SENP5 Antibody

Western Blot: SENP5 Antibody [NB100-56412] - Lysate from human HeLa cells probed with this antibody at 1:250.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SENP5 Antibody Summary

• Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids 229-246 (CNRKPFINREITNYRARH) of human SENP5
• Specificity: The antibody should recognize SENP5 isoforms containing the immunogen sequence (CNRKPFINREITNYRARH) used to make the antibody. Isoform sizes from 163-755 amino acids have been described for SENP5. Researchers are encouraged to BLAST the sequence (NCBI.NIH.GOV) to gain additional information.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SENP5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000
• Application Notes: A 50 kDa band is observed. Molecular weights observed may vary, depending isoform(s) present in the lysate.
• Control:

  Jurkat Whole Cell Lysate
  NIH 3T3 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SENP5 Antibody

• DKFZp564O1016
• EC 3.4.22
• EC 3.4.22.-
• FLJ42398
• MGC27076
• Sentrin/SUMO-specific protease SENP5
• sentrin-specific protease 5
• SUMO1/sentrin specific peptidase 5
• SUMO1/sentrin specific protease 5

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SENP5
• Entrez:
  • 320213(Mouse)
• Uniprot:
  • Q96HI0(Human)