RIPK3/RIP3 Antibody (1312D) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
1312D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 594

RIPK3/RIP3 Antibody (1312D) [Alexa Fluor® 594] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
RIPK3/RIP3 Antibody (1312D) [Alexa Fluor 594] was made to a partial synthetic peptide made to an internal portion of the human RIPK3/RIP3 protein (between amino acids 470-540). This seqeunce has 100% homology to isoform CRA_a and isoform CRA_b.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RIPK3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Theoretical MW
56.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RIPK3/RIP3 Antibody (1312D) [Alexa Fluor® 594]

  • EC 2.7.11.1
  • Receptor Interacting Serine/Threonine Kinase 3
  • Receptor-interacting protein 3
  • receptor-interacting serine/threonine-protein kinase 3
  • receptor-interacting serine-threonine kinase 3
  • RIP3
  • RIP-3
  • RIPK3
  • RIP-like protein kinase 3

Background

RIPK3, Receptor interacting serine/threonine kinase 3 or RIP-like protein kinase 3 (human RIPK3 isoform1 theoretical molecular weight 57kDa) is a cytosolic protein with an amino terminal active kinase domain. RIP kinases, RIPK1 and RIPK3 play a central role in the induction of necroptosis, a form of programmed and inflammatory cell death that is caspase-independent (1). Necroptosis is a form of programmed necrosis which is initiated through the activation of RIPK3 by various ligands such as Fas, LPS and TNF. Formation of the necrosome results from the interaction between RIPK1 and RIPK3 mediated through their RIP homotypic interaction motifs (RHIMs) (1, 2). Following necrosome formation, RIPK3 activation leads to the phosphorylation of mixed lineage kinase domain-like protein (MLKL) which induces its oligomerization and translocation to the cell membrane where it alters plasma membrane integrity (2). Loss of membrane integrity results in lytic cell death, release of damage associated molecular patterns (DAMPs) and inflammation (2, 3). Therefore, the interaction of RIPK3 with other RHIM proteins is necessary for the initiation of necroptosis, while the execution phase is dependent on the activation of MLKL (4).

RIPK3 has several phosphorylation sites that are required for its role in necroptosis. For example, serine204 in mouse, which is conserved in the human (serine199), is necessary for necroptosis while serine residues 232 and 227 are both required for RIPK3s interaction with MLKL (2). The core necroptosis proteins RIPK1/RIPK3 and MLKL are implicated in several disease states such as neurodegeneration, cardiovascular, hepatic and pulmonary disease (3).

References

1. Orozco, S., & Oberst, A. (2017). RIPK3 in cell death and inflammation: the good, the bad, and the ugly. Immunological Reviews. https://doi.org/10.1111/imr.12536

2. Dhuriya, Y. K., & Sharma, D. (2018). Necroptosis: A regulated inflammatory mode of cell death. Journal of Neuroinflammation. https://doi.org/10.1186/s12974-018-1235-0

3. Choi, M. E., Price, D. R., Ryter, S. W., & Choi, A. M. K. (2019). Necroptosis: A crucial pathogenic mediator of human disease. JCI Insight. https://doi.org/10.1172/jci.insight.128834

4. Lee, K.-H., & Kang, T.-B. (2019). The Molecular Links between Cell Death and Inflammasome. Cells. https://doi.org/10.3390/cells8091057

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RIPK3/RIP3 Antibody (NBP2-76799AF594). (Showing 1 - 3 of 3 FAQ).

  1. What research areas can this product be used in?
    • All RIPK3/RIP3 products can be used in: Cancer, Cell Biology, Immunology, Necroptosis, Protein Kinase, Signal Transduction.
  2. What is the theoretical molecular weight for your RIPK3/RIP3 antibodies?
    • This depends on the immunogen. We have one RIPK3/RIP3 antibody [NBP1-77299] that is raised against murine RIP3. The theoretical molecular weight for this product is 53 kDa. The rest of our RIPK3/RIP3 antibodies are developed against human RIP3 and have a theoretical molecular weight of 56.7 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-76799AF350
Alexa Fluor 405 NBP2-76799AF405
Alexa Fluor 488 NBP2-76799AF488
Alexa Fluor 532 NBP2-76799AF532
Alexa Fluor 594 NBP2-76799AF594
Alexa Fluor 647 NBP2-76799AF647
Alexa Fluor 700 NBP2-76799AF700
Alexa Fluor 750 NBP2-76799AF750
Biotin NBP2-76799B
DyLight 350 NBP2-76799UV
DyLight 405 NBP2-76799V
DyLight 488 NBP2-76799G
DyLight 550 NBP2-76799R
DyLight 594 NBP2-76799DL594
DyLight 650 NBP2-76799C
DyLight 680 NBP2-76799FR
DyLight 755 NBP2-76799IR
FITC NBP2-76799F
HRP NBP2-76799H
Janelia Fluor 549 NBP2-76799JF549
Janelia Fluor 646 NBP2-76799JF646

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Bioinformatics Tool for RIPK3/RIP3 Antibody (NBP2-76799AF594)

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Bioinformatics

Gene Symbol RIPK3