S100A1 Antibody (4C4.9)


Western Blot: S100A1 Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: S100A1 Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100 Ab (4C4.9).
Immunohistochemistry-Paraffin: S100A1 Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100 4C4.9 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Note ...read more

Product Details

Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
0.2 mg/ml

S100A1 Antibody (4C4.9) Summary

S100 protein purified from bovine brain was used as the immunogen for the S100 4C4.9 antibody.
Astrocyte Marker
S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein. Despite the fact that S-100 protein is an ubiquitous substance, its demonstration is of great value in the identification of several neoplasms, particularly melanomas and their metastases.
IgG2a Kappa
Protein A purified
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  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
Application Notes
Human Chromosome Location: 1q21.3
Suitable for formalin fixed tissues. No treatment is required. This antibody stains S100 protein. This antibody stains Schwannomas, ependymomas, astrogliomas; almost all benign and malignant melanomas and their metastases.
Immunohistochemistry-Frozen and Immunocytochemistry/Immunofluorescence applications cited in literature. Use in Immunohistochemistry reported in scientific literature (PMID 25357029). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP2-29403 in the following applications:

Packaging, Storage & Formulations

Store at 4C.
10 mM PBS with 0.05% BSA
0.05% Sodium Azide
0.2 mg/ml
Protein A purified

Alternate Names for S100A1 Antibody (4C4.9)

  • protein S100-A1
  • S100 alpha
  • S100 calcium binding protein A1
  • S100 calcium-binding protein A1S100
  • S-100 protein alpha chain
  • S-100 protein subunit alpha
  • S100 protein, alpha polypeptide
  • S100A
  • S100A1
  • S100-alpha


The protein encoded by this gene is a member of the S100 family of proteins which is a multigenic family of non-ubiquitous Ca(+)-modulated proteins of the EF-hand type expressed in vertebrates exclusively with molecular masses ranging from 10 to 12 kDa. Within cells, most of S100 members exist in the form of antiparallelly packed homodimers, capable of functionally crossbridging two homologous or heterologous target proteins in a Ca(+)-dependent manner. It constitutes a subgroup of Ca(+)-binding proteins of the EF-hand type comprising three dimeric isoforms, S100a0, S100a and S100b. This protein function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Overexpression of S100B in the brain of individuals with Down's syndrome and in the brain of patients with Alzheimer's disease and AIDS has led to the hypothesis that S100B plays a role in common neuro-pathologies associated with these diseases and other diseases like Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. It is considered an astrocytic in-situ marker and protein levels in cerebrospinal fluid or serum are often used as biomarker for astrocytic damage or dysfunction.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A1 Antibody (NBP2-29403)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33168AF350
Alexa Fluor 488 NBP2-33168AF488
Alexa Fluor 532 NBP2-33168AF532
Alexa Fluor 594 NBP2-33168AF594
Alexa Fluor 700 NBP2-33168AF700
Alexa Fluor 750 NBP2-33168AF750
DyLight 594 NBP2-33168DL594
DyLight 755 NBP2-33168IR
FITC NBP2-33168F
Janelia Fluor 549 NBP2-33168JF549
Janelia Fluor 646 NBP2-33168JF646

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Gene Symbol S100A1