SAT1 Antibody

Western Blot: SAT1 Antibody [NB110-41622] - Detection of SAT1 in human SAT1 transfected lysate.

Immunohistochemistry: SAT1 Antibody [NB110-41622] - IHC analysis of SAT1 in mouse seminal vesical (left) and prostate (right) usind DAB with hematoxylin counterstain,

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Gt, Xp
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SAT1 Antibody Summary

• Immunogen: A synthetic peptide made to a C-terminal region within residues 100-171 of human SAT1. [Swiss-Prot# P21673]
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
• Application Notes: This SAT1 antibody is useful for Western Blot, and Immunohistochemistry paraffin embedded sections. In Western Blot analysis on transfected lysates a band is seen at ~27 kDa. In IHC-P, strong staining was observed in the cytoplasm with some weak nuclear staining in mouse seminal vesical and prostate tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SAT1 Overexpression Lysate

Reactivity Notes

Immunogen sequence has 86% homology to Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SAT1 Antibody

• DC21
• diamine acetyltransferase 1
• diamine N-acetyltransferase 1
• EC 2.3.1.57
• KFSD
• Polyamine N-acetyltransferase 1
• Putrescine acetyltransferase
• SAT
• Spermidine/spermine N(1)-acetyltransferase 1
• spermidine/spermine N1-acetyltransferase 1
• spermidine/spermine N1-acetyltransferase alpha
• spermidine/spermine N1-acetyltransferase
• SSAT-1
• SSATKFSDX

Background

SAT1 or spermidine/spermine N(1)-acetyltransferase 1 (also called SSAT, diamine acetyltransferase 1 and putrescine acetyltransferase) is a homodimeric cytoplasmic enzyme which belongs to acetyltransferase family and catalyzes acetylation of polyamines. SAT1's substrate specificity order is: norspermidine = spermidine >spermine > N(1)-acetylspermine > putrescine, and its activity is highly regulated that allows a fine attenuation of the intracellular polyamines concentrations via acetylation or polyamine export. Polyamines such as putrescine, spermidine and spermine are implicated in the regulation of cellular growth/proliferation (increased levels leads to cancer progression) and their levels are controlled by several catabolic enzymes which includes such as SAT1, APAO (acetylpolyamine oxidase) and SMO (spermine oxidase). Enhanced SSAT expression has been shown to be associated with apoptosis in cancer cells and SSAT was shown to decrease the expression of anti-apoptosis-related signaling proteins AKT and mTOR. SAT1 has also been implicated in the neurobiology of suicide and decreased SAT1 expression has been observed in several brain regions in suicide completers. Defects in SAT1 may be a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAT1 Antibody (NB110-41622)(3)

Publications using NB110-41622

• Tate PM, Mastrodomenico V, Mounce BC Ribavirin Induces Polyamine Depletion via Nucleotide Depletion to Limit Virus Replication Cell Rep Sep 3 2019 [PMID: 31484073]
• Lane DJR, Bae DH, Siafakas AR et al. Coupling of the polyamine and iron metabolism pathways in the regulation of proliferation: Mechanistic links to alterations in key polyamine biosynthetic and catabolic enzymes Biochim. Biophys. Acta May 16 2018 [PMID: 29777905] (WB)
• Liao C-P, Lasbury ME, Wang S-H et al. Pneumocystis Mediates Overexpression of Antizyme Inhibitor Resulting in Increased Polyamine Levels Apoptosis in Alveolar Macrophages. J Biol Chem;284(12):8174-8184. 2009 [PMID: 19158080] (WB, Rat)

FAQs for SAT1 Antibody (NB110-41622). (Showing 1 - 1 of 1 FAQs).

1. I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?
   • I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in <a href="http://www.jbc.org/content/284/12/8174.long" target="_blank">The Journal of Biological Chemistry</a> 2009;284(12):8174-8184.

Bioinformatics

• Gene Symbol: SAT1