CD1d Antibody (703335)

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Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD1d Monoclonal Antibody (Catalog # MAB6979) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
703335
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CD1d Antibody (703335) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD1d
Glu20-Ser301 (predicted)
Accession # P15813
Specificity
Detects human CD1d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1c, rhCD1e, recombinant mouse (rm) CD1d1, and rmCD1d2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD1D
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD1d Antibody (703335)

  • antigen-presenting glycoprotein CD1d
  • CD1A
  • CD1d antigen
  • CD1D antigen, d polypeptide
  • CD1d molecule
  • CD1d
  • differentiation antigen CD1-alpha-3
  • HMC class I antigen-like glycoprotein CD1D
  • MGC34622
  • R3
  • R3G1
  • T-cell surface glycoprotein CD1d
  • thymocyte antigen CD1D

Background

CD1d is a 48 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. Mature human CD1d consists of a 282 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within aa 19-300 (the ECD), human CD1d shares 65% and 68% aa sequence identity with mouse and rat CD1d, respectively. Complexes of CD1d with beta 2-microglobulin and endogenous glycolipids are constitutively expressed on antigen presenting cells, cortical thymocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatocytes (1). CD1d-presented glycolipids are recognized by canonical NKT cells that utilize an invariant mouse Va14-Ja18 chain in their T cell receptor (Va24-Ja18 in human) (2, 3). The interaction with glycolipid-loaded CD1d is critical for NKT cell development and induces their rapid secretion of both Th1 and Th2 type cytokines (3-6). In humans, infection with HSV-1 suppresses NKT cell activation by blocking the intracellular cycling of CD1d in antigen presenting cells (7).
  1. Bendelac, A. et al. (2007) Annu. Rev. Immunol. 25:297.
  2. Kawano, T. et al. (1997) Science 278:1626.
  3. Chiu, Y.H. et al. (1999) J. Exp. Med. 189:103.
  4. Behar, S.M. et al. (1999) J. Immunol. 162:161.
  5. Mendiratta, S.K. et al. (1997) Immunity 6:469.
  6. Stanic, A.K. et al. (2003) J. Immunol. 171:4539.
  7. Yuan, W. et al. (2006) Nat. Immunol. 7:835.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD1d Antibody (MAB6979). (Showing 1 - 1 of 1 FAQ).

  1. Do you have an antibody for CD1d that works well on formalin fixed paraffin embedded tissue (specifically for liver tissue)?
    • We do have a CD1d antibody that has been verified in paraffin-embedded tissue sections. The catalog number is NBP1-43461.

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Bioinformatics

Gene Symbol CD1D
Uniprot