SENP1 Antibody

Western Blot: SENP1 Antibody [NB100-56405] - analysis of SENP1 in A) Daudi, B) HeLa and C) NIH 3T3 cell lysate using this antibody.

Simple Western: SENP1 Antibody [NB100-56405] - Simple Western lane view shows a specific band for SENP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SENP1 Antibody Summary

• Immunogen: This antibody was developed against a synthetic peptide corresponding to aa 578-590 of human SENP1.
• Specificity: A 66 kDa band is observed. In human and mouse, SENP1 has different isoforms between 664 and 699 amino acids long. Additional bands seen in western blot may represent other SUMOlated proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SENP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 3 - 5 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. Use in Immunocytochemistry/immunofluorescence, and Immunohistochemistry-paraffin reported in scientific literature (PMID: 27882949).
• Control:

  Jurkat Whole Cell Lysate
  NIH 3T3 Whole Cell Lysate

Reactivity Notes

Reacts with Mouse and Human.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for SENP1 Antibody

• EC 3.4.22
• EC 3.4.22.-
• SENP1
• Sentrin/SUMO-specific protease SENP1
• sentrin-specific protease 1
• SUMO1/sentrin specific peptidase 1
• SUMO1/sentrin specific protease 1
• SuPr-2

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SENP1 Antibody (NB100-56405)(3)

Publications using NB100-56405

• Zhang H, Wang Y, Zhu A et al. SUMO-specific protease 1 protects neurons from apoptotic death during transient brain ischemia/reperfusion. Cell Death Dis. Nov 24 2016 [PMID: 27882949] (IHC-P, ICC/IF, WB, Mouse)
• La Salle S, Sun F, Zhang XD et al. Developmental control of sumoylation pathway proteins in mouse male germ cells. Dev Biol. 2008 Sep 1 [PMID: 18602382] (WB)
  
  Details:
  SAE2 (IMG-5111A), PIASg (IMG-290), SENP1 CT (IMG-521). WB: Male germ cells; leptotene/zygotene, prepubertal pachytene, and adult pachytene spermatocytes, Fig 5.
• Park J, Seo T, Kim H, Choe J. Sumoylation of the novel protein hRIP{beta} is involved in replication protein A deposition in PML nuclear bodies. Mol Cell Biol. 2005 Sep [PMID: 16135809]

Bioinformatics

• Gene Symbol: SENP1
• Entrez:
  • 223870(Mouse)