| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76538-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52437-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38459-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56490-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26946 | |||
![Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-western-blot-119202416527..gif "<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody [NBP1-76538] - Analysis in rat stomach tissue lysate with antibody at 0.5 ug/mL.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Western-Blot-NBP1-76538-img0004.jpg)
![Immunohistochemistry: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-NBP1-76538-img0003.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody [NBP1-76538] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in 500 uL PBS/BSA/EDTA and viable DAPI cells were analysed using a Galios flow cytometer. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 700 version of this antibody.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Flow-Cytometry-NBP1-76538-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Immunofluorescence of RHAMM/CD168 in human stomach tissue with RHAM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-2711202420243358.jpg)
![Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against HEK293 (1) and CD168 (AA: 306-497)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-2F2C9-Western-Blot-NBP2-52437-img0003.jpg "Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against HEK293 (1) and CD168 (AA: 306-497)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on Jurkat cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437AF488 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-2F2C9-Flow-Cytometry-NBP2-52437-img0007.jpg)
![Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against human CD168 (AA: 306-497) recombinant protein. (Expected MW is 48.3 kDa)](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-2F2C9-Western-Blot-NBP2-52437-img0002.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis of Hela cells using CD168 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-2F2C9-Flow-Cytometry-NBP2-52437-img0001.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on A549 cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-2F2C9-Flow-Cytometry-NBP2-52437-img0005.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on Jurkat cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-2F2C9-Flow-Cytometry-NBP2-52437-img0006.jpg)
![ELISA: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-2F2C9-ELISA-NBP2-52437-img0004.jpg)
![Western Blot: CD168 Antibody [NBP2-15781] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CD168-Antibody-Western-Blot-NBP2-15781-img0001.jpg "Western Blot: CD168 Antibody [NBP2-15781] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-15781] - RHAMM/CD168 Antibody detects RHAMM/CD168 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RHAMM/CD168 Antibody dilution: 1:250. Scale bar = 100 um.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-15781-img0005.jpg)
![Immunohistochemistry-Paraffin: CD168 Antibody [NBP2-15781] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-15781-img0002.jpg)
![Immunoprecipitation: RHAMM/CD168 Antibody [NBP2-15781] - Immunoprecipitation of RHAMM protein from 293T whole cell extracts using 5 ug of RHAMM antibody [N1N2], N-term. Western blot analysis was performed using RHAMM antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunoprecipitation-NBP2-15781-img0004.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Analysis in human testis and prostate tissues using NBP2-38459 antibody. Corresponding HMMR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Analysis in human testis and prostate tissues using NBP2-38459 antibody. Corresponding HMMR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody [NBP2-38459] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Western-Blot-NBP2-38459-img0002.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0010.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Staining of human testis shows strong cytoplasmic/ membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0008.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Staining of human tonsil shows strong cytoplasmic/ membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0009.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-38459] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-Paraffin-NBP2-38459-img0011.jpg)
![Western Blot: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Western blot analysis of RHAMM/CD168 on different lysates with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SW480 cell lysate Lane 3: HepG2 cell lysate Lane 4: Raji cell lysate Lane 5: MCF7 cell lysate Lane 6: SK-Br-3 cell lysate Lane 7: HeLa cell lysate Lane 8: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-278202415212458.jpg "Western Blot: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Western blot analysis of RHAMM/CD168 on different lysates with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SW480 cell lysate Lane 3: HepG2 cell lysate Lane 4: Raji cell lysate Lane 5: MCF7 cell lysate Lane 6: SK-Br-3 cell lysate Lane 7: HeLa cell lysate Lane 8: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-29820241411547.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunocytochemistry analysis of HeLa cells labeling RHAMM/CD168 with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-2920241114816.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Flow cytometric analysis of HeLa cells labeling RHAMM/CD168. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32129, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-392024884629.jpg)
![Western Blot: RHAMM/CD168 Antibody (3L10H9) [NBP3-15424] - Western blot analysis of extracts of K-562 cells, using RHAMM/CD168 Rabbit mAb (NBP3-15424) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-3L10H9-Western-Blot-NBP3-15424-img0003.jpg "Western Blot: RHAMM/CD168 Antibody (3L10H9) [NBP3-15424] - Western blot analysis of extracts of K-562 cells, using RHAMM/CD168 Rabbit mAb (NBP3-15424) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody (3L10H9) [NBP3-15424] - Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] RHAMM/CD168 Rabbit mAb (NBP3-15424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-3L10H9-Immunohistochemistry-Paraffin-NBP3-15424-img0002.jpg)
![Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody (3L10H9) [NBP3-15424] - Immunohistochemistry of paraffin-embedded human esophageal using [KO Validated] RHAMM/CD168 Rabbit mAb (NBP3-15424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-3L10H9-Immunohistochemistry-Paraffin-NBP3-15424-img0001.jpg)
![Immunoprecipitation:[NBP3-30273]-Detection of human CD168/RHAMM by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CD168/RHAMM antibody used for IP at 6 µg per reaction. CD168/RHAMM was also immunoprecipitated by rabbit anti-CD168/RHAMM antibody. For blotting immunoprecipitated CD168/RHAMM, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30273_rabbit-rhamm-cd168-pab-23120251225622.jpg "Immunoprecipitation:[NBP3-30273]-Detection of human CD168/RHAMM by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CD168/RHAMM antibody used for IP at 6 µg per reaction. CD168/RHAMM was also immunoprecipitated by rabbit anti-CD168/RHAMM antibody. For blotting immunoprecipitated CD168/RHAMM, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: RHAMM/CD168 Antibody [NBP2-56490] - Staining of human cell line SiHa shows localization to microtubules. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56490-img0001.jpg "Immunocytochemistry/Immunofluorescence: RHAMM/CD168 Antibody [NBP2-56490] - Staining of human cell line SiHa shows localization to microtubules. Antibody staining is shown in green.")
![Western Blot: RHAMM/CD168 Antibody [NBP1-31797] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Western-Blot-NBP1-31797-img0003.jpg "Western Blot: RHAMM/CD168 Antibody [NBP1-31797] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: CD168 Overexpression Lysate (Adult Normal) [NBL1-11623] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD168.](http://images.novusbio.com/fullsize/CD168-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11623-img0002.jpg "Western Blot: CD168 Overexpression Lysate (Adult Normal) [NBL1-11623] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD168.")
![Western Blot: RHAMM/CD168 Antibody (S02-3E1) [NBP3-19597] - Western blot detection of RHAMM/CD168 in K562 cell lysates using NBP3-19597 (1:1000 diluted). Predicted band size: 84kDa. Observed band size: 84kDa.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-S02-3E1-Western-Blot-NBP3-19597-img0001.jpg "Western Blot: RHAMM/CD168 Antibody (S02-3E1) [NBP3-19597] - Western blot detection of RHAMM/CD168 in K562 cell lysates using NBP3-19597 (1:1000 diluted). Predicted band size: 84kDa. Observed band size: 84kDa.")
