UBE2I/Ubc9 Antibody

Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Human breast cancer tissue. IHC-P image submitted by a verified customer review.

Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney. Antibody at 0.3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

• Reactivity: Hu, Rt, Mu, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UBE2I/Ubc9 Antibody Summary

• Immunogen: Peptide with sequence SGIALSRLAQERK-C corresponding to N-Terminus according to NP_919235.1, NP_919236.1, NP_919237.1.
• Specificity: This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UBE2I
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1 ug/mL
  • Immunohistochemistry-Paraffin 1 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: WB: Approx. 17 kDa band observed in human kidney, lung and testis lysates and in mouse and rat heart, spleen and testis lysates (calculated MW of 18.0 kDa band according to human NP_003336.1). IHC-P: Human kidney show nuclear staining of some epithelial cells of glomeruli and renal tubules.
• Control:

  UBE2I/Ubc9 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBE2I/Ubc9 Antibody

• C358B7.1
• EC 6.3.2
• EC 6.3.2.-
• p18
• SUMO-1-protein ligase
• SUMO-protein Ligase
• Ubc9
• UBC9SUMO-conjugating enzyme UBC9
• UBCE9
• UBE2I
• Ubiquitin carrier protein 9
• Ubiquitin carrier protein I
• ubiquitin conjugating enzyme 9
• Ubiquitin-conjugating enzyme E2 I
• ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
• ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• ubiquitin-conjugating enzyme UbcE2A
• ubiquitin-like protein SUMO-1 conjugating enzyme
• ubiquitin-protein ligase E2I
• Ubiquitin-protein ligase I

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBE2I/Ubc9 Antibody (NB300-812)(3)

Publications using NB300-812

• Rousseaux MW, Revelli JP, Vazquez-Velez GE et al. Depleting Trim28 in adult mice is well tolerated and reduces levels of alpha-synuclein and tau Elife 2018-06-04 [PMID: 29863470] (WB, Human)
• Uemura A, Taniguchi M, Matsuo Y et al. UBC9 regulates the stability of XBP1, a key transcription factor controlling the ER stress response. Cell Struct Funct. 2013-01-01 [PMID: 23470653]
• Wang ZY, Qiu QQ, Seufert W et al. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J Biol Chem 1996-10-04 [PMID: 8798754]

Reviews for UBE2I/Ubc9 Antibody (NB300-812) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Lei Nie IHC-P Human 05/03/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Breast cancer tissue
• Species: Human
• Lot: can not find lot No. on my vial

reviewed by: Xianghou Xia WB Human 12/27/2019

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: can not find the lot number on the original tube

reviewed by: Junwei HOU WB Human 10/28/2018

Summary

• Application: Western Blot
• Sample Tested: RCC-1,SN12C
• Species: Human
• Lot: No lot number printed on the tube

Bioinformatics

• Gene Symbol: UBE2I
• Entrez:
  • 7329(Human)
  • 22196(Mouse)
  • 25573(Rat)
• Uniprot:
  • NP_003336.(Human)