CYP46A1 Antibody

Images

 
Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140.
Immunocytochemistry/ Immunofluorescence: CYP46A1 Antibody [NB400-140] - CYP46A1 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Western Blot: CYP46A1 Antibody [NB400-140] - Detection of Cyp46 in human brain lysates.
Western Blot: CYP46A1 Antibody [NB400-140] - Analysis of Cyp-46 in HEK293 cells overexpressing CYP46A1 or transfected with an empty vector using NB400-140.

Product Details

Summary
Reactivity Hu, Rb, Mu(-)Species Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CYP46A1 Antibody Summary

Immunogen
A synthetic peptide corresponding to residues between 200-250 of human CYP46A1. [UniProt# Q9Y6A2]
Localization
Endoplasmic reticulum
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP46A1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This CYP46A1 antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot and Immunohistochemistry paraffin embedded sections. In ICC/IF endoplasmic reticulum staining was observed.
Positive Control
CYP46A1 Lysate (NBL1-09694)
Publications
Read Publication using
NB400-140 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Human and Rabbit. Does not react with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for CYP46A1 Antibody

  • CH24H
  • cholesterol 24-hydroxylase
  • CYP46CP46
  • Cytochrome P450 46A1
  • cytochrome P450, family 46, subfamily A, polypeptide 1
  • cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
  • EC 1.14.13.98

Background

Cholesterol homeostasis in brain relies on a unique catabolic pathway which involves neuronal specific CYP46A1 (cytochrome P450 46A1) mediated conversion of cholesterol into 24(S)-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol, the most important mechanism of cholesterol turnover. While cholesterol cannot pass blood brain barrier (BBB), the major cholesterol elimination product from brain tissues is 24S-hydroxycholesterol which has the ability to pass BBB into circulation via ABCA1 (the origin of more than 90 % of plasma 24S-hydroxycholesterol). CYP46A1 belongs to cytochrome P450 family of enzymes and it localize to ER/microsomes as single-pass membrane protein, and unlike other cytochrome P450 sterol hydroxylases that are predominantly expressed in the liver, CYP46A1 is highly expressed in neurons of several brain regions as well as of neural retina. CYP46A1 is involved in memory and cognition through its effect on cerebral cholesterol turnover and a single nucleotide polymorphism T/C (rs754203) in intron 2 of CYP46A1 has been discovered as responsible for increased susceptibility to Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-60531
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NBP2-13075
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
NB400-105
Species: Hu, Mu, Rt, Po, Ca, Ch, Ha, Md
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, GS
NBP2-32433
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-25162
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-42388
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-37502
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
MAB9120
Species: Hu
Applications: ICC
NBP1-32247
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-09138
Species: Hu
NB400-157
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western
NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NB100-74398
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB4077
Species: Hu
Applications: WB, IHC
NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-50216
Species: Hu
Applications: WB, ELISA, IHC
NB100-1596
Species: Hu, Pm, Rb
Applications: WB, ICC/IF, IHC
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, RIA

Publications for CYP46A1 Antibody (NB400-140)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for CYP46A1 Antibody (NB400-140) (0)

There are no reviews for CYP46A1 Antibody (NB400-140). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYP46A1 Antibody (NB400-140) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional CYP46A1 Products

Bioinformatics Tool for CYP46A1 Antibody (NB400-140)

Discover related pathways, diseases and genes to CYP46A1 Antibody (NB400-140). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CYP46A1 Antibody (NB400-140)

Discover more about diseases related to CYP46A1 Antibody (NB400-140).
 

Pathways for CYP46A1 Antibody (NB400-140)

View related products by pathway.

PTMs for CYP46A1 Antibody (NB400-140)

Learn more about PTMs related to CYP46A1 Antibody (NB400-140).
 

Research Areas for CYP46A1 Antibody (NB400-140)

Find related products by research area.

Blogs on CYP46A1

There are no specific blogs for CYP46A1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CYP46A1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CYP46A1
Entrez
Uniprot