CD1d Antibody (703335)

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD1d Monoclonal Antibody (Catalog # MAB6979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD1d at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 703335
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD1d Antibody (703335) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD1d
  Glu20-Ser301 (predicted)
  Accession # P15813
• Specificity: Detects human CD1d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1c, rhCD1e, recombinant mouse (rm) CD1d1, and rmCD1d2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD1D
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD1d Antibody (703335)

• antigen-presenting glycoprotein CD1d
• CD1A
• CD1d antigen
• CD1D antigen, d polypeptide
• CD1d molecule
• CD1d
• differentiation antigen CD1-alpha-3
• HMC class I antigen-like glycoprotein CD1D
• MGC34622
• R3
• R3G1
• T-cell surface glycoprotein CD1d
• thymocyte antigen CD1D

Background

CD1d is a 48 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. Mature human CD1d consists of a 282 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within aa 19-300 (the ECD), human CD1d shares 65% and 68% aa sequence identity with mouse and rat CD1d, respectively. Complexes of CD1d with beta 2-microglobulin and endogenous glycolipids are constitutively expressed on antigen presenting cells, cortical thymocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatocytes (1). CD1d-presented glycolipids are recognized by canonical NKT cells that utilize an invariant mouse Va14-Ja18 chain in their T cell receptor (Va24-Ja18 in human) (2, 3). The interaction with glycolipid-loaded CD1d is critical for NKT cell development and induces their rapid secretion of both Th1 and Th2 type cytokines (3-6). In humans, infection with HSV-1 suppresses NKT cell activation by blocking the intracellular cycling of CD1d in antigen presenting cells (7).

1. Bendelac, A. et al. (2007) Annu. Rev. Immunol. 25:297.
2. Kawano, T. et al. (1997) Science 278:1626.
3. Chiu, Y.H. et al. (1999) J. Exp. Med. 189:103.
4. Behar, S.M. et al. (1999) J. Immunol. 162:161.
5. Mendiratta, S.K. et al. (1997) Immunity 6:469.
6. Stanic, A.K. et al. (2003) J. Immunol. 171:4539.
7. Yuan, W. et al. (2006) Nat. Immunol. 7:835.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD1d Antibody (MAB6979). (Showing 1 - 1 of 1 FAQ).

1. Do you have an antibody for CD1d that works well on formalin fixed paraffin embedded tissue (specifically for liver tissue)?
   • We do have a CD1d antibody that has been verified in paraffin-embedded tissue sections. The catalog number is NBP1-43461.

Bioinformatics

• Gene Symbol: CD1D
• Uniprot:
  • P15813()