
• AATMGC23330
• alpha 1-Antitrypsin
• alpha 1-Proteinase Inhibitor
• Alpha-1 protease inhibitor
• Alpha-1-antiproteinase
• alpha-1-antitrypsin
• antitrypsin), member 1
• member 1
• PIMGC9222
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• serine (or cysteine) proteinase inhibitor, clade A, member 1
• Serpin A1
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A1/alpha 1-Antitrypsin Antibody (NBP1-47444)(1)

Publication using NBP1-47444

• Hoopes SL, Willcockson HH, Caron KM. Characteristics of Multi-Organ Lymphangiectasia Resulting from Temporal Deletion of Calcitonin Receptor-Like Receptor in Adult Mice PLoS One 2012-01-01 [PMID: 23028890] (Cytometric Bead Assay Standard, Mouse)

Bioinformatics

• Gene Symbol: SERPINA1
• Entrez:
  • 5265(Human)
• Uniprot:
  • P01009()