RHAMM/CD168 Antibody - BSA Free

Images

 
Western Blot: RHAMM/CD168 Antibody [NBP1-76538] - Analysis in rat stomach tissue lysate with antibody at 0.5 ug/mL.
Immunohistochemistry: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue.
Flow Cytometry: RHAMM/CD168 Antibody [NBP1-76538] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in ...read more
Immunofluorescence: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue with CD168 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

RHAMM/CD168 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM. The immunogen is located within amino acids 80 - 130 of RHAMM. Amino Acid Squence: ERGAQDRRIQDLETELEK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMMR
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5 ug/ml
Control Peptide
RHAMM/CD168 Peptide (NBP1-76538PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-76538 in the following applications:

Publications
Read Publication using
NBP1-76538 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27856308).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for RHAMM/CD168 Antibody - BSA Free

  • CD168 antigen
  • CD168
  • HMMR
  • hyaluronan mediated motility receptor
  • hyaluronan-mediated motility receptor (RHAMM)
  • IHABP
  • Intracellular hyaluronic acid-binding protein
  • MGC119494
  • Receptor for hyaluronan-mediated motility
  • RHAMM
  • RHAMMMGC119495

Background

The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RHAMM/CD168 Antibody (NBP1-76538)(1)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
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Filter By Species
Human
(1)
Porcine
(1)
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Review for RHAMM/CD168 Antibody (NBP1-76538) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-76538:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry RHAMM/CD168 NBP1-76538
Enlarge
5
reviewed by:
Verified Customer
Flow Mouse 01/25/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedHippocampal Cell Suspension
SpeciesMouse
Lot6189-1502

Comments

CommentsUsed antibody for FACS. 5 x 10^6 Mouse primary hippocampal cells suspended in complete neurosphere supplement media at 4' C. Cells are not permeabilized. Primary antibody used at 1:200 dilution for an hour on ice. Secondary goat antibody diluted to 1:2000 used. BD FACS Aria Fusion Sorter was used to analyze the sample. Excitation wavelength 488nm. Emission Filter 530/30. Events acquired ~70,000. Controls used were as follows: 1) No staining without Propidium Iodide. 2)No staining with Propidium Iodide. 3)Secondary only with Propidium Iodide. Rhamm only with Propidium Iodide.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RHAMM/CD168 Antibody (NBP1-76538) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for RHAMM/CD168 Antibody (NBP1-76538)

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Blogs on RHAMM/CD168

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Recent Reviews

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Verified Customer
01/25/2018
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol HMMR
Uniprot