SAT1 Antibody [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Gt, XpSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488

SAT1 Antibody [DyLight 488] Summary

Immunogen
A synthetic peptide made to a C-terminal region within residues 100-171 of human SAT1. [Swiss-Prot# P21673]
Localization
Cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SAT1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This SAT1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot analysis on transfected lysates a band is seen at ~27 kDa. ICC/IF, cytoplasmic staining was observed in U2OS cells. In IHC-P, strong staining was observed in the cytoplasm with some weak nuclear staining in mouse seminal vesical and prostate tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen sequence has 86% homology to Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SAT1 Antibody [DyLight 488]

  • DC21
  • diamine acetyltransferase 1
  • diamine N-acetyltransferase 1
  • EC 2.3.1.57
  • KFSD
  • Polyamine N-acetyltransferase 1
  • Putrescine acetyltransferase
  • SAT
  • Spermidine/spermine N(1)-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase 1
  • spermidine/spermine N1-acetyltransferase alpha
  • spermidine/spermine N1-acetyltransferase
  • SSAT-1
  • SSATKFSDX

Background

Spermidine/spermine-N1-acetyltransferase (SSAT1) is a short-lived polyamine catabolic enzyme which catalyzes the acetylation of polyamines. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Induction of SSAT1 plays an important role in polyamine homoeostasis, since the N1-acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-41622G
Species: Hu, Mu, Rt, Po, Bv, Ch, Gt, Xp
Applications: WB, IHC, IHC-P

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SAT1 Antibody (NB110-41622G). (Showing 1 - 1 of 1 FAQ).

  1. I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?
    • I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in <a href="http://www.jbc.org/content/284/12/8174.long" target="_blank">The Journal of Biological Chemistry</a> 2009;284(12):8174-8184.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB110-41622B
DyLight 550 Labeled NB110-41622R
DyLight 650 Labeled NB110-41622C
HRP Labeled NB110-41622H

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Bioinformatics Tool for SAT1 Antibody (NB110-41622G)

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Research Areas for SAT1 Antibody (NB110-41622G)

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Blogs on SAT1

There are no specific blogs for SAT1, but you can read our latest blog posts.

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Bioinformatics

Gene Symbol SAT1