RFX1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: RFX1 Antibody [NBP2-13223] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.

Immunohistochemistry-Paraffin: RFX1 Antibody [NBP2-13223] - Staining of human cerebral cortex shows nuclear positivity in neurons.

Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RFX1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RFX1 Antibody - BSA Free (NBP2-13223) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: SPLQLTNIQVPQQALPTQRLVVQSAAPGSKGGQVSLTVHGTQQVHSPPEQSPVQANSSSSKTAGAPTGTVPQQLQVHGVQQSVPVTQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFX1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RFX1 Protein (NBP2-13223PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RFX1 Antibody - BSA Free

• EFC
• EF-C
• Enhancer factor C
• MHC class II regulatory factor RFX
• MHC class II regulatory factor RFX1
• Regulatory factor X 1
• regulatory factor X, 1 (influences HLA class II expression)
• RFX
• trans-acting regulatory factor 1
• Transcription factor RFX1

Background

RFX1, also known as MHC class II regulatory factor RFX1, is a 105 kDa 979 amino acid protein found in the nucleus. The gene plays a significant role in the gene expression of MHC class II. Current research on RFX1 is being conducted in relation to hepatitis, leukemia, neuroblastoma , sickle cell anemia and adenocarcinoma. RFX1 has been linked to the methlation, immune response, spermatogenesis and localization pathways where it interacts with TADA3, SMAD1, HDAC1, ADD1 and SMAD9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFX1