SENP1 Antibody

Images

 
Western Blot: SENP1 Antibody [NB100-56405] - analysis of SENP1 in A) Daudi, B) HeLa and C) NIH 3T3 cell lysate using this antibody.
Simple Western: SENP1 Antibody [NB100-56405] - Simple Western lane view shows a specific band for SENP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SENP1 Antibody Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to aa 578-590 of human SENP1.
Specificity
A 66 kDa band is observed. In human and mouse, SENP1 has different isoforms between 664 and 699 amino acids long. Additional bands seen in western blot may represent other SUMOlated proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SENP1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 3 - 5 ug/ml
  • Simple Western 1:50
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Positive Control
Jurkat Lysate (NB800-PC2)
NIH 3T3 Lysate (NB800-PC8)
Publications
Read Publications using
NB100-56405 in the following applications:

Reactivity Notes

Reacts with Mouse and Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for SENP1 Antibody

  • EC 3.4.22
  • EC 3.4.22.-
  • SENP1
  • Sentrin/SUMO-specific protease SENP1
  • sentrin-specific protease 1
  • SUMO1/sentrin specific peptidase 1
  • SUMO1/sentrin specific protease 1
  • SuPr-2

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SENP1 Antibody (NB100-56405)(3)

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SENP1
Entrez