BACE-1 Antibody (137612)

Images

 
Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse BACE‑1 Ectodomain Monoclonal Antibody (Catalog # MAB931) followed by ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, CyTOF-ready, Flow
Clone
137612
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Suitable Sample Type
23038

Order Details

BACE-1 Antibody (137612) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human BACE-1
Thr22-Tyr460
Accession # P56817
Specificity
Detects human and mouse BACE-1 in direct ELISAs and human BACE-1 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BACE-2, recombinant mouse (rm) BACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BACE1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB931 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BACE-1 Antibody (137612)

  • APP beta-secretase
  • ASP2
  • Aspartyl protease 2
  • BACE1
  • BACE-1
  • BACEAsp 2
  • beta-secretase 1 precursor variant 1
  • beta-secretase 1
  • beta-site amyloid beta A4 precursor protein-cleaving enzyme
  • Beta-site amyloid precursor protein cleaving enzyme 1
  • Beta-site APP cleaving enzyme 1
  • beta-site APP-cleaving enzyme 1
  • beta-site APP-cleaving enzyme
  • EC 3.4.23
  • EC 3.4.23.46
  • FLJ90568
  • HSPC104
  • KIAA1149
  • memapsin-2
  • Membrane-associated aspartic protease 2
  • transmembrane aspartic proteinase Asp2

Background

BACE-1 (beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is the major beta secretase, and together with the gamma  secretase, is responsible for generating the amyloid beta peptide (A beta ) from the amyloid precursor protein (APP) (3, 4). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta -dependent but also A beta -independent pathogenic mechanisms (5). In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins (6‑8).
  1. Vassar, R. et al. (1999) Science 286:735.
  2. Yan, R. et al. (1999) Nature 402:533.
  3. Cai, H. et al. (2001) Nature Neurosci. 4:233.
  4. Roberds, S.L. et al. (2001) Human Mol. Genet. 97:1317.
  5. Rockenstein, E. et al. (2005) J. Biol. Chem. 280:32957.
  6. Li, Q and T.C. Sudhof (2004) J. Biol. Chem. 279:10542.
  7. Lichtenthaler, S.F. et al. (2003) J. Biol. Chem. 278:48713.
  8. Kitazynem, S. et al. (2005) J. Biol. Chem. 280:8589.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BACE-1 Antibody (MAB931)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ELISA Development, Western Blot.


Filter By Application
ELISA Development
(2)
Western Blot
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB931 Applications Species
MI Khan, JH Shin, MY Kim, TS Shin, JD Kim Green Tea Seed Isolated Theasaponin E1 Ameliorates AD Promoting Neurotoxic Pathogenesis by Attenuating Abeta Peptide Levels in SweAPP N2a Cells Molecules, 2020;25(10):. 2020 [PMID: 32429462] (Western Blot, Mouse) Western Blot Mouse
J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv, 2020;6(9):eaax3223. 2020 [PMID: 32133394] (Western Blot, Human) Western Blot Human
A Mendsaikha, I Tooyama, JP Bellier, GE Serrano, LI Sue, LF Lue, TG Beach, DG Walker Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer's disease and aged brains: increased levels correlate with neuropathology Acta Neuropathol Commun, 2019;7(1):215. 2019 [PMID: 31864418] (Western Blot, Human) Western Blot Human
Schnoder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y Deficiency of Neuronal p38alpha MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1. J Biol Chem, 2015;291(5):2067-79. 2015 [PMID: 26663083] (Western Blot, Human) Western Blot Human
Wang Y, Thinakaran G, Kar S Overexpression of the IGF-II/M6P receptor in mouse fibroblast cell lines differentially alters expression profiles of genes involved in Alzheimer's disease-related pathology. PLoS ONE, 2014;9(5):e98057. 2014 [PMID: 24846272] (Western Blot, Mouse) Western Blot Mouse
Cheng X, He P, Lee T, Yao H, Li R, Shen Y High activities of BACE1 in brains with mild cognitive impairment. Am J Pathol, 2014;184(1):141-7. 2014 [PMID: 24332014] (ELISA Development, Human) ELISA Development Human
Ahmed RR, Holler CJ, Webb RL BACE1 and BACE2 enzymatic activities in Alzheimer's disease. J. Neurochem., 2010;112(4):1045-53. 2010 [PMID: 19968762] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol BACE1
Uniprot