![Western Blot: SENP3 Antibody [NBP1-56590] - Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1 : 1562500 Positive control: 721_B cell lysatesENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.](http://images.novusbio.com/fullsize/SENP3-Antibody-Western-Blot-NBP1-56590-img0010.jpg "Western Blot: SENP3 Antibody [NBP1-56590] - Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1 : 1562500 Positive control: 721_B cell lysatesENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.5 mg/ml |
| Description | The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution. |
| Immunogen | Synthetic peptides corresponding to SENP3(SUMO1/sentrin/SMT3 specific peptidase 3) The peptide sequence was selected from the N terminal of SENP3.
Peptide sequence PPPKPRLKSGGGFGPDPGSGTTVPARRLPVPRPSFDASASEEEEEEEEEE. The peptide sequence for this immunogen was taken from within the described region. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SENP3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 2% Sucrose |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SENP3 |
![Immunohistochemistry: SENP3 Antibody [NBP1-56590] - HeLa cells using Immunohistochemistry. SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa.](http://images.novusbio.com/fullsize/SENP3-Antibody-Immunohistochemistry-NBP1-56590-img0008.jpg "Immunohistochemistry: SENP3 Antibody [NBP1-56590] - HeLa cells using Immunohistochemistry. SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa.")
![Western Blot: SENP3 Antibody [NBP1-56590] - Hela cell lysate, Antibody Titration: 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/SENP3-Antibody-Western-Blot-NBP1-56590-img0004.jpg "Western Blot: SENP3 Antibody [NBP1-56590] - Hela cell lysate, Antibody Titration: 0.2-1 ug/ml.")
![Western Blot: SENP3 Antibody [NBP1-56590] - Sample Type: 1. Rat Liver cells (200ug) 2. Rat Brain cells (200ug) 3. Rat Testis cells (200ug) 4. Rat Kidney cells (200ug) Primary Dilution: 1:1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1:1000 Image Submitted by: Andreia Carvalho IBMC-OBF, Portugal.](http://images.novusbio.com/fullsize/SENP3-Antibody-Western-Blot-NBP1-56590-img0005.jpg "Western Blot: SENP3 Antibody [NBP1-56590] - Sample Type: 1. Rat Liver cells (200ug) 2. Rat Brain cells (200ug) 3. Rat Testis cells (200ug) 4. Rat Kidney cells (200ug) Primary Dilution: 1:1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1:1000 Image Submitted by: Andreia Carvalho IBMC-OBF, Portugal.")
![Western Blot: SENP3 Antibody [NBP1-56590] - 1. HeLa cell extract (200ug) 2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME *HA-S2-VME-SENP3 Primary Dilution: 1:1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1:1000.](http://images.novusbio.com/fullsize/SENP3-Antibody-Western-Blot-NBP1-56590-img0006.jpg "Western Blot: SENP3 Antibody [NBP1-56590] - 1. HeLa cell extract (200ug) 2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME *HA-S2-VME-SENP3 Primary Dilution: 1:1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1:1000.")
![Western Blot: SENP3 Antibody [NBP1-56590] - Rat Liver cells (200ug) 2. Rat Brain cells (200ug) 3. Rat Testis cells (200ug) 4. Rat Kidney cells (200ug) at 1:1000.](http://images.novusbio.com/fullsize/SENP3-Antibody-Western-Blot-NBP1-56590-img0007.jpg "Western Blot: SENP3 Antibody [NBP1-56590] - Rat Liver cells (200ug) 2. Rat Brain cells (200ug) 3. Rat Testis cells (200ug) 4. Rat Kidney cells (200ug) at 1:1000.")
![Western Blot: SENP3 Antibody [NBP1-56590] - 1. HeLa cell extract (200ug) Primary Dilution: 1 : 1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1 : 1000.](http://images.novusbio.com/fullsize/SENP3-Antibody-Western-Blot-NBP1-56590-img0009.jpg "Western Blot: SENP3 Antibody [NBP1-56590] - 1. HeLa cell extract (200ug) Primary Dilution: 1 : 1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1 : 1000.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")