MRP1 Antibody (IU2H10)

Images

 
Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.
Immunocytochemistry/ Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, CyTOF-ready
Clone
IU2H10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MRP1 Antibody (IU2H10) Summary

Immunogen
Recombinant peptide (a.a. 1-33) of human MRP1. [UniProt# P33527].
Epitope
Amino acids 8-17 of human MRP1.
Localization
Membrane
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ABCC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunoprecipitation
  • CyTOF-ready
Application Notes
This MRP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band was observed at ~170-190 kDa in transfected samples. There may be a lower MW band that is a degredation product. Flow Cytometry, Immunoprecipitation and ELISA were reported in scientific literature.
Publications
Read Publications using
NB400-156 in the following applications:

  • 1 publication
  • 2 publications
  • 2 publications
  • IP
    1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MRP1 Antibody (IU2H10)

  • ABC29
  • ABCC
  • ABCC1
  • ATP-binding cassette sub-family C member 1
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 1
  • EC 3.6.3
  • EC 3.6.3.44
  • GS-X
  • Leukotriene C(4) transporter
  • LTC4 transporter
  • MRP1
  • MRP1DKFZp781G125
  • MRPDKFZp686N04233
  • multidrug resistance associated protein 1
  • multidrug resistance protein
  • multidrug resistance-associated protein 1
  • multiple drug resistance protein 1
  • multiple drug resistance-associated protein

Background

The MRP molecule, an integral membrane glycophosphoprotein belonging to the same superfamily of ATP-binding cassette transmembrane transporter proteins as P-glycoprotein, is overexpressed in many P-glycoprotein-negative, multidrug resistant cell lines and tumours. It is believed that the main function of MRP in drug resistance is that of a plasma membrane drug efflux pump.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-46466
Species: Hu
Applications: IHC
NBP1-42349
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-22124
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-13415
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-76670
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P
NBP2-37923
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-33560
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91642
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-1471
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
AF6278
Species: Hu, Mu
Applications: WB
NB100-94881
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-52506
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC, IF
NLS6792
Species: Hu, Mu, Po, Bt, Bv, Ca, Eq, Ha, Mk, Pm, Rb
Applications: IHC-P
NBP1-33002
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-19417
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB25031
Species: Hu
Applications: WB, IHC, IP
NBP1-42342
Species: Hu
Applications: WB, Flow, IHC, IHC-Fr
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP

Publications for MRP1 Antibody (NB400-156)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ELISA, FLOW, ICC/IF, IP, WB.


Filter By Application
ELISA
(1)
FLOW
(2)
ICC/IF
(2)
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(6)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB400-156 Applications Species
Li K, Ouyang L, He M et al. IDH1 R132H mutation regulates glioma chemosensitivity through Nrf2 pathway. Oncotarget Apr 25 2017 [PMID: 28427200] (WB, Human) WB Human
Koshkin V, Ailles LE, Liu G, Krylov SN. Metabolic suppression of a drug resistant subpopulation in cancer spheroid cells J. Cell. Biochem. 2015 Jun 06 [PMID: 26054050] (FLOW, Human) FLOW Human
Grybauskas A, Koga T, Kuprys PV et al. ABCB1 transporter and Toll-like receptor 4 in trabecular meshwork cells. Mol. Vis. 2015 Mar 24 [PMID: 25802484] (WB, Human, Mouse, Rat) WB Human, Mouse, Rat
Kurihara Y, Sawazumi T, Takeuchi T. Exploration of interactions between membrane proteins embedded in supported lipid bilayers and their antibodies by reflectometric interference spectroscopy-based sensing. Analyst. 2014 Oct 15 [PMID: 25270526] (Human)

Details:
MRP1 antibody used for detection of Human MRP1 vesicles via microfluidic reflectometric interference spectroscopy (RIfS)-based sensor.
Human
Chen YY, Lukka PB, Joseph WR et al. Selective cellular uptake and retention of SN 28049, a new DNA-binding topoisomerase II-directed antitumor agent. Cancer Chemother. Pharmacol. 2014 May 07 [PMID: 24801172] (ICC/IF, Mouse) ICC/IF Mouse
Tiwari AK, Zhang R, Gallo JM et al. Overlapping functions of ABC transporters in topotecan disposition as determined in gene knockout Mouse models. Mol Cancer Ther 2013 May 1 [PMID: 23635651] (WB, Mouse) WB Mouse
Chen Q, Yang Y, Li L, Zhang JT. The amino terminus of the human multidrug resistance transporter ABCC1 has a U-shaped folding with a gating function. J Biol Chem;281(41):31152-63. 2006 Oct 13. [PMID: 16914551]
Binyamin L, Assaraf YG, Reiter Y. Probing ATP-dependent conformational changes in the multidrug resistance protein 1 (MRP1/ABCC1) in live tumor cells with a novel recombinant single-chain Fv antibody targeted to the extracellular N-terminus. Int J Cancer;116(5):703-9. 2005 Sep 20. [PMID: 15838832]
Yang Y, Chen Q, Zhang JT. Structural and functional consequences of mutating cysteine residues in the amino terminus of human multidrug resistance-associated protein 1. J Biol Chem;277(46):44268-77. 2002 Nov 15. [PMID: 12235150] (FLOW, Human) FLOW Human
Chen Q, Yang Y, Liu Y, Han B, Zhang JT. Cytoplasmic retraction of the amino terminus of human multidrug resistance protein 1. Biochemistry;41(29):9052-62. 2002 Jul 23. [PMID: 12119019] (WB, ELISA, IP, ICC/IF, Human) WB, ELISA, IP, ICC/IF Human

Reviews for MRP1 Antibody (NB400-156) (0)

There are no reviews for MRP1 Antibody (NB400-156). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MRP1 Antibody (NB400-156) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-156AF405
Alexa Fluor 488 Labeled NB400-156AF488
Alexa Fluor 647 Labeled NB400-156AF647
Alexa Fluor 700 Labeled NB400-156AF700
Allophycocyanin Labeled NB400-156APC
Biotin Labeled NB400-156B
DyLight 350 Labeled NB400-156UV
DyLight 405 Labeled NB400-156V
DyLight 488 Labeled NB400-156G
DyLight 550 Labeled NB400-156R
DyLight 650 Labeled NB400-156C
DyLight 680 Labeled NB400-156FR
DyLight 755 Labeled NB400-156IR
FITC Labeled NB400-156F
HRP Labeled NB400-156H
PE Labeled NB400-156PE

Additional MRP1 Products

MRP1 NB400-156

Bioinformatics Tool for MRP1 Antibody (NB400-156)

Discover related pathways, diseases and genes to MRP1 Antibody (NB400-156). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MRP1 Antibody (NB400-156)

Discover more about diseases related to MRP1 Antibody (NB400-156).
 

Pathways for MRP1 Antibody (NB400-156)

View related products by pathway.

PTMs for MRP1 Antibody (NB400-156)

Learn more about PTMs related to MRP1 Antibody (NB400-156).
 

Research Areas for MRP1 Antibody (NB400-156)

Find related products by research area.

Blogs on MRP1.

ABC Membrane transporters and the role of MRP1 in drug resistance
ATP-binding cassette (ABC) transporters, alongside ion channels and aquaporins, are ubiquitous membrane-bound proteins that move substrates across extra and intra cellular membranes.  Multidrug resistance-associated protein 1 (MRP1) is a member o...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MRP1 Antibody (IU2H10) and receive a gift card or discount.

Bioinformatics

Gene Symbol ABCC1
Entrez