MRP1 Antibody (IU2H10) - BSA Free

Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.

Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready, Dual ISH-IHC
• Clone: IU2H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MRP1 Antibody (IU2H10) - BSA Free Summary

• Immunogen: Recombinant peptide (a.a. 1-33) of human MRP1. [UniProt# P33527].
• Epitope: Amino acids 8-17 of human MRP1.
• Localization: Membrane
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: This MRP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band was observed at ~170-190 kDa in transfected samples. There may be a lower MW band that is a degradation product. Flow Cytometry, Immunoprecipitation and ELISA were reported in scientific literature. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for MRP1 Antibody (IU2H10) - BSA Free

• ABC29
• ABCC
• ABCC1
• ATP-binding cassette sub-family C member 1
• ATP-binding cassette, sub-family C (CFTR/MRP), member 1
• EC 3.6.3
• EC 3.6.3.44
• GS-X
• Leukotriene C(4) transporter
• LTC4 transporter
• MRP1
• MRP1DKFZp781G125
• MRPDKFZp686N04233
• multidrug resistance associated protein 1
• multidrug resistance protein
• multidrug resistance-associated protein 1
• multiple drug resistance protein 1
• multiple drug resistance-associated protein

Background

The MRP molecule, an integral membrane glycophosphoprotein belonging to the same superfamily of ATP-binding cassette transmembrane transporter proteins as P-glycoprotein, is overexpressed in many P-glycoprotein-negative, multidrug resistant cell lines and tumours. It is believed that the main function of MRP in drug resistance is that of a plasma membrane drug efflux pump.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRP1 Antibody (NB400-156)(16)

Publications using NB400-156

• Zhang S, Chatterjee T, Godoy C et al. GPR56 drives colorectal tumor growth and promotes drug resistance through upregulation of MDR1 expression via a RhoA-mediated mechanism Mol. Cancer Res. 2019-08-23 [PMID: 31444231] (WB, Human)
• Li K, Ouyang L, He M et al. IDH1 R132H mutation regulates glioma chemosensitivity through Nrf2 pathway. Oncotarget 2017-04-25 [PMID: 28427200] (WB, Human)
• Koshkin V, Ailles LE, Liu G, Krylov SN. Metabolic suppression of a drug resistant subpopulation in cancer spheroid cells J. Cell. Biochem. 2015-06-06 [PMID: 26054050] (FLOW, Human)
• Kurihara Y, Sawazumi T, Takeuchi T. Exploration of interactions between membrane proteins embedded in supported lipid bilayers and their antibodies by reflectometric interference spectroscopy-based sensing. Analyst. 2014-10-15 [PMID: 25270526] (Human)
  
  Details:
  MRP1 antibody used for detection of Human MRP1 vesicles via microfluidic reflectometric interference spectroscopy (RIfS)-based sensor.
• Chen YY, Lukka PB, Joseph WR et al. Selective cellular uptake and retention of SN 28049, a new DNA-binding topoisomerase II-directed antitumor agent. Cancer Chemother. Pharmacol. 2014-05-07 [PMID: 24801172] (ICC/IF, Mouse)
• Tiwari AK, Zhang R, Gallo JM et al. Overlapping functions of ABC transporters in topotecan disposition as determined in gene knockout Mouse models. Mol Cancer Ther 2013-05-01 [PMID: 23635651] (WB, Mouse)
• Chen Q, Yang Y, Li L, Zhang JT. The amino terminus of the human multidrug resistance transporter ABCC1 has a U-shaped folding with a gating function. J Biol Chem;281(41):31152-63. 2006-10-13 [PMID: 16914551]
• Binyamin L, Assaraf YG, Reiter Y. Probing ATP-dependent conformational changes in the multidrug resistance protein 1 (MRP1/ABCC1) in live tumor cells with a novel recombinant single-chain Fv antibody targeted to the extracellular N-terminus. Int J Cancer;116(5):703-9. 2005-09-20 [PMID: 15838832]
• Yang Y, Chen Q, Zhang JT. Structural and functional consequences of mutating cysteine residues in the amino terminus of human multidrug resistance-associated protein 1. J Biol Chem;277(46):44268-77. 2002-11-15 [PMID: 12235150] (FLOW, Human)
• Chen Q, Yang Y, Liu Y, Han B, Zhang JT. Cytoplasmic retraction of the amino terminus of human multidrug resistance protein 1. Biochemistry;41(29):9052-62. 2002-07-23 [PMID: 12119019] (ELISA, ICC/IF, IP, WB, Human)
• Datta S, Sinha D EGCG maintained Nrf2-mediated redox homeostasis and minimized etoposide resistance in lung cancer cells Journal of Functional Foods 2019-11-01
• Datta S, Sinha D EGCG maintained Nrf2-mediated redox homeostasis and minimized EPE resistance in lung cancer cells Journal of Functional Foods 2019-11-01
• Bhadwal P, Randhawa V, Vaiphei K et al. Clinical relevance of CERK and SPHK1 in breast cancer and their association with metastasis and drug resistance Scientific reports 2022-10-29 [PMID: 36309544] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Huang R, Zhu L, Zhang Y XIST lost induces ovarian cancer stem cells to acquire taxol resistance via a KMT2C-dependent way Cancer cell international 2020-09-04 [PMID: 32943985] (WB, Human)
• Sridharan S, Robeson M, Bastihalli-Tukaramrao D, et al. Targeting of the Eukaryotic Translation Initiation Factor 4A Against Breast Cancer Stemness Front Oncol 2019-12-06 [PMID: 31867270] (WB, Human)
• Grybauskas A, Koga T, Kuprys PV et al. ABCB1 transporter and Toll-like receptor 4 in trabecular meshwork cells. Mol. Vis. 2015-03-24 [PMID: 25802484] (WB, Human, Mouse, Rat)

Bioinformatics

• Gene Symbol: ABCC1