RPSA Antibody

Western Blot: RPSA Antibody [NBP1-33002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - CA922 xenograft, using RPSA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: RPSA Antibody [NBP1-33002] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.

Western Blot: RPSA Antibody [NBP1-33002] - Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody diluted by 1:1000

Western Blot: RPSA Antibody [NBP1-33002] - Liver protein expression in different experimental groups of Laminin R, AdipoR2 and SIRT1.Western Blotting analysis of protein expression in the liver on different experimental groups of aminin Receptor. Image shows demonstrative bands of the analyzed proteins and respective housekeeping protein (beta-tubulin) in liver. Data are expressed in mean +/- s.e.m. *p<0.05 Control diet and EGCG (CE) group versus Control diet and Water (CW) group. #p<0.05 High-fat diet and EGCG (HFE) group versus HFW group. $p<0.05 HFW versus CW. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141227), licensed under a CC-BY licence.

Western Blot: RPSA Antibody [NBP1-33002] - Sample (30 ug of whole cell lysate). A: Jurkat. 12% SDS PAGE. RPSA Antibody diluted at 1:1000

Western Blot: RPSA Antibody [NBP1-33002] - RPSA antibody detects RPSA protein by Western blot analysis. Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with RPSA antibody diluted by 1:1000.

Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - Mouse small intestine. RPSA antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, RM, Sh, Xp, Ze
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RPSA Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RPSA. The exact sequence is proprietary.
• Localization: Cell membrane, Cytoplasm, Nucleus
• Predicted Species: Rat (99%), Porcine (100%), Bovine (99%), Rhesus Macaque (100%), Zebrafish (92%), Chicken (98%), Canine (100%), Sheep (99%), Xenopus (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPSA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol (pH7).
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for RPSA Antibody

• 37 kDa laminin receptor precursor
• 37/67 kDa laminin receptor
• 37LRPNEM/1CHD4
• 67 kDa laminin receptor
• 67kD, ribosomal protein SA
• 67LR
• Colon carcinoma laminin-binding protein
• LAMBR37 kDa laminin receptor
• laminin binding protein
• laminin receptor 1 (67kD, ribosomal protein SA)
• Laminin receptor 1
• Laminin-binding protein precursor p40
• LAMR 1
• lamR
• LAMR140S ribosomal protein SA
• LBP
• LBP/p40
• LRP
• LRP/LR
• Multidrug resistance-associated protein MGr1-Ag
• p40
• ribosomal protein SA

Background

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RPSA Antibody (NBP1-33002)(2)

Publications using NBP1-33002

• Santamarina AB, Oliveira JL, Silva FP et al. Green Tea Extract Rich in Epigallocatechin-3-Gallate Prevents Fatty Liver by AMPK Activation via LKB1 in Mice Fed a High-Fat Diet. PLoS ONE. 2015-11-05 [PMID: 26536464] (WB, Mouse)
• Song T, Choi CH, Cho YJ et al. Expression of 67-kDa laminin receptor was associated with tumor progression and poor prognosis in epithelial ovarian cancer. Gynecologic Oncology. 2012-01-28 [PMID: 22285843]

Bioinformatics

• Gene Symbol: RPSA
• Entrez:
  • 3921(Human)
• Uniprot:
  • P08865(Human)