Recombinant Mouse IL-12 Protein


Recombinant Mouse IL-12 (Catalog # 419‑ML) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01‑0.1 ng/mL.
1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 25 kDa and 43 kDa (R) and 68 kDa (NR) .

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Recombinant Mouse IL-12 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.01-0.1 ng/mL.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-12 protein
Mouse IL-12 p40
Accession # P43432
Mouse IL-12 p35
Accession # NP_032377
N-terminus C-terminus
Accession #
N-terminal Sequence

Met23 (p40) & Arg23 (p35)

Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
70 kDa, non-reducing conditions
Read Publications using
419-ML in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35


Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

Publications for IL-12 (419-ML)(263)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo.

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Showing Publications 1 - 10 of 263. Show All 263 Publications.
Publications using 419-ML Applications Species
Petersen-Cherubini, CL;Murphy, SP;Xin, M;Liu, Y;Deffenbaugh, JL;Jahan, I;Rau, CN;Yang, Y;Lovett-Racke, AE; Autotaxin in encephalitogenic CD4 T cells as a therapeutic target for multiple sclerosis European journal of immunology 2023-10-18 [PMID: 37850588] (Bioassay, Mouse) Bioassay Mouse
Rangel Rivera, GO;Dwyer, CJ;Knochelmann, HM;Smith, AS;Aksoy, BA;Cole, AC;Wyatt, MM;Kumaresan, S;Thaxton, JE;Lesinski, GB;Paulos, CM; Progressively enhancing stemness of adoptively transferred T cells with PI3K? blockade improves metabolism and anti-tumor immunity Cancer research 2023-10-06 [PMID: 37801615] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
Santosa, EK;Kim, H;Rückert, T;Le Luduec, JB;Abbasi, AJ;Wingert, CK;Peters, L;Frost, JN;Hsu, KC;Romagnani, C;Sun, JC; Control of nutrient uptake by IRF4 orchestrates innate immune memory Nature immunology 2023-09-11 [PMID: 37697097] (Bioassay, Mouse) Bioassay Mouse
van Os, BW;Vos, WG;Bosmans, LA;van Tiel, CM;Toom, MD;Beckers, L;Admiraal, M;Hoeksema, MA;de Winther, MP;Lutgens, E; CD40L modulates CD4+ T cell activation through receptor for activated C kinase 1 European journal of immunology 2023-09-08 [PMID: 37683186] (Bioassay, Mouse) Bioassay Mouse
Fazazi, MR;Ruda, GF;Brennan, PE;Rangachari, M; The X-linked histone demethylases KDM5C and KDM6A as regulators of T cell-driven autoimmunity in the central nervous system Brain research bulletin 2023-08-30 [PMID: 37657612] (Bioassay, Mouse) Bioassay Mouse
Peng, X;Zhang, Y;Bai, X;Li, X;Zhao, R; Phasic regulation of the ATP/P2X7 receptor signaling pathway affects the function of antigen-presenting cells in experimental autoimmune uveitis International immunopharmacology 2023-05-02 [PMID: 37141671] (Bioassay, Mouse) Bioassay Mouse
KA Read, DM Jones, S Pokhrel, EDS Hales, A Varkey, JA Tuazon, CD Eisele, O Abdouni, A Saadey, MR Leonard, RT Warren, MD Powell, JM Boss, EA Hemann, JS Yount, G Xin, HE Ghoneim, CJ Lio, AG Freud, PL Collins, KJ Oestreich Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity Nature Communications, 2023-03-24;14(1):1652. 2023-03-24 [PMID: 36964178] (Bioassay, Mouse) Bioassay Mouse
Y Liao, L Sun, M Nie, J Li, X Huang, S Heng, W Zhang, T Xia, Z Guo, Q Zhao, LJ Zhang Modulation of Skin Inflammatory Responses by Aluminum Adjuvant Pharmaceutics, 2023-02-08;15(2):. 2023-02-08 [PMID: 36839900] (Bioassay, Mouse) Bioassay Mouse
WL Yang, W Qiu, T Zhang, K Xu, ZJ Gu, Y Zhou, HJ Xu, ZZ Yang, B Shen, YL Zhao, Q Zhou, Y Yang, W Li, PY Yang, YG Yang Nsun2 coupling with RoRgammat shapes the fate of Th17 cells and promotes colitis Nature Communications, 2023-02-16;14(1):863. 2023-02-16 [PMID: 36792629] (Bioassay, Mouse) Bioassay Mouse
X Hu, Y Zou, DA Copland, LP Schewitz-B, Y Li, PJP Lait, M Stimpson, Z Zhang, S Guo, J Liang, T Chen, JJ Li, S Yuan, S Li, P Zhou, Y Liu, AD Dick, X Wen, RWJ Lee, L Wei Epigenetic drug screen identified IOX1 as an inhibitor of Th17-mediated inflammation through targeting TET2 EBioMedicine, 2022-11-03;86(0):104333. 2022-11-03 [PMID: 36335665] (Bioassay, Mouse, Transgenic Mouse) Bioassay Mouse, Transgenic Mouse
Show All 263 Publications.

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