Western Blot: RHAMM/CD168 Antibody [NBP1-76538] - Analysis in rat stomach tissue lysate with antibody at 0.5 ug/mL.
Immunohistochemistry: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue.
Flow Cytometry: RHAMM/CD168 Antibody [NBP1-76538] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in ...read more
Immunofluorescence: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue with CD168 antibody at 20 ug/mL.
Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM. The immunogen is located within amino acids 80 - 130 of RHAMM. Amino Acid Squence: ERGAQDRRIQDLETELEK
Peptide affinity purified
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Porcine reactivity reported in scientific literature (PMID: 27856308).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for RHAMM/CD168 Antibody - BSA Free
hyaluronan mediated motility receptor
hyaluronan-mediated motility receptor (RHAMM)
Intracellular hyaluronic acid-binding protein
Receptor for hyaluronan-mediated motility
The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Used antibody for FACS. 5 x 10^6 Mouse primary hippocampal cells suspended in complete neurosphere supplement media at 4' C. Cells are not permeabilized. Primary antibody used at 1:200 dilution for an hour on ice. Secondary goat antibody diluted to 1:2000 used. BD FACS Aria Fusion Sorter was used to analyze the sample. Excitation wavelength 488nm. Emission Filter 530/30. Events acquired ~70,000. Controls used were as follows: 1) No staining without Propidium Iodide. 2)No staining with Propidium Iodide. 3)Secondary only with Propidium Iodide. Rhamm only with Propidium Iodide.
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