RHAMM/CD168 Antibody [Alexa Fluor® 700]

Flow Cytometry: RHAMM/CD168 Antibody [Alexa Fluor® 700] [NBP1-76538AF700] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in 500 uL PBS/BSA/EDTA and viable DAPI cells were analysed using a Galios flow cytometer. Flow cytometry image submitted by a verified customer review.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 700

RHAMM/CD168 Antibody [Alexa Fluor® 700] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM. The immunogen is located within amino acids 80 - 130 of RHAMM.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMMR
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined. RHAMM/CD168 Antibody [Alexa Fluor(R) 700] validated for Flow cytometry from a verified customer review.
  
  Recommended applications are based on validated applications from the unconjugated base product NBP1-76538. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27856308).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50 mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RHAMM/CD168 Antibody [Alexa Fluor® 700]

• CD168 antigen
• CD168
• HMMR
• hyaluronan mediated motility receptor
• hyaluronan-mediated motility receptor (RHAMM)
• IHABP
• Intracellular hyaluronic acid-binding protein
• MGC119494
• Receptor for hyaluronan-mediated motility
• RHAMM
• RHAMMMGC119495

Background

The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RHAMM/CD168 Antibody (NBP1-76538AF700)(1)

Publication using NBP1-76538AF700

• Moyra L, Arash S, Susanne B et al. Mapping the biogenesis of forward programmed megakaryocytes from induced pluripotent stem cells Sciences Advances 2022-02-16 [PMID: 35171685]

Review for RHAMM/CD168 Antibody (NBP1-76538AF700) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Homo sapiens and Human.

reviewed by: Moyra Lawrence Flow Homo sapiens and Human 10/11/2019

Summary

• Application: Flow Cytometry
• Sample Tested: Megakaryocytes
• Species: Homo sapiens and Human
• Lot: 6189-1502-091619-AF700

Comments

• Comments: 47,000 CD41+CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20min at room temperature with the indicated quantity of antibody. Cells were then washed in 500uL PBS/BSA/EDTA and viable DAPI- cells were analysed using a Galios flow cytometer.

Bioinformatics

• Gene Symbol: HMMR