UBE2I/Ubc9 Antibody

Images

 
Western Blot: UBE2I Antibody [NB300-812] - Analysis of UBE2I in Human Kidney lysate (35ug protein in RIPA buffer) using NB300-812 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: UBE2I Antibody [NB300-812] - (0.3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

UBE2I/Ubc9 Antibody Summary

Immunogen
Peptide with sequence SGIALSRLAQERK-C corresponding to N-Terminus according to NP_919235.1, NP_919236.1, NP_919237.1.
Specificity
This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UBE2I
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 ug/ml
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
ICC: An anonymous customer has reported positive results in mouse oocytes. WB: Approx. 17 kDa band observed in human kidney, lung and testis lysates and in mouse and rat heart, spleen and testis lysates (calculated MW of 18.0 kDa band according to human NP_003336.1). IHC-P: Human kidney show nuclear staining of some epithelial cells of glomeruli and renal tubules.
Positive Control
UBE2I/Ubc9 Lysate (NBL1-17532)
Publications
Read Publications using NB300-812.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBE2I/Ubc9 Antibody

  • C358B7.1
  • EC 6.3.2
  • EC 6.3.2.-
  • p18
  • SUMO-1-protein ligase
  • SUMO-protein Ligase
  • Ubc9
  • UBC9SUMO-conjugating enzyme UBC9
  • UBCE9
  • UBE2I
  • Ubiquitin carrier protein 9
  • Ubiquitin carrier protein I
  • ubiquitin conjugating enzyme 9
  • Ubiquitin-conjugating enzyme E2 I
  • ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • ubiquitin-conjugating enzyme UbcE2A
  • ubiquitin-like protein SUMO-1 conjugating enzyme
  • ubiquitin-protein ligase E2I
  • Ubiquitin-protein ligase I

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UBE2I/Ubc9 Antibody (NB300-812)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBE2I
Entrez
Uniprot