SENP6 Antibody (4B7)

Images

 
Western Blot: SENP6 Antibody (4B7) [H00026054-M01] - SENP6 monoclonal antibody (M01), clone 4B7 Analysis of SENP6 expression in IMR-32.
Immunohistochemistry-Paraffin: SENP6 Antibody (4B7) [H00026054-M01] - Analysis of monoclonal antibody to SENP6 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Flow Cytometry: SENP6 Antibody (4B7) [H00026054-M01] - Staining with senp6 antibody and negative controls, acquisition by FACS. Image from verified customer review.
Sandwich ELISA: SENP6 Antibody (4B7) [H00026054-M01] - Detection limit for recombinant GST tagged SENP6 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clone
4B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SENP6 Antibody (4B7) Summary

Immunogen
SENP6 (NP_056386, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag.MAAGKSGGSAGEITFLEALARSESKRDGGFKNNWSFDHEEESEGDTDKDGTNLLSVDEDEDSETSKGKKLNRRSEIVANSSGEFILKTYVRRNKSESFKTLKGNPIGLNM
Specificity
SENP6 - SUMO1/sentrin specific protease 6
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SENP6
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Flow data from customer review. ICC data from customer review.
Reviewed Applications
Read 1 Review rated 4
using
H00026054-M01 in the following applications:

Publications
Read Publications using H00026054-M01.

Reactivity Notes

Mouse data from customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SENP6 Antibody (4B7)

  • EC 3.4.22.-
  • KIAA0797FLJ11887
  • Sentrin/SUMO-specific protease SENP6
  • sentrin-specific protease 6
  • SSP1KIAA0389
  • SUMO1/sentrin specific peptidase 6,2810017C20Rik
  • SUMO1/sentrin specific protease 6
  • SUMO-1-specific protease 1
  • SUSP1FLJ11355

Background

Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1; MIM 601912), are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins (Kim et al., 2000 [PubMed 10799485]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SENP6 Antibody (H00026054-M01)(4)

Review for SENP6 Antibody (H00026054-M01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using H00026054-M01:
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Flow
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry SENP6 H00026054-M01
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reviewed by:
Anonymous
Flow Human 04/25/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman Cell lines
SpeciesHuman
Lot1182-4b7

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1:400

Details

Fixation Details2% PFA fixation
Permeabilization Details0.3% saponin permeabilization
Wash DescriptionFACS buffer with 0.1% saponin

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SENP6 Antibody (H00026054-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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4
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2
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1
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Anonymous
04/25/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol SENP6
Entrez
OMIM
Uniprot