TPX2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-179] - Analysis of TPX2 at the mitotic spindle microtubules and poles in a HeLa metaphase cell. ICC/IF image submitted by a verified customer review.
Flow Cytometry: TPX2 Antibody [NB500-179] - RHAMM is not a cell surface but an intracellular cytoskeletal protein in mouse embryonic stem (ES) cells. (C) Alcohol permeabilized mouse ES cells, however, were strongly ...read more
Western Blot: TPX2 Antibody [NB500-179] - Analysis of TPX2 expression in 1) HeLa, 2) Ntera2, 3) K-562 and 4) Raji whole cell lysates using NB500-179.
Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-179] - Staining of HeLa cells fixed in 3.5% paraformaldehyde using NB 500-179 (1:1,000). Nuclear staining during interphase and spindle staining during ...read more
Simple Western: TPX2 Antibody [NB500-179] - Lane view shows a specific band for TPX2 using HeLa cell lysate and antibody at 1:250. Electropherogram image of corresponding Simple Western lane view. Image reported by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, MaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TPX2 Antibody Summary

Immunogen
A recombinant segment of the C-terminal domain of human TPX2 [UniProt# Q9ULW0]
Localization
Nuclear. Staining of non-mitotic cells and cell cycle-dependent changes in abundance peaking at G2/M phase.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPX2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:250
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 1:10-1:100
  • Knockdown Validated
Application Notes
This TPX2 antibody is useful for ICC/IF, Immunoprecipitation and Western Blot. In WB a band at approx. 100 kDa can be detected. Use in Flow Cytometry reported in scientific literature (PMID: 24019927). Knockdown validation (PMID: 31586073). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
TPX2 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB500-179 in the following applications:

Publications
Read Publications using
NB500-179 in the following applications:

  • 1 publication
  • 11 publications
  • IP
    1 publication
  • KD
    1 publication
  • WB
    18 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for TPX2 Antibody

  • C20orf1HCTP4
  • C20orf2
  • chromosome 20 open reading frame 1
  • Differentially expressed in cancerous and non-cancerous lung cells 2
  • differentially expressed in lung cells
  • DIL2
  • DIL-2FLS353
  • HCA519
  • Hepatocellular carcinoma-associated antigen 519
  • P100
  • p100GD:C20orf1
  • preferentially expressed in colorectal cancer
  • Protein fls353
  • REPP86
  • restricted expression proliferation associated protein 100
  • Restricted expression proliferation-associated protein 100
  • targeting protein for Xklp2
  • TPX2, microtubule-associated protein homolog
  • TPX2, microtubule-associated, homolog (Xenopus laevis)

Background

TPX2 (targeting protein for XKLP2) is an ortholog of the Targeting Protein for Xenopus kinesin-like protein 2 (XKLP2) and was originally named restricted expression/proliferation-associated protein 100 (REPP86). TPX2 regulates the Xenopus kinesin-like protein 2 which is a plus end-directed microtubule motor required for centrosome separation and maintenance of spindle polarity during mitosis. TPX2 levels steadily increase during cell cycle progression with highest expression levels in M phase and lowest levels in G1 phase. TPX2 activates mitotic serine-threonine Aurora A kinase, nucleates microtubules and is considered as a critical factor for spindle assembly in mammalian mitotic cells. It is actively transported into the nucleus during interphase via importin alpha/beta receptors and this nuclear import sequesters TPX2 away from cytoplasmic tubulin to prevent premature spindle assembly. TPX2 haploinsufficiency, leading to decreased levels of TPX2, significantly increases the susceptibility for tumor development in mice and cells deficient in the Aurora A/TPX2 complex presents short spindles which, despite of being able to segregate chromosomes, result in mitotic failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB500-179
Species: Hu, Mu, Rt, Po, Ma
Applications: WB, Simple Western, Flow, ICC/IF, IP, KD

Publications for TPX2 Antibody (NB500-179)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 5 applications: FLOW, ICC/IF, IP, KD, WB.


Filter By Application
FLOW
(1)
ICC/IF
(11)
IP
(1)
KD
(1)
WB
(18)
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Filter By Species
Human
(20)
Mouse
(7)
Porcine
(1)
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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB500-179 Applications Species
Naso FD, Sterbini V, Crecca E et al. Excess TPX2 Interferes with Microtubule Disassembly and Nuclei Reformation at Mitotic Exit Cells Feb 6 2020 [PMID: 32041138] (WB, Human) WB Human
Drutovic D, Duan X, Li R et al. RanGTP and importin beta regulate meiosis I spindle assembly and function in mouse oocytes EMBO J. 2019 Oct 16 [PMID: 31617608] (ICC/IF, Mouse) ICC/IF Mouse
Capalbo L, Bassi ZI, Geymonat M et al. The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis Nat Commun 2019 Oct 04 [PMID: 31586073] (WB, KD, Human) WB, KD Human
van Gijn SE, Wierenga E, van den Tempel N et al. TPX2/Aurora kinase A signaling as a potential therapeutic target in genomically unstable cancer cells. Oncogene Sep 3 2018 [PMID: 30177840] (WB, Human) WB Human
Mann BJ, Wadsworth P. Distribution of Eg5 and TPX2 in Mitosis: Insight from CRISPR tagged Cells Cytoskeleton (Hoboken) Aug 19 2018 [PMID: 30123975] (WB, ICC/IF, Human) WB, ICC/IF Human
Hsu WH, Wang WJ, Lin WY et al. Adducin-1 is essential for spindle pole integrity through its interaction with TPX2 EMBO Rep. Jun 19 2018 [PMID: 29925526] (WB, Human) WB Human
Manukyan A, Sargsyan L, Parsons SJ, Stukenberg PT. P190RhoGAP prevents mitotic spindle fragmentation and is required to activate Aurora A kinase at acentriolar poles Chromosoma 2018 Apr 14 [PMID: 29656322]
Park J, Kwon MS, Kim EE, Lee H. USP35 regulates mitotic progression by modulating the stability of Aurora B. Nat Commun. 2018 Feb 15 [PMID: 29449677] (WB, Human) WB Human
Connell M, Chen H, Jiang J et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development Elife. 2017 Oct 10 [PMID: 28994651] (Mouse) Mouse
Mann BJ, Balchand SK, Wadsworth P. Regulation of Kif15 localization and motility by the C-terminus of TPX2 and microtubule dynamics. Mol. Biol. Cell. Nov 16 2016 [PMID: 27852894]
Show All 33 Publications.

Review for TPX2 Antibody (NB500-179) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-179:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence TPX2 NB500-179
Enlarge
5
reviewed by:
Patrizia Lavia
IF Human 01/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PDF
SpeciesHuman
FileView PDF

Blocking

Blocking DetailsSee PDF

Details

Detection NotesSee PDF

Comments

CommentsNames and affiliations of the contributors:Lia Asteriti, Alessandra Russo, Giulia Guarguaglini an Patrizia Lavia, Institute of Molecular Biology and Pathology CNR, Rome, Italy

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TPX2 Antibody (NB500-179). (Showing 1 - 2 of 2 FAQs).

  1. In what assays cross-reactivity of NB500-179 with mouse has been confirmed? Do you have any information, or are there by chance any mouse images that just haven't made it onto the datasheet yet?
    • We tested this ab on mouse cells in a Western blot with good results.
  2. We know that the immunogen is a recombinant segment of the C-terminal domain of human TPX2. If it is possible, we would be interested to know the exact epitope within this domain.
    • Unfortunately, the exact immunogen sequence for NB500-179 is considered proprietary. It comes from the last 100 amino acids of TPX2.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

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Patrizia Lavia
01/16/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol TPX2
Entrez
Uniprot