UBE2I/Ubc9 Antibody

Images

 
Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Human breast cancer tissue. IHC-P image submitted by a verified customer review.
Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney. Antibody at 0.3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Product Details

Summary
Reactivity Hu, Rt, Mu, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

UBE2I/Ubc9 Antibody Summary

Immunogen
Peptide with sequence SGIALSRLAQERK-C corresponding to N-Terminus according to NP_919235.1, NP_919236.1, NP_919237.1.
Specificity
This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
Predicted Species
Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UBE2I
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/mL
  • Immunohistochemistry 1 ug/mL
  • Immunohistochemistry-Paraffin 1 ug/mL
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 17 kDa band observed in human kidney, lung and testis lysates and in mouse and rat heart, spleen and testis lysates (calculated MW of 18.0 kDa band according to human NP_003336.1). IHC-P: Human kidney show nuclear staining of some epithelial cells of glomeruli and renal tubules.
Control
UBE2I/Ubc9 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB300-812 in the following applications:

Publications
Read Publications using
NB300-812 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBE2I/Ubc9 Antibody

  • C358B7.1
  • EC 6.3.2
  • EC 6.3.2.-
  • p18
  • SUMO-1-protein ligase
  • SUMO-protein Ligase
  • Ubc9
  • UBC9SUMO-conjugating enzyme UBC9
  • UBCE9
  • UBE2I
  • Ubiquitin carrier protein 9
  • Ubiquitin carrier protein I
  • ubiquitin conjugating enzyme 9
  • Ubiquitin-conjugating enzyme E2 I
  • ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • ubiquitin-conjugating enzyme UbcE2A
  • ubiquitin-like protein SUMO-1 conjugating enzyme
  • ubiquitin-protein ligase E2I
  • Ubiquitin-protein ligase I

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for UBE2I/Ubc9 Antibody (NB300-812)(3)

Reviews for UBE2I/Ubc9 Antibody (NB300-812) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB300-812:
Filter by Applications
IHC-P
(1)
WB
(2)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin UBE2I/Ubc9 NB300-812
Enlarge
reviewed by:
Anonymous
IHC-P Human 05/03/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested breast cancer tissue
SpeciesHuman
Lotcan not find lot No. on my vial
Western Blot UBE2I/Ubc9 NB300-812
Enlarge
reviewed by:
Anonymous
WB Human 12/27/2019
View

Summary

ApplicationWestern Blot
Sample Tested Hela whole cell lysate
SpeciesHuman
Lotcan not find the lot number on the original tube
Western Blot UBE2I/Ubc9 NB300-812
Enlarge
reviewed by:
Anonymous
WB Human 10/28/2018
View

Summary

ApplicationWestern Blot
Sample TestedRCC-1,SN12C
SpeciesHuman
LotNo lot number printed on the tube

Product General Protocols

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Video Protocols

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FAQs for UBE2I/Ubc9 Antibody (NB300-812) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics Tool for UBE2I/Ubc9 Antibody (NB300-812)

Discover related pathways, diseases and genes to UBE2I/Ubc9 Antibody (NB300-812). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Anonymous
05/03/2020
Application: IHC-P
Species: Human

Anonymous
12/27/2019
Application: WB
Species: Human

Anonymous
10/28/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol UBE2I
Entrez
Uniprot