SUMO1 Antibody (SM1/495)

Images

 
Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.
Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody (SM1/495) [NBP2-32901] - Paraformaldehyde-fixed HepG2 cells stained with SUMO1 Antibody (SM1/495)followed by goat anti-Mouse IgG-CF488 (Green). Cell membranes are ...read more
Immunohistochemistry-Paraffin: SUMO1 Antibody (SM1/495) [NBP2-32901] - Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-1 MAb (SM1/495)
Flow Cytometry: SUMO1 Antibody (SM1/495) [NBP2-32901] - Flow Cytometric Analysis of paraformaldehyde-fixed HepG2 cells using SUMO1 Antibody (SM1/495) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
SM1/495
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

SUMO1 Antibody (SM1/495) Summary

Immunogen
Recombinant human SUMO1 protein (Uniprot: P63165)
Localization
Predominantly nuclear with some cytoplasmic
Specificity
This monoclonal antibody is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SUMO1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-32901 in the following applications:

Publications
Read Publications using
NBP2-32901 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Predicted to show a broad species reactivity.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34716)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for SUMO1 Antibody (SM1/495)

  • DAP1
  • GAP modifying protein 1
  • GAP-modifying protein 1
  • GMP1SMT3CSMT3H3OFC10UBL1PIC1
  • PIC1
  • SENP2
  • Sentrin
  • small ubiquitin-related modifier 1
  • SMT3 homolog 3
  • SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
  • SMT3 suppressor of mif two 3 homolog 1 (yeast)
  • SMT3
  • SMT3C
  • SMT3H3
  • SUMO1
  • SUMO-1
  • Ubiquitin-homology domain protein PIC1
  • ubiquitin-like 1 (sentrin)
  • Ubiquitin-like protein SMT3C
  • Ubiquitin-like protein UBL1
  • UBL1

Background

Sumo 1 is a ubiquitin-like protein which binds to a wide range of target proteins. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. SUMO 1 is also known to form a complex with RanGAP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SUMO1 Antibody (NBP2-32901)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
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Filter By Species
Rat
(1)
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Review for SUMO1 Antibody (NBP2-32901) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-32901:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SUMO1 NBP2-32901
Enlarge
4
reviewed by:
Seakwoo Lee
WB Human 05/17/2016
View

Summary

ApplicationWestern Blot
Sample TestedIB3-1 whole cell lysate
SpeciesHuman

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Seakwoo Lee
05/17/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol SUMO1
Uniprot