Recombinant Mouse IL-12 Protein

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1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 25 kDa and 43 kDa (R) and 68 kDa (NR).
Recombinant Mouse IL-12 (Catalog # 419‑ML) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01‑0.1 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-12 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.01-0.1 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-12 protein
Mouse IL-12 p40
(Met23-Ser335)
Accession # P43432
Mouse IL-12 p35
(Arg23-Ala215)
Accession # NP_032377
N-terminus C-terminus
Accession #
N-terminal Sequence

Met23 (p40) & Arg23 (p35)

Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il12a
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

SDS-PAGE
70 kDa, non-reducing conditions
Publications
Read Publications using
419-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

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Publications for IL-12 (419-ML)(172)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Transgenic Mice.

We have publications tested in 5 applications: Bioassay, ELISA Standard, In Vivo, cell culture, celll culture.


Filter By Application
Bioassay
(137)
ELISA Standard
(8)
In Vivo
(22)
cell culture
(1)
celll culture
(1)
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Human
(1)
Mouse
(161)
Rat
(2)
N/A
(6)
Transgenic Mice
(1)
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Showing Publications 1 - 10 of 172. Show All 172 Publications.
Publications using 419-ML Applications Species
V Jelen?i?, M Šestan, I Kavazovi?, M Lenarti?, S Marinovi?, TD Holmes, M Prchal-Mur, B Lisni?, V Sexl, YT Bryceson, FM Wensveen, B Poli? NK cell receptor NKG2D sets activation threshold for the NCR1 receptor early in NK cell development Nat. Immunol., 2018;0(0):. 2018 [PMID: 30224819] (Bioassay, Mouse) Bioassay Mouse
HC Chuang, CY Tsai, CH Hsueh, TH Tan GLK-IKK? signaling induces dimerization and translocation of the AhR-ROR?t complex in IL-17A induction and autoimmune disease Sci Adv, 2018;4(9):eaat5401. 2018 [PMID: 30214937] (Bioassay, Mouse) Bioassay Mouse
A Martin, C Seignez, C Racoeur, N Isambert, Isambert N, Mabrouk N, N Mabrouk, A Scagliarin, Scagliarini A, Reveneau S, S Reveneau, L Arnould, Arnould L, A Bettaieb, JF Jeannin, Jeannin J, C Paul Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment. Oncotarget, 0;9(47):28364-28378. 0 [PMID: 29983866] (Bioassay, Rat) Bioassay Rat
M Zhao, MND Svensson, K Venken, A Chawla, S Liang, I Engel, P Mydel, J Day, D Elewaut, N Bottini, M Kronenberg Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70 Nat Commun, 2018;9(1):2627. 2018 [PMID: 29980684] (Bioassay, Mouse) Bioassay Mouse
P Garnier, R Mummery, MJ Forster, B Mulloy, RV Gibbs, CC Rider The localisation of the heparin binding sites of human and murine interleukin-12 within the carboxyterminal domain of the P40 subunit Cytokine, 2018;110(0):159-168. 2018 [PMID: 29753267] (Bioassay, Mouse) Bioassay Mouse
OAW Haabeth, M Fauskanger, M Manzke, KU Lundin, A Corthay, B Bogen, AA Tveita CD4+ T cell-mediated rejection of MHC class II-positive tumor cells is dependent on antigen secretion and indirect presentation on host APCs Cancer Res., 2018;0(0):. 2018 [PMID: 29752262] (Bioassay, Transgenic Mice) Bioassay Transgenic Mice
S Raju, K Kometani, T Kurosaki, AS Shaw, T Egawa The adaptor molecule CD2AP in CD4 T cells modulates differentiation of follicular helper T cells during chronic LCMV infection PLoS Pathog., 2018;14(5):e1007053. 2018 [PMID: 29734372] (Bioassay, Mouse) Bioassay Mouse
T Nabekura, Z Chen, C Schroeder, T Park, E Vivier, LL Lanier, D Liu Crk Adaptor Proteins Regulate NK Cell Expansion and Differentiation during Mouse Cytomegalovirus Infection J. Immunol., 2018;0(0):. 2018 [PMID: 29618525] (Bioassay, Mouse) Bioassay Mouse
N Andreas, M Riemann, CN Castro, M Groth, I Koliesnik, C Engelmann, T Sparwasser, T Kamradt, R Haenold, F Weih A new RelB-dependent CD117+CD172a+murine dendritic cell subset preferentially induces Th2 differentiation and supports airway hyperresponses in vivo Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29485182] (Bioassay, Mouse) Bioassay Mouse
SK Varanasi, U Jaggi, N Hay, BT Rouse Hexokinase II may be dispensable for CD4 T cell responses against a virus infection PLoS ONE, 2018;13(1):e0191533. 2018 [PMID: 29352298] (Bioassay, Mouse) Bioassay Mouse
Show All 172 Publications.

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