PIASy Antibody

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Western Blot: PIASy Antibody [NB600-1318] - Western blot analysis of PIASg in Jurkat cell lysate using NB600-1318 at 2 ug/ml.
Western Blot: PIASy Antibody [NB600-1318] - Analysis of PIASin A) human liver, B) mouse liver, C) rat liver and D) human Jurkat lysate using this antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PIASy Antibody Summary

Immunogen
This polyclonal antibody was developed against a mixture of synthetic peptides corresponding to amino acids 46-60 (KKIKELYETRYAKKNC) and 187-198 (YSDTSCPQEDQY) of human PIASg; GenBank no. AAD45155.1|AF164437_1. These sequences are 95% and 100% homologous in mouse and rat.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIAS4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 3 - 5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Wester Blot: Detects a band of approximately 55 kDa. Immunocytochemistry/Immunofluorescence: please see Ihara et al, 2005 for details.
Reviewed Applications
Read 1 Review rated 3
using
NB600-1318 in the following applications:

Publications
Read Publications using
NB600-1318 in the following applications:

  • 5 publications
  • WB
    3 publications

Reactivity Notes

Cross-reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for PIASy Antibody

  • E3 SUMO-protein ligase PIAS4
  • FLJ12419
  • MGC35296
  • Piasg
  • PIAS-gamma
  • PIASY
  • Protein inhibitor of activated STAT protein 4
  • Protein inhibitor of activated STAT protein gamma
  • protein inhibitor of activated STAT protein PIASy
  • protein inhibitor of activated STAT, 4
  • zinc finger, MIZ-type containing 6
  • ZMIZ6

Background

The interaction of a cytokine with its specific cell surface receptor triggers the activation of intracellular signaling pathways that ultimately program the cellular response. The signal transducers and activators of transcription (STAT) proteins play important roles in the activation of these pathways. Recently, a family of proteins has been identified that inhibits activated STATS and are named as Protein Inhibitors of Activated STAT (PIAS). PIAS proteins comprise a family of five mammalian proteins, which have been identified as potentially important down regulators of the STAT-signaling pathway. The cDNA for mouse and human PIASg encodes a protein of 507 amino acids that is highly homologous to the human protein and is expressed in the mouse as early as d 7.5 of gestation. In situ hybridizations of staged mouse embryos localized the transcript for the PIASg gene to the limbs, neuroepithelium, and the inner root sheath of the hair follicle, suggesting a role in the development of these structures. This protein is also present in hair follicle of human scalp hair and in monkey neuronal cells. Thus PIASg exhibits a highly selective pattern of expression, suggesting that it modulates the response of cells to developmental cues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Supplier Logo

Publications for PIASy Antibody (NB600-1318)(6)

We have publications tested in 2 confirmed species: Human, uman.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(5)
WB
(3)
All Applications
Filter By Species
Human
(3)
uman
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB600-1318 Applications Species
Tong Hua, Shen Rong, Wang ZhuMing et al. DNA ploidy cytometry testing for cervical cancer screening in China (DNACIC Trial): a prospective randomized, controlled trial. Clin Cancer Res. 2009 Oct 15 [PMID: 19825960] (ICC/IF, Human) ICC/IF Human
La Salle S, Sun F, Zhang XD et al. Developmental control of sumoylation pathway proteins in mouse male germ cells. Dev Biol. 2008 Sep 1 [PMID: 18602382] (WB)

Details:
SAE2 (IMG-5111A), PIASg (IMG-290), SENP1 CT (IMG-521). WB: Male germ cells; leptotene/zygotene, prepubertal pachytene, and adult pachytene spermatocytes, Fig 5.
WB
Brayman MJ, Dharmaraj N, Lagow E et al. MUC1 expression is repressed by protein inhibitor of activated signal transducer and activator of transcription-y. Mol Endocrinol. 2007 Nov [PMID: 17717071] (ICC/IF, Human)

Details:
IF/ICC (confocal microscopy): Fig 8B, F [HEC-1A cells stably transfected with human progesterone receptor B (PRB) and left untreated or treated with progesterone].
ICC/IF Human
Albor A, El-Hizawi S, Horn EJ et al. The interaction of Piasy with Trim32, an E3-ubiquitin ligase mutated in limb-girdle muscular dystrophy type 2H, promotes Piasy degradation and regulates UVB-induced keratinocyte apoptosis through NFkappaB. J Biol Chem. 2006 Sep 1 [PMID: 16816390] (ICC/IF, uman)

Details:
IF/ICC (confocal microscopy): Fig 5 (primary human dermal fibroblasts).
ICC/IF uman
Ihara M, Yamamoto H, Kikuchi A. SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy-dependent activation of Tcf-4. Mol Cell Biol. 2005 May [PMID: 15831457] (WB, ICC/IF)

Details:
WB: Fg 1F (HeLa cell lysates immunoprecipitatated with His6-SUMO-1). IF/ICC (confocal micrcoscopy): Fig 3Aa (COS cells).
WB, ICC/IF
Zoumpoulidou G, Jones MC, Fernandez de Mattos S et al. Convergence of interferon-gamma and progesterone signaling pathways in human endometrium: role of PIASy (protein inhibitor of activated signal transducer and activator of transcription-y). Mol Endocrinol. 2004 Aug [PMID: 15155784] (WB, ICC/IF, Human)

Details:
WB: Fig 5A [primary human endometrial stromal cells (ESCs)]; IF/ICC (confocal microscopy): Fig 6C (PIASy or progesterone transfected ESCs).
WB, ICC/IF Human

Review for PIASy Antibody (NB600-1318) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-1318:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PIASy NB600-1318
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reviewed by:
Anonymous
WB Human 10/02/2014
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Summary

ApplicationWestern Blot
Sample TestedJurkat cells
SpeciesHuman
CommentsThe band of interest is very weak even after long exposure. There are a few other bands. But this antibody is marginally better

Blocking

Blocking DetailsBSA (5%) in PBST

Primary Anitbody

Dilution Ratio1:1K in PBST buffer

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit IgG Antibody (HRP)-Novus
Secondary Manufacturer Cat#Cat No NB7160
Secondary ConcentrationDiluted 20,000 times

Details

Detection NotesDetected using PromoKine ECL Chemiluminescence Detection Kit with a 2 min exposure on X-ray film

Comments

CommentsThe band of interest is very weak even after long exposure. There are a few other bands. But this antibody is marginally better

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PIASy Antibody (NB600-1318) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for PIASy Antibody (NB600-1318)

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Recent Reviews

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5
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1
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Anonymous
10/02/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PIAS4
Entrez
Uniprot