SAT1 Antibody [FITC]


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Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Ch, Gt, XpSpecies Glossary
Applications WB, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

SAT1 Antibody [FITC] Summary

A synthetic peptide made to a C-terminal region within residues 100-171 of human SAT1. [Swiss-Prot# P21673]
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
FITC (A=495, E=519)
0.05% Sodium Azide
Immunogen affinity purified


  • Western Blot 2 ug/ml
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This SAT1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot analysis on transfected lysates a band is seen at ~27 kDa. ICC/IF, cytoplasmic staining was observed in U2OS cells. In IHC-P, strong staining was observed in the cytoplasm with some weak nuclear staining in mouse seminal vesical and prostate tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen sequence has 86% homology to Zebrafish.

Alternate Names for SAT1 Antibody [FITC]

  • DC21
  • diamine acetyltransferase 1
  • diamine N-acetyltransferase 1
  • EC
  • KFSD
  • Polyamine N-acetyltransferase 1
  • Putrescine acetyltransferase
  • SAT
  • Spermidine/spermine N(1)-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase alpha
  • spermidine/spermine N1-acetyltransferase
  • SSAT-1


SAT1 or spermidine/spermine N(1)-acetyltransferase 1 (also called SSAT, diamine acetyltransferase 1 and putrescine acetyltransferase) is a homodimeric cytoplasmic enzyme which belongs to acetyltransferase family and catalyzes acetylation of polyamines. SAT1's substrate specificity order is: norspermidine = spermidine >spermine > N(1)-acetylspermine > putrescine, and its activity is highly regulated that allows a fine attenuation of the intracellular polyamines concentrations via acetylation or polyamine export. Polyamines such as putrescine, spermidine and spermine are implicated in the regulation of cellular growth/proliferation (increased levels leads to cancer progression) and their levels are controlled by several catabolic enzymes which includes such as SAT1, APAO (acetylpolyamine oxidase) and SMO (spermine oxidase). Enhanced SSAT expression has been shown to be associated with apoptosis in cancer cells and SSAT was shown to decrease the expression of anti-apoptosis-related signaling proteins AKT and mTOR. SAT1 has also been implicated in the neurobiology of suicide and decreased SAT1 expression has been observed in several brain regions in suicide completers. Defects in SAT1 may be a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SAT1 Antibody (NB110-41622F). (Showing 1 - 1 of 1 FAQ).

  1. I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?
    • I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in <a href="" target="_blank">The Journal of Biological Chemistry</a> 2009;284(12):8174-8184.

Secondary Antibodies


Isotype Controls

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Blogs on SAT1

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Contact Information

Product PDFs


Gene Symbol SAT1

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