Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140 in HEK293 cells overexpressing CYP46A1.
Immunocytochemistry/ Immunofluorescence: CYP46A1 Antibody [NB400-140] - CYP46A1 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Western Blot: CYP46A1 Antibody [NB400-140] - Detection of Cyp46 in human brain lysates.
Western Blot: CYP46A1 Antibody [NB400-140] - Analysis of Cyp-46 in HEK293 cells overexpressing CYP46A1 or transfected with an empty vector using NB400-140.
Cholesterol homeostasis in brain relies on a unique catabolic pathway which involves neuronal specific CYP46A1 (cytochrome P450 46A1) mediated conversion of cholesterol into 24(S)-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol, the most important mechanism of cholesterol turnover. While cholesterol cannot pass blood brain barrier (BBB), the major cholesterol elimination product from brain tissues is 24S-hydroxycholesterol which has the ability to pass BBB into circulation via ABCA1 (the origin of more than 90 % of plasma 24S-hydroxycholesterol). CYP46A1 belongs to cytochrome P450 family of enzymes and it localize to ER/microsomes as single-pass membrane protein, and unlike other cytochrome P450 sterol hydroxylases that are predominantly expressed in the liver, CYP46A1 is highly expressed in neurons of several brain regions as well as of neural retina. CYP46A1 is involved in memory and cognition through its effect on cerebral cholesterol turnover and a single nucleotide polymorphism T/C (rs754203) in intron 2 of CYP46A1 has been discovered as responsible for increased susceptibility to Alzheimer's disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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