MDR1/ABCB1 Antibody (SN06-42)

Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.

Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Mouse (B6) liver tissue. Fixed in ice-cold methanol for 10 minutes. Blocking in 10% serum and 1% BSA. Primary antibody at 5 ug/mL, overnight incubation. Secondary antibody at 2 ug/mL for 1.5 hour (Invitrogen A-11012). Mounting with Vector Vectashield with DAPI. Image from a verified customer review.

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: SN06-42
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MDR1/ABCB1 Antibody (SN06-42) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human MDR1/ABCB1 aa 373-730 / 1,280. (SwissProt: P08183 Human; SwissProt: P06795 Mouse; SwissProt: P43245 Rat)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ABCB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 31527311).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MDR1/ABCB1 Antibody (SN06-42)

• ABC20
• ABCB1
• ABCB1B
• ATP-binding cassette sub-family B member 1
• ATP-binding cassette, sub-family B (MDR/TAP), member 1
• CD243 antigen
• CD243
• CLCS
• colchicin sensitivity
• doxorubicin resistance
• EC 3.6.3
• EC 3.6.3.44
• GP170
• IBD13
• MDR1
• MDR1MGC163296
• multidrug resistance protein 1
• P-glycoprotein 1
• P-gp
• PGY1
• PGY1P-GP

Background

MDR1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MDR1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MDR1/ABCB1 Antibody (NBP2-67667)(1)

Publication using NBP2-67667

• Mercadante CJ, Prajapati M, Conboy HL et al. Manganese transporter Slc30a10 controls physiological manganese excretion and toxicity J. Clin. Invest. [PMID: 31527311] (ICC/IF)

Review for MDR1/ABCB1 Antibody (NBP2-67667) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse-B6.

reviewed by: Milan Prajapati IF - Freshly embedded - Frozen Mouse-B6 07/19/2019

Summary

• Application: Other
• Sample Tested: Liver
• Species: Mouse-B6
• Lot: HL0319

Comments

• Comments: Fresh embedding
  sections - fix in ice-cold methanol 10 mins
  Blocking - 10% serum +1%BSA
  Primary - 5 ug/mL - overnight incubation
  Secondary - 1.5 hour incubation (Invitrogen A-11012, 2 ug/mL)
  mounting using Vector Vectashield with DAPI

Bioinformatics

• Gene Symbol: ABCB1