MRP1 Antibody (IU2H10) - BSA Free

Images

 
Orthogonal Strategies: Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; ...read more
Immunocytochemistry/ Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained ...read more
Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready, Dual ISH-IHC
Clone
IU2H10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

MRP1 Antibody (IU2H10) - BSA Free Summary

Immunogen
Recombinant peptide (a.a. 1-33) of human MRP1. [UniProt# P33527].
Epitope
Amino acids 8-17 of human MRP1.
Localization
Membrane
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ABCC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Dual RNAscope ISH-IHC
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
This MRP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band was observed at ~170-190 kDa in transfected samples. There may be a lower MW band that is a degradation product. Flow Cytometry, Immunoprecipitation and ELISA were reported in scientific literature. This antibody is CyTOF ready.
Publications
Read Publications using
NB400-156 in the following applications:

  • 1 publication
  • 2 publications
  • 2 publications
  • IP
    1 publication
  • WB
    8 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MRP1 Antibody (IU2H10) - BSA Free

  • ABC29
  • ABCC
  • ABCC1
  • ATP-binding cassette sub-family C member 1
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 1
  • EC 3.6.3
  • EC 3.6.3.44
  • GS-X
  • Leukotriene C(4) transporter
  • LTC4 transporter
  • MRP1
  • MRP1DKFZp781G125
  • MRPDKFZp686N04233
  • multidrug resistance associated protein 1
  • multidrug resistance protein
  • multidrug resistance-associated protein 1
  • multiple drug resistance protein 1
  • multiple drug resistance-associated protein

Background

The MRP molecule, an integral membrane glycophosphoprotein belonging to the same superfamily of ATP-binding cassette transmembrane transporter proteins as P-glycoprotein, is overexpressed in many P-glycoprotein-negative, multidrug resistant cell lines and tumours. It is believed that the main function of MRP in drug resistance is that of a plasma membrane drug efflux pump.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MRP1 Antibody (NB400-156)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ELISA, FLOW, ICC/IF, IP, WB.


Filter By Application
ELISA
(1)
FLOW
(2)
ICC/IF
(2)
IP
(1)
WB
(8)
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Filter By Species
Human
(10)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB400-156 Applications Species
Zhang S, Chatterjee T, Godoy C et al. GPR56 drives colorectal tumor growth and promotes drug resistance through upregulation of MDR1 expression via a RhoA-mediated mechanism Mol. Cancer Res. 2019-08-23 [PMID: 31444231] (WB, Human) WB Human
Li K, Ouyang L, He M et al. IDH1 R132H mutation regulates glioma chemosensitivity through Nrf2 pathway. Oncotarget 2017-04-25 [PMID: 28427200] (WB, Human) WB Human
Koshkin V, Ailles LE, Liu G, Krylov SN. Metabolic suppression of a drug resistant subpopulation in cancer spheroid cells J. Cell. Biochem. 2015-06-06 [PMID: 26054050] (FLOW, Human) FLOW Human
Kurihara Y, Sawazumi T, Takeuchi T. Exploration of interactions between membrane proteins embedded in supported lipid bilayers and their antibodies by reflectometric interference spectroscopy-based sensing. Analyst. 2014-10-15 [PMID: 25270526] (Human)

Details:
MRP1 antibody used for detection of Human MRP1 vesicles via microfluidic reflectometric interference spectroscopy (RIfS)-based sensor.
Human
Chen YY, Lukka PB, Joseph WR et al. Selective cellular uptake and retention of SN 28049, a new DNA-binding topoisomerase II-directed antitumor agent. Cancer Chemother. Pharmacol. 2014-05-07 [PMID: 24801172] (ICC/IF, Mouse) ICC/IF Mouse
Tiwari AK, Zhang R, Gallo JM et al. Overlapping functions of ABC transporters in topotecan disposition as determined in gene knockout Mouse models. Mol Cancer Ther 2013-05-01 [PMID: 23635651] (WB, Mouse) WB Mouse
Chen Q, Yang Y, Li L, Zhang JT. The amino terminus of the human multidrug resistance transporter ABCC1 has a U-shaped folding with a gating function. J Biol Chem;281(41):31152-63. 2006-10-13 [PMID: 16914551]
Binyamin L, Assaraf YG, Reiter Y. Probing ATP-dependent conformational changes in the multidrug resistance protein 1 (MRP1/ABCC1) in live tumor cells with a novel recombinant single-chain Fv antibody targeted to the extracellular N-terminus. Int J Cancer;116(5):703-9. 2005-09-20 [PMID: 15838832]
Yang Y, Chen Q, Zhang JT. Structural and functional consequences of mutating cysteine residues in the amino terminus of human multidrug resistance-associated protein 1. J Biol Chem;277(46):44268-77. 2002-11-15 [PMID: 12235150] (FLOW, Human) FLOW Human
Chen Q, Yang Y, Liu Y, Han B, Zhang JT. Cytoplasmic retraction of the amino terminus of human multidrug resistance protein 1. Biochemistry;41(29):9052-62. 2002-07-23 [PMID: 12119019] (ELISA, ICC/IF, IP, WB, Human) ELISA, ICC/IF, IP, WB Human
Show All 16 Publications.

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Product General Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Array NB400-156

Bioinformatics Tool for MRP1 Antibody (NB400-156)

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Blogs on MRP1.

ABC Membrane transporters and the role of MRP1 in drug resistance
ATP-binding cassette (ABC) transporters, alongside ion channels and aquaporins, are ubiquitous membrane-bound proteins that move substrates across extra and intra cellular membranes.  Multidrug resistance-associated protein 1 (MRP1) is a member o...  Read full blog post.

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Bioinformatics

Gene Symbol ABCC1