SAT1 Antibody

Images

 
Western Blot: SAT1 Antibody [NB110-41622] - Detection of SAT1 in human SAT1 transfected lysate.
Immunohistochemistry: SAT1 Antibody [NB110-41622] - IHC analysis of SAT1 in mouse seminal vesical (left) and prostate (right) usind DAB with hematoxylin counterstain,

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Gt, XpSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SAT1 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region within residues 100-171 of human SAT1. [Swiss-Prot# P21673]
Localization
Cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This SAT1 antibody is useful for Western Blot, and Immunohistochemistry paraffin embedded sections. In Western Blot analysis on transfected lysates a band is seen at ~27 kDa. In IHC-P, strong staining was observed in the cytoplasm with some weak nuclear staining in mouse seminal vesical and prostate tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
SAT1 Overexpression Lysate
Control Peptide
Publications
Read Publications using
NB110-41622 in the following applications:

  • WB
    2 publications

Reactivity Notes

Immunogen sequence has 86% homology to Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAT1 Antibody

  • DC21
  • diamine acetyltransferase 1
  • diamine N-acetyltransferase 1
  • EC 2.3.1.57
  • KFSD
  • Polyamine N-acetyltransferase 1
  • Putrescine acetyltransferase
  • SAT
  • Spermidine/spermine N(1)-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase alpha
  • spermidine/spermine N1-acetyltransferase
  • SSAT-1
  • SSATKFSDX

Background

SAT1 or spermidine/spermine N(1)-acetyltransferase 1 (also called SSAT, diamine acetyltransferase 1 and putrescine acetyltransferase) is a homodimeric cytoplasmic enzyme which belongs to acetyltransferase family and catalyzes acetylation of polyamines. SAT1's substrate specificity order is: norspermidine = spermidine >spermine > N(1)-acetylspermine > putrescine, and its activity is highly regulated that allows a fine attenuation of the intracellular polyamines concentrations via acetylation or polyamine export. Polyamines such as putrescine, spermidine and spermine are implicated in the regulation of cellular growth/proliferation (increased levels leads to cancer progression) and their levels are controlled by several catabolic enzymes which includes such as SAT1, APAO (acetylpolyamine oxidase) and SMO (spermine oxidase). Enhanced SSAT expression has been shown to be associated with apoptosis in cancer cells and SSAT was shown to decrease the expression of anti-apoptosis-related signaling proteins AKT and mTOR. SAT1 has also been implicated in the neurobiology of suicide and decreased SAT1 expression has been observed in several brain regions in suicide completers. Defects in SAT1 may be a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAT1 Antibody (NB110-41622)(2)

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Product General Protocols

Video Protocols

WB Video Protocol

FAQs for SAT1 Antibody (NB110-41622). (Showing 1 - 1 of 1 FAQs).

  1. I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?
    • I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in <a href="http://www.jbc.org/content/284/12/8174.long" target="_blank">The Journal of Biological Chemistry</a> 2009;284(12):8174-8184.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-41622AF405
Alexa Fluor 488 Labeled NB110-41622AF488
Alexa Fluor 647 Labeled NB110-41622AF647
Alexa Fluor 700 Labeled NB110-41622AF700
Biotin Labeled NB110-41622B
DyLight 405 Labeled NB110-41622V
DyLight 488 Labeled NB110-41622G
DyLight 550 Labeled NB110-41622R
DyLight 650 Labeled NB110-41622C
FITC Labeled NB110-41622F
HRP Labeled NB110-41622H

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Blogs on SAT1

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Contact Information

Product PDFs

Calculators

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Bioinformatics

Gene Symbol SAT1