RFX5 Antibody - BSA Free

RFX5-Antibody-Immunohistochemistry-NBP1-86041-img0011.jpg

Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Duodenum shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Kidney shows strong nuclear positivity in cells in tubules and in glomeruli.

Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Lymph node shows strong nuclear positivity in non-germinal center and germinal center cells.

nbp1-86041_rabbit-polyclonal-rfx5-antibody-20920231721151.jpg

Analysis in human cell line HDLM-2.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RFX5 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ARRLGGRGQSKYCYSGIRRKTLVSMPPLPGLDLKGSESPEMGPEVTPAPRDELVEAACALTCDWAERILKRSFSSIVEVARFLLQQHLISARSAHAHVLKAMGLAEEDEHAPRERSSKPKNGLENPEGGAHKKPERLAQPPKDLEART
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  RFX5 Protein (NBP1-86041PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RFX5 Antibody - BSA Free

• DNA-binding protein RFX5
• Regulatory factor X 5
• regulatory factor X, 5 (influences HLA class II expression)

Background

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RFX5 Antibody (NBP1-86041)(1)

Publication using NBP1-86041

• Chen DB, Xie XW, Zhao YJ et al. RFX5 promotes the progression of hepatocellular carcinoma through transcriptional activation of KDM4A Sci Rep 2020-09-03 [PMID: 32883983] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: RFX5