TPX2 Antibody

Images

 
Western Blot: TPX2 Antibody [NB500-179] - Analysis of TPX2 expression in 1) HeLa, 2) Ntera2, 3) K-562 and 4) Raji whole cell lysates using NB500-179.
Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-179] - analysis of TPX2 at the mitotic spindle microtubules and poles in a HeLa metaphase cell. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-179] - Staining of HeLa cells fixed in 3.5% paraformaldehyde using NB 500-179 (1:1,000). Nuclear staining during interphase and spindle staining during ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, MaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TPX2 Antibody Summary

Immunogen
A recombinant segment of the C-terminal domain of human TPX2 [UniProt# Q9ULW0]
Localization
Nuclear. Staining of non-mitotic cells and cell cycle-dependent changes in abundance peaking at G2/M phase.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPX2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:250
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 1:10-1:100
Application Notes
This TPX2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot. In WB a band at approx. 100 kDa can be detected. Use in Flow Cytometry reported in scientific literature (PMID 24019927)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
TPX2 Lysate (NBL1-17236)
Reviewed Applications
Read 1 Review rated 5
using
NB500-179 in the following applications:

Publications
Read Publications using
NB500-179 in the following applications:

  • 1 publication
  • 9 publications
  • IP
    1 publication
  • WB
    10 publications

Reactivity Notes

Human, mammal, mouse, rat and pig.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for TPX2 Antibody

  • C20orf1HCTP4
  • C20orf2
  • chromosome 20 open reading frame 1
  • Differentially expressed in cancerous and non-cancerous lung cells 2
  • differentially expressed in lung cells
  • DIL2
  • DIL-2FLS353
  • HCA519
  • Hepatocellular carcinoma-associated antigen 519
  • P100
  • p100GD:C20orf1
  • preferentially expressed in colorectal cancer
  • Protein fls353
  • REPP86
  • restricted expression proliferation associated protein 100
  • Restricted expression proliferation-associated protein 100
  • targeting protein for Xklp2
  • TPX2, microtubule-associated protein homolog
  • TPX2, microtubule-associated, homolog (Xenopus laevis)

Background

TPX2 (targeting protein for XKLP2) is an ortholog of the Targeting Protein for Xenopus kinesin-like protein 2 (XKLP2) and was originally named restricted expression/proliferation-associated protein 100 (REPP86). TPX2 regulates the Xenopus kinesin-like protein 2 which is a plus end-directed microtubule motor required for centrosome separation and maintenance of spindle polarity during mitosis. TPX2 levels steadily increase during cell cycle progression with highest expression levels in M phase and lowest levels in G1 phase. TPX2 activates mitotic serine-threonine Aurora A kinase, nucleates microtubules and is considered as a critical factor for spindle assembly in mammalian mitotic cells. It is actively transported into the nucleus during interphase via importin alpha/beta receptors and this nuclear import sequesters TPX2 away from cytoplasmic tubulin to prevent premature spindle assembly. TPX2 haploinsufficiency, leading to decreased levels of TPX2, significantly increases the susceptibility for tumor development in mice and cells deficient in the Aurora A/TPX2 complex presents short spindles which, despite of being able to segregate chromosomes, result in mitotic failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56583
Species: Hu
Applications: WB, Flow, IHC-P
NBP1-87760
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB110-40561
Species: Hu
Applications: WB, IP
H00002968-B01P
Species: Hu
Applications: WB, ICC/IF
NBP1-84675
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-88650
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-01345
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
H00057410-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA
AF2699
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
AF3264
Species: Hu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
MAB2698
Species: Hu
Applications: WB, IHC, ICC
NB100-616
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
NB600-225
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-02665
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, ICC
NBP1-91499
Species: Mu, Bv
Applications: WB, ICC/IF, IHC
NBP2-13883
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB500-179
Species: Hu, Mu, Rt, Po, Ma
Applications: WB, Simple Western, Flow, ICC/IF, IP

Publications for TPX2 Antibody (NB500-179)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 4 applications: FLOW, ICC/IF, IP, WB.


Filter By Application
FLOW
(1)
ICC/IF
(9)
IP
(1)
WB
(10)
All Applications
Filter By Species
Human
(13)
Mouse
(5)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB500-179 Applications Species
Park J, Kwon MS, Kim EE, Lee H. USP35 regulates mitotic progression by modulating the stability of Aurora B. Nat Commun. 2018 Feb 15 [PMID: 29449677] (WB, Human) WB Human
Connell M, Chen H, Jiang J et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development Elife. 2017 Oct 10 [PMID: 28994651] (Mouse) Mouse
Mann BJ, Balchand SK, Wadsworth P. Regulation of Kif15 localization and motility by the C-terminus of TPX2 and microtubule dynamics. Mol. Biol. Cell. Nov 16 2016 [PMID: 27852894]
Rao SR, Flores-Rodriguez N, Page SL et al. The clathrin-dependent spindle proteome. Mol. Cell Proteomics. May 12 2016 [PMID: 27174698] (ICC/IF, Human) ICC/IF Human
Franciosi F, Manandhar S, Conti M. FSH Regulates mRNA Translation in Mouse Oocytes and Promotes Developmental Competence. Endocrinology 2016 Feb [PMID: 26653334] (Mouse) Mouse
Winkler C, De Munter S, Van Dessel N et al. The selective inhibition of protein phosphatase-1 results in mitotic catastrophe and impaired tumor growth. J. Cell. Sci. 2015 Nov 05 [PMID: 26542020] (WB, Human) WB Human
Barr AR, Bakal C. A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly. Sci Rep 2015 [PMID: 26037491] (WB, Human) WB Human
Cornago M, Garcia-Alberich C, Blasco-Angulo N et al. Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe. Cell Death Dis. 2014 Oct 03 [PMID: 25275596] (WB, ICC/IF, Human)

Details:
TPX2 antibody used for ICC-IF on U251-MG cells in metaphase after HDAC inhibitor suberanilohydroxamic acid/SAHA treatment (Figure 7a). Antibody also used for WB on lysates of glioma cell lines U87, U251-MG, LN229 cells as well as human GBM primary cultures C55, C65, C48 and C60 treated or not with HDAC inhibitor SAHA and valproic acid/VPA (Figure 7b and c)
WB, ICC/IF Human
Meyer HJ, Rape M. Enhanced protein degradation by branched ubiquitin chains. Cell. 2014 May 08 [PMID: 24813613] (ICC/IF, Human) ICC/IF Human
Song L, Craney A, Rape M. Microtubule-dependent regulation of mitotic protein degradation. Mol. Cell 2014 Jan 27 [PMID: 24462202] (WB, Human) WB Human
Show All 22 Publications.

Review for TPX2 Antibody (NB500-179) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-179:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence TPX2 NB500-179
Enlarge
reviewed by:
Patrizia Lavia
IF Human 01/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PDF
SpeciesHuman
FileView PDF

Blocking

Blocking DetailsSee PDF

Details

Detection NotesSee PDF

Comments

CommentsNames and affiliations of the contributors:Lia Asteriti, Alessandra Russo, Giulia Guarguaglini an Patrizia Lavia, Institute of Molecular Biology and Pathology CNR, Rome, Italy

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TPX2 Antibody (NB500-179). (Showing 1 - 2 of 2 FAQs).

  1. In what assays cross-reactivity of NB500-179 with mouse has been confirmed? Do you have any information, or are there by chance any mouse images that just haven't made it onto the datasheet yet?
    • We tested this ab on mouse cells in a Western blot with good results.
  2. We know that the immunogen is a recombinant segment of the C-terminal domain of human TPX2. If it is possible, we would be interested to know the exact epitope within this domain.
    • Unfortunately, the exact immunogen sequence for NB500-179 is considered proprietary. It comes from the last 100 amino acids of TPX2.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for TPX2 Antibody (NB500-179)

Discover related pathways, diseases and genes to TPX2 Antibody (NB500-179). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TPX2 Antibody (NB500-179)

Discover more about diseases related to TPX2 Antibody (NB500-179).
 

Pathways for TPX2 Antibody (NB500-179)

View related products by pathway.

PTMs for TPX2 Antibody (NB500-179)

Learn more about PTMs related to TPX2 Antibody (NB500-179).
 

Research Areas for TPX2 Antibody (NB500-179)

Find related products by research area.

Blogs on TPX2

There are no specific blogs for TPX2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Patrizia Lavia
01/16/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol TPX2
Entrez
Uniprot