SUMO1 Antibody (SM1/495)

Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.

Immunocytochemistry/Immunofluorescence: SUMO1 Antibody (SM1/495) [NBP2-32901] - Paraformaldehyde-fixed HepG2 cells stained with SUMO1 Antibody (SM1/495)followed by goat anti-Mouse IgG-CF488 (Green). Cell membranes are stained with Phalloidin (red).

Immunohistochemistry-Paraffin: SUMO1 Antibody (SM1/495) [NBP2-32901] - Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-1 MAb (SM1/495)

Flow Cytometry: SUMO1 Antibody (SM1/495) [NBP2-32901] - Flow Cytometric Analysis of paraformaldehyde-fixed HepG2 cells using SUMO1 Antibody (SM1/495) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: SM1/495
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SUMO1 Antibody (SM1/495) Summary

• Immunogen: Recombinant human SUMO1 protein
• Localization: Predominantly nuclear with some cytoplasmic
• Specificity: This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SUMO1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
• Application Notes: ELISA (For coating, order NBP2-34716 (BSA free version of NBP2-32901)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Reactivity Notes

Shows broad species reactivity.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for SUMO1 Antibody (SM1/495)

• DAP1
• GAP modifying protein 1
• GAP-modifying protein 1
• GMP1SMT3CSMT3H3OFC10UBL1PIC1
• PIC1
• SENP2
• Sentrin
• small ubiquitin-related modifier 1
• SMT3 homolog 3
• SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• SMT3 suppressor of mif two 3 homolog 1 (yeast)
• SMT3
• SMT3C
• SMT3H3
• SUMO1
• SUMO-1
• Ubiquitin-homology domain protein PIC1
• ubiquitin-like 1 (sentrin)
• Ubiquitin-like protein SMT3C
• Ubiquitin-like protein UBL1
• UBL1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for SUMO1 Antibody (NBP2-32901) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 05/17/2016

Summary

• Application: Western Blot
• Sample Tested: IB3-1 whole cell lysate
• Species: Human

Blocking

• Blocking Details: 2% BSA in PBS with 0.1% tween20, 10 minute at RT

Primary Anitbody

• Dilution Ratio: 1:1000 dilution in PBS with 0.1% tween20, 4 degree overnight

Secondary Antibody

• Secondary Description: anti-moust HRP
• Secondary Manufacturer Cat#: Cell signaling, 7076S
• Secondary Concentration: 1:5000 dilution

Details

• Detection Notes: BioRad Clarity Western ECL, 3 minute exposure

Bioinformatics

• Gene Symbol: SUMO1
• Uniprot:
  • P63165()