Seladin 1 Antibody (HL3253)

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Seladin 1 antibody [HL3253] detects Seladin 1 protein by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: Seladin 1 stained by Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: HL3253
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Seladin 1 Antibody (HL3253) Summary

• Description: Novus Biologicals Rabbit Seladin 1 Antibody (HL3253) (NBP3-48781) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the Intracellular domain of human Seladin 1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DHCR24
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Seladin 1 Antibody (HL3253)

• 24-dehydrocholesterol reductaseNbla03646
• DCE
• desmosterol-to-cholesterol enzyme
• Diminuto/dwarf1 homolog
• EC 1.3.1.72
• KIAA0018SELADIN1
• seladin 1,3-beta-hydroxysterol delta-24-reductase
• seladin-1
• selective AD indicator 1,3 beta-hydroxysterol delta 24-reductase

Background

Seladin 1 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHCR24