SALL4 Antibody (CL12183) - Azide and BSA Free

Staining of human cerebellum shows no positivity in Purkinje cells as expected.

Analysis in human testis and endometrium tissues using NBP3-44348 antibody. Corresponding SALL4 RNA-seq data are presented for the same tissues.

Staining of human endometrium shows no positivity in glandular cells as expected.

Staining of human ovary shows strong nuclear positivity in oocyte.

Staining of mouse embryo E11 shows moderate to strong nuclear positivity in in a subset of cells in developing hindbrain.

Staining of human testis cancer (embryonal carcinoma) shows strong nuclear positivity in tumor cells.

Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.

Analysis in human cell line NTERA-2.

Staining of mouse embryo E11 shows strong nuclear positivity in cells in developing testis.

Staining of NTERA-2 cells using the Anti-SALL4 monoclonal antibody) .

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: CL12183
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SALL4 Antibody (CL12183) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of Q9UJQ4, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SALL4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for SALL4 Antibody (CL12183) - Azide and BSA Free

• dJ1112F19.1
• DRRS
• HSAL4
• MGC133050
• SALL4
• sal-like 4 (Drosophila)
• sal-like protein 4
• Zinc finger protein 797
• Zinc finger protein SALL4
• ZNF797
• ZNF797sal (Drosophila)-like 4

Background

Sall4 is encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SALL4