SUMO1 Antibody (SM1/495)

Images

 
Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.
Immunohistochemistry-Paraffin: SUMO1 Antibody (SM1/495) [NBP2-32901] - Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-1 MAb (SM1/495)
SDS-Page: SUMO1 Antibody (SM1/495) [NBP2-32901]

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, PAGE
Clone
SM1/495
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

SUMO1 Antibody (SM1/495) Summary

Immunogen
Recombinant human SUMO1 protein
Localization
Predominantly nuclear with some cytoplasmic
Specificity
This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SUMO1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • SDS-Page
Application Notes
ELISA (For coating, order NBP2-34716 (BSA free version of NBP2-32901))
Reviewed Applications
Read 1 Review rated 4
using
NBP2-32901 in the following applications:

Reactivity Notes

Shows broad species reactivity.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for SUMO1 Antibody (SM1/495)

  • DAP1
  • GAP modifying protein 1
  • GAP-modifying protein 1
  • GMP1SMT3CSMT3H3OFC10UBL1PIC1
  • PIC1
  • SENP2
  • Sentrin
  • small ubiquitin-related modifier 1
  • SMT3 homolog 3
  • SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
  • SMT3 suppressor of mif two 3 homolog 1 (yeast)
  • SMT3
  • SMT3C
  • SMT3H3
  • SUMO1
  • SUMO-1
  • Ubiquitin-homology domain protein PIC1
  • ubiquitin-like 1 (sentrin)
  • Ubiquitin-like protein SMT3C
  • Ubiquitin-like protein UBL1
  • UBL1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56444
Species: Hu, Mu
Applications: WB, IHC-P
NBP2-24651
Species: Hu, Mu, Eq, Pm
Applications: WB
NBP1-86887
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56405
Species: Hu, Mu
Applications: WB, Simple Western
NB100-59787
Species: Hu, Mu
Applications: WB, IB, ICC/IF, IHC, IHC-P, IP, PLA
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
NBP1-56590
Species: Hu, Rt
Applications: WB, IHC, IF
NBP2-02623
Species: Hu, Rt, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF9024
Species: Mu, Rt
Applications: WB, IHC
NBP2-13961
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-47645
Species: Hu, Mu, Rt
Applications: Flow, IHC-P, PAGE, IF
NB100-74480
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
H00026054-M01
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P
NB600-1318
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-32901
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, PAGE

Publications for SUMO1 Antibody (NBP2-32901) (0)

There are no publications for SUMO1 Antibody (NBP2-32901).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for SUMO1 Antibody (NBP2-32901) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-32901:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SUMO1 NBP2-32901
Enlarge
reviewed by:
Anonymous
WB Human 05/17/2016
View

Summary

ApplicationWestern Blot
Sample TestedIB3-1 whole cell lysate
SpeciesHuman

Blocking

Blocking Details2% BSA in PBS with 0.1% tween20, 10 minute at RT

Primary Anitbody

Dilution Ratio1:1000 dilution in PBS with 0.1% tween20, 4 degree overnight

Secondary Antibody

Secondary Descriptionanti-moust HRP
Secondary Manufacturer Cat#Cell signaling, 7076S
Secondary Concentration1:5000 dilution

Details

Detection NotesBioRad Clarity Western ECL, 3 minute exposure

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SUMO1 Antibody (NBP2-32901) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SUMO1 Products

Bioinformatics Tool for SUMO1 Antibody (NBP2-32901)

Discover related pathways, diseases and genes to SUMO1 Antibody (NBP2-32901). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SUMO1 Antibody (NBP2-32901)

Discover more about diseases related to SUMO1 Antibody (NBP2-32901).
 

Pathways for SUMO1 Antibody (NBP2-32901)

View related products by pathway.

PTMs for SUMO1 Antibody (NBP2-32901)

Learn more about PTMs related to SUMO1 Antibody (NBP2-32901).
 

Research Areas for SUMO1 Antibody (NBP2-32901)

Find related products by research area.

Blogs on SUMO1

There are no specific blogs for SUMO1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
05/17/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol SUMO1
Entrez
Uniprot