SAT1 Antibody [DyLight 488]

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Gt, Xp
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488

SAT1 Antibody [DyLight 488] Summary

• Immunogen: A synthetic peptide made to a C-terminal region within residues 100-171 of human SAT1. [Swiss-Prot# P21673]
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This SAT1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot analysis on transfected lysates a band is seen at ~27 kDa. ICC/IF, cytoplasmic staining was observed in U2OS cells. In IHC-P, strong staining was observed in the cytoplasm with some weak nuclear staining in mouse seminal vesical and prostate tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SAT1 Antibody [DyLight 488]

• DC21
• diamine acetyltransferase 1
• diamine N-acetyltransferase 1
• EC 2.3.1.57
• KFSD
• Polyamine N-acetyltransferase 1
• Putrescine acetyltransferase
• SAT
• Spermidine/spermine N(1)-acetyltransferase 1
• spermidine/spermine N1-acetyltransferase 1
• spermidine/spermine N1-acetyltransferase alpha
• spermidine/spermine N1-acetyltransferase
• SSAT-1
• SSATKFSDX

Background

Spermidine/spermine-N1-acetyltransferase (SSAT1) is a short-lived polyamine catabolic enzyme which catalyzes the acetylation of polyamines. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Induction of SSAT1 plays an important role in polyamine homoeostasis, since the N1-acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SAT1 Antibody (NB110-41622G). (Showing 1 - 1 of 1 FAQ).

1. I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?
   • I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in <a href="http://www.jbc.org/content/284/12/8174.long" target="_blank">The Journal of Biological Chemistry</a> 2009;284(12):8174-8184.

Bioinformatics

• Gene Symbol: SAT1