ROBO4 Antibody (274914) [Unconjugated]

Jurkat human acute T cell leukemia cell line transfected with (A) mouse ROBO4 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse ROBO4 Monoclonal Antibody (Catalog # MAB50041) followed by APC-conjugated Goat anti-Rat IgG secondary antibody Catalog # F0113. Quadrant markers were set based on Rat IgG2A Isotype Control (Catalog # MAB006, data not shown). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 274914
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

ROBO4 Antibody (274914) [Unconjugated] Summary

• Immunogen: Y3 rat myeloid cell line transfected with mouse ROBO4
  Leu28-Ser1012
  Accession # Q8C310
• Specificity: Detects mouse ROBO4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROBO4 Antibody (274914) [Unconjugated]

• ECSM4
• FLJ20798
• Magic roundabout
• MGC133352
• MGC133353
• MRB
• ROBO4
• roundabout homolog 4
• roundabout homolog 4, magic roundabout (Drosophila)

Background

Roundabout receptors are molecular guidance proteins that function by interaction with Slit proteins to regulate axon guidance, neuronal migration, and leukocyte chemotaxis. ROBO4 expression is restricted to the endothelium and may also play a role in angiogenesis and endothelial cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8C310()