Recombinant Mouse IL-12 Protein

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1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 25 kDa and 43 kDa (R) and 68 kDa (NR).
Recombinant Mouse IL-12 (Catalog # 419‑ML) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01‑0.1 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications BA

Order Details

Recombinant Mouse IL-12 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.01-0.1 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived
Mouse IL-12 p40
(Met23-Ser335)
Accession # P43432
Mouse IL-12 p35
(Arg23-Ala215)
Accession # NP_032377
N-terminus C-terminus
Accession #
N-terminal Sequence

Met23 (p40) & Arg23 (p35)

Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il12a
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

SDS-PAGE
70 kDa, non-reducing conditions
Publications
Read Publications using
419-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

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Publications for (157)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 5 applications: Bioassay, ELISA Standard, In Vivo, cell culture, celll culture.


Filter By Application
Bioassay
(125)
ELISA Standard
(7)
In Vivo
(20)
cell culture
(1)
celll culture
(1)
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Filter By Species
Human
(1)
Mouse
(148)
Rat
(1)
N/A
(6)
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Showing Publications 1 - 10 of 157. Show All 157 Publications.
Publications using 419-ML Applications Species
KA Read, MD Powell, CE Baker, BK Sreekumar, VM Ringel-Sca, H Bachus, RE Martin, ID Cooley, IC Allen, A Ballestero, KJ Oestreich Integrated STAT3 and Ikaros Zinc Finger Transcription Factor Activities Regulate Bcl-6 Expression in CD4(+) Th Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28848064] (Bioassay, Mouse) Bioassay Mouse
SK Varanasi, D Donohoe, U Jaggi, BT Rouse Manipulating Glucose Metabolism during Different Stages of Viral Pathogenesis Can Have either Detrimental or Beneficial Effects J. Immunol., 2017;0(0):. 2017 [PMID: 28768727] (Bioassay, Mouse) Bioassay Mouse
T Hosokawa, T Kimura, S Nada, T Okuno, D Ito, S Kang, S Nojima, K Yamashita, T Nakatani, Y Hayama, Y Kato, Y Kinehara, M Nishide, N Mikami, S Koyama, H Takamatsu, D Okuzaki, N Ohkura, S Sakaguchi, M Okada, A Kumanogoh Lamtor1 Is Critically Required for CD4(+) T Cell Proliferation and Regulatory T Cell Suppressive Function J. Immunol., 2017;0(0):. 2017 [PMID: 28768723] (Bioassay, Mouse) Bioassay Mouse
M Fernández, EM Monsalve, S López-Lópe, A Ruiz-Garcí, S Mellado, E Caminos, JJ García-Ram, J Laborda, P Tranque, MJM Díaz-Guerr Absence of Notch1 in murine myeloid cells attenuates the development of experimental autoimmune encephalomyelitis by affecting Th1 and Th17 priming Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 28762472] (Bioassay, Mouse) Bioassay Mouse
J Chen, JL Martindale, C Cramer, M Gorospe, U Atasoy, PD Drew, S Yu The RNA-binding protein HuR contributes to neuroinflammation by promoting C-C chemokine receptor 6 (CCR6) expression on Th17 cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28684423] (Bioassay, Mouse) Bioassay Mouse
J Ye, Y Wang, X Liu, L Li, A Opejin, EC Hsueh, H Luo, T Wang, D Hawiger, G Peng TLR7 Signaling Regulates Th17 Cells and Autoimmunity: Novel Potential for Autoimmune Therapy J. Immunol., 2017;0(0):. 2017 [PMID: 28652396] (Bioassay, Mouse) Bioassay Mouse
K Oh-Oka, Y Kojima, K Uchida, K Yoda, K Ishimaru, S Nakajima, J Hemmi, H Kano, Y Fujii-Kuri, R Katoh, H Ito, A Nakao Induction of Colonic Regulatory T Cells by Mesalamine by Activating the Aryl Hydrocarbon Receptor Cell Mol Gastroenterol Hepatol, 2017;4(1):135-151. 2017 [PMID: 28593185] (Bioassay, Mouse) Bioassay Mouse
B Afzali, J Grönholm, J Vandrovcov, C O'Brien, HW Sun, I Vanderleyd, FP Davis, A Khoder, Y Zhang, AN Hegazy, AV Villarino, IW Palmer, J Kaufman, NR Watts, M Kazemian, O Kamenyeva, J Keith, A Sayed, D Kasperavic, M Mueller, JD Hughes, IJ Fuss, MF Sadiyah, K Montgomery, J McElwee, NP Restifo, W Strober, MA Linterman, PT Wingfield, HH Uhlig, R Roychoudhu, TJ Aitman, P Kelleher, MJ Lenardo, JJ O'Shea, N Cooper, ADJ Laurence BACH2 immunodeficiency illustrates an association between super-enhancers and haploinsufficiency Nat. Immunol., 2017;0(0):. 2017 [PMID: 28530713] (Bioassay, Mouse) Bioassay Mouse
A Van Acker, K Gronke, A Biswas, L Martens, Y Saeys, J Filtjens, S Taveirne, E Van Ammel, T Kerre, P Matthys, T Taghon, B Vandekerck, J Plum, IR Dunay, A Diefenbach, G Leclercq A Murine Intestinal Intraepithelial NKp46-Negative Innate Lymphoid Cell Population Characterized by Group 1 Properties Cell Rep, 2017;19(7):1431-1443. 2017 [PMID: 28514662] (Bioassay, Mouse) Bioassay Mouse
J Geng, S Yu, H Zhao, X Sun, X Li, P Wang, X Xiong, L Hong, C Xie, J Gao, Y Shi, J Peng, RL Johnson, N Xiao, L Lu, J Han, D Zhou, L Chen The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells Nat. Immunol., 2017;0(0):. 2017 [PMID: 28504697] (Bioassay, Mouse) Bioassay Mouse
Show All 157 Publications.

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Bioinformatics

Gene Symbol Il12a
Entrez
Uniprot