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MDR1/ABCB1 Antibody (SN06-42)

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Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat ...read more
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.
Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Mouse (B6) liver tissue. Fixed in ice-cold methanol for 10 minutes. Blocking in 10% serum and 1% BSA. Primary antibody at 5 ug/mL, overnight ...read more
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat ...read more
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat ...read more
Immunocytochemistry/ Immunofluorescence: Rabbit Monoclonal MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Human brain microvascular endothelial cells stained for ABCB1, red and ciliary MDR1/ABCB1 green and nuclear DAPI ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SN06-42
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MDR1/ABCB1 Antibody (SN06-42) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within Human MDR1/ABCB1 aa 373-730 / 1,280. (SwissProt: P08183 Human; SwissProt: P06795 Mouse; SwissProt: P43245 Rat)
Localization
Cell membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ABCB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 31527311).
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-67667 in the following applications:

Publications
Read Publication using NBP2-67667.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for MDR1/ABCB1 Antibody (SN06-42)

  • ABC20
  • ABCB1
  • ABCB1B
  • ATP-binding cassette sub-family B member 1
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1
  • CD243 antigen
  • CD243
  • CLCS
  • colchicin sensitivity
  • doxorubicin resistance
  • EC 3.6.3
  • EC 3.6.3.44
  • GP170
  • IBD13
  • MDR1
  • MDR1MGC163296
  • multidrug resistance protein 1
  • P-glycoprotein 1
  • P-gp
  • PGY1
  • PGY1P-GP

Background

MDR1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MDR1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDR1/ABCB1 Antibody (NBP2-67667)(1)

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Reviews for MDR1/ABCB1 Antibody (NBP2-67667) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse-B6.

Reviews using NBP2-67667:
Filter by Applications
ICC
(1)
IF-Freshly embedded-Frozen
(1)
All Applications
Filter by Species
Human
(1)
Mouse-B6
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry MDR1/ABCB1 NBP2-67667
Enlarge
5
reviewed by:
KARTHIKEYAN THIRUGNANAM
ICC Human 01/23/2024
View

Summary

ApplicationImmunocytochemistry
Sample Testedhuman brain microvascular endothelial cells
SpeciesHuman
LotH01029
Other MDR1/ABCB1 NBP2-67667
Enlarge
4
reviewed by:
Milan Prajapati
IF-Freshly embedded-Frozen Mouse-B6 07/19/2019
View

Summary

ApplicationOther
Sample TestedLiver
SpeciesMouse-B6
LotHL0319

Comments

CommentsFresh embedding
sections - fix in ice-cold methanol 10 mins
Blocking - 10% serum +1%BSA
Primary - 5 ug/mL - overnight incubation
Secondary - 1.5 hour incubation (Invitrogen A-11012, 2 ug/mL)
mounting using Vector Vectashield with DAPI

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MDR1/ABCB1 Antibody (NBP2-67667) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for MDR1/ABCB1 Antibody (NBP2-67667)

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Blogs on MDR1/ABCB1

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

KARTHIKEYAN THIRUGNANAM
01/23/2024
Application: ICC
Species: Human

Milan Prajapati
07/19/2019
Application: IF-Freshly embedded-Frozen
Species: Mouse-B6

Bioinformatics

Gene Symbol ABCB1