CD1d Antibody (703335) Summary
Immunogen |
Mouse myeloma cell line NS0-derived recombinant human CD1d Glu20-Ser301 (predicted) Accession # P15813 |
Specificity |
Detects human CD1d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1c, rhCD1e, recombinant mouse (rm) CD1d1, and rmCD1d2 is observed. |
Source |
N/A |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
CD1D |
Purity |
Protein A or G purified from hybridoma culture supernatant |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
Protein A or G purified from hybridoma culture supernatant |
Reconstitution Instructions |
Sterile PBS to a final concentration of 0.5 mg/mL. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CD1d Antibody (703335)
Background
CD1d is a 48 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. Mature human CD1d consists of a 282 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within aa 19-300 (the ECD), human CD1d shares 65% and 68% aa sequence identity with mouse and rat CD1d, respectively. Complexes of CD1d with beta 2-microglobulin and endogenous glycolipids are constitutively expressed on antigen presenting cells, cortical thymocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatocytes (1). CD1d-presented glycolipids are recognized by canonical NKT cells that utilize an invariant mouse Va14-Ja18 chain in their T cell receptor (Va24-Ja18 in human) (2, 3). The interaction with glycolipid-loaded CD1d is critical for NKT cell development and induces their rapid secretion of both Th1 and Th2 type cytokines (3-6). In humans, infection with HSV-1 suppresses NKT cell activation by blocking the intracellular cycling of CD1d in antigen presenting cells (7).
- Bendelac, A. et al. (2007) Annu. Rev. Immunol. 25:297.
- Kawano, T. et al. (1997) Science 278:1626.
- Chiu, Y.H. et al. (1999) J. Exp. Med. 189:103.
- Behar, S.M. et al. (1999) J. Immunol. 162:161.
- Mendiratta, S.K. et al. (1997) Immunity 6:469.
- Stanic, A.K. et al. (2003) J. Immunol. 171:4539.
- Yuan, W. et al. (2006) Nat. Immunol. 7:835.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for CD1d Antibody (MAB6979). (Showing 1 - 1 of 1 FAQ).
-
Do you have an antibody for CD1d that works well on formalin fixed paraffin embedded tissue (specifically for liver tissue)?
- We do have a CD1d antibody that has been verified in paraffin-embedded tissue sections. The catalog number is NBP1-43461.
Secondary Antibodies
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Isotype Controls
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