Home » RFX5 » RFX5 Antibodies » RFX5 Antibody

RFX5 Antibody

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: RFX5 Antibody [NBP1-86041] - TEST
Western Blot: RFX5 Antibody [NBP1-86041] - TEST
Immunohistochemistry: RFX5 Antibody [NBP1-86041] - The co-expression of RFX5 and KDM4A in HCC tissues was determined by the immunofluorescence staining. The white arrows pointed HCC cells which co-expressed RFX5 and ...read more
Western Blot: RFX5 Antibody [NBP1-86041] - Analysis in human cell line HDLM-2.
Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Kidney shows strong nuclear positivity in cells in tubules and in glomeruli.
Immunohistochemistry-Paraffin: RFX5 Antibody [NBP1-86041] - Staining of human Lymph node shows strong nuclear positivity in non-germinal center and germinal center cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

RFX5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ARRLGGRGQSKYCYSGIRRKTLVSMPPLPGLDLKGSESPEMGPEVTPAPRDELVEAACALTCDWAERILKRSFSSIVEVARFLLQQHLISARSAHAHVLKAMGLAEEDEHAPRERSSKPKNGLENPEGGAHKKPERLAQPPKDLEART
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RFX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
RFX5 Protein (NBP1-86041PEP)
Publications
Read Publication using
NBP1-86041 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RFX5 Antibody

  • DNA-binding protein RFX5
  • Regulatory factor X 5
  • regulatory factor X, 5 (influences HLA class II expression)

Background

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

RFX1 Antibody
NBP1-52654
Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: RFX1 Antibody [NBP1-52654] - Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for IP at 6 ug per reaction. RFX1 was also immunoprecipitated by another rabbit anti-RFX1 antibody. For blotting immunoprecipitated RFX1, NBP1-52654 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunohistochemistry-Paraffin: RFX1 Antibody [NBP1-52654] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
(4 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
CIITA Antibody -
BSA Free
NBP2-59072
Immunohistochemistry-Paraffin: CIITA Antibody [NBP2-59072] - Human brain tissue. Antibody concentration of 10 ug/ml.
Western Blot: CIITA Antibody [NBP2-59072] - Analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 ug/mL.
Immunocytochemistry/Immunofluorescence: CIITA Antibody [NBP2-59072] - Human brain tissue with CIITA antibody.
(2 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
RFXANK Antibody
(OTI3E7)
NBP2-01756
Flow Cytometry: RFXANK Antibody (OTI3E7) [NBP2-01756] - Analysis of Jurkat cells, using anti-RFXANK antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: RFXANK Antibody (OTI3E7) [NBP2-01756] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFXANK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFXANK.
Immunohistochemistry-Paraffin: RFXANK Antibody (OTI3E7) [NBP2-01756] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RFXANK mouse monoclonal antibody.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
RFXAP Antibody
H00005994-D01P
Immunocytochemistry/Immunofluorescence: RFXAP Antibody [H00005994-D01P] - Analysis of antibody to RFXAP on HeLa cell. Antibody concentration 20 ug/ml.
Western Blot: RFXAP Antibody [H00005994-D01P] - Analysis of RFXAP expression in transfected 293T cell line by RFXAP polyclonal antibody.Lane 1: RFXAP transfected lysate(28.30 KDa).Lane 2: Non-transfected lysate.
Western Blot: RFXAP Antibody [H00005994-D01P] - Analysis of RFXAP expression in human kidney.
Species: Hu
Applications: ICC/IF, WB
MHC Class I
Antibody (OX18) - BSA
NB120-6405
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY licence.
(33 Publications)
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
IFN-gamma
[Unconjugated]
285-IF
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma .
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
(2 Reviews)
(413 Publications)
Species: Hu
Applications: BA
CREB [p Ser133]
[Biotin]
DYC2510-2
A lysate prepared from HeLa human cervical epithelial carcinoma cells treated with 200 nM PMA for 20 minutes was analyzed with this DuoSet IC ELISA. The Human/Mouse/Rat Phospho-CREB (S133) DuoSet IC Detection Antibody was either untreated (no peptide) or pre-incubated with a phosphopeptide containing the CREB S133 phosphorylation site, a phosphopeptide containing the GSK-3 beta S9 phosphorylation site, a phosphopeptide containing the c-Jun S63 phosphorylation site, or a phosphopeptide containing the Raf1 S338 phosphorylation site. Peptides were used at 40 ng/mL. Only the phosphopeptide containing the CREB S133 phosphorylation site blocks the signal, indicating that the DuoSet IC ELISA is specific for CREB phosphorylation at S133.
Lysates prepared from HeLa human cervical epithelial carcinoma cells either uninduced or induced with 200 nM PMA for the indicated times were quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with either Anti-Human Phospho-CREB (S133) (Catalog # AF2510) or Anti-Human CREB (Catalog # AF2989) Polyclonal Antibodies. The DuoSet IC ELISA results correlate well with the amounts of phosphorylated CREB detected by Western blot. The immunoblot with anti-CREB antibody indicates that total levels of human CREB remained constant during incubation with PMA.The quantification of phosphorylated CREB with this DuoSet IC ELISA was also determined using cells pretreated with two concentrations of the MEK inhibitor U0126, which indirectly inhibits phosphorylation of CREB at S133.
HeLa human cervical epithelial carcinoma cells were untreated or treated with 200 nM PMA for 20 minutes, either with or without U0126 at the indicated concentrations. Cells were lysed and CREB phosphorylated at S133 was quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with either anti-phospho-CREB (S133) or anti-CREB antibodies. The DuoSet IC ELISA results correlate well with the amounts of phosphorylated CREB detected by Western blot. The immunoblot with anti-CREB antibody indicates that total levels of human CREB remained constant during the various treatments.The Phospho-CREB (S133) DuoSet IC ELISA also quantifies phosphorylated CREB levels in mouse and rat cell lysates.
(9 Publications)
Species: Hu, Mu, Rt
Applications: ELISA
Histone
Deacetylase 2/HDAC2 A
NBP1-87109
Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
COL1A2 Antibody
NBP2-92790
Western Blot: COL1A2 Antibody [NBP2-92790] - Analysis of extracts of A375 cells, using Collagen I/COL1A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: COL1A2 Antibody [NBP2-92790] - Human placenta using Collagen I/COL1A2 Rabbit pAb (A5786) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunocytochemistry/Immunofluorescence: COL1A2 Antibody [NBP2-92790] - Analysis of A-431 cells using Collagen I/COL1A2 antibody at dilution of 1:25. Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
HDAC9 Antibody -
BSA Free
NBP2-03993
Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
(1 Review)
Species: Bv, Ca, Hu, Mu, Pm
Applications: WB
RFX4 Antibody
NBP2-48967
Immunohistochemistry-Paraffin: RFX4 Antibody [NBP2-48967] - Staining in human testis and endometrium tissues using anti-RFX4 antibody. Corresponding RFX4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RFX4 Antibody [NBP2-48967] - Analysis in human testis and endometrium tissues. Corresponding RFX4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RFX4 Antibody [NBP2-48967] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
ANKRA2 Antibody
(1D11)
H00057763-M01
Immunocytochemistry/Immunofluorescence: ANKRA2 Antibody (1D11) [H00057763-M01] - Analysis of monoclonal antibody to ANKRA2 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: ANKRA2 Antibody (1D11) [H00057763-M01] - Detection limit for recombinant GST tagged ANKRA2 is approximately 0.03ng/ml as a capture antibody.
Western Blot: ANKRA2 Antibody (1D11) [H00057763-M01] - Analysis of ANKRA2 expression in transfected 293T cell line by ANKRA2 monoclonal antibody (M01), clone 1D11.Lane 1: ANKRA2 transfected lysate(34 KDa).Lane 2: Non-transfected lysate.
(1 Review)
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
HLA B Antibody
(2G7B10) - BSA Free
NBP2-61871
ELISA: HLA B Antibody (2G7B10) [NBP2-61871] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow Cytometry: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of MCF-7 cells using HLA-B mouse mAb (green) and negative control (red).
Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
HDAC1 Antibody
NB100-56340
Knockout Validated: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.
(2 Reviews)
(15 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
CBF2 Antibody
NBP2-47525
Immunohistochemistry: CBF2 Antibody [NBP2-47525] - Analysis of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Western Blot: CBF2 Antibody [NBP2-47525] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line NTERA-2
(2 Reviews)
Species: Hu
Applications: IHC, IHC-P, WB
NFYB Antibody
NBP2-19535
Immunocytochemistry/Immunofluorescence: NFYB Antibody [NBP2-19535] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYB protein stained by NFYB antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.
Immunoprecipitation: NFYB Antibody [NBP2-19535] - 293T whole cell lysate/extract A : 40 ug whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 ug of NFYB antibody 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) as used as a secondary reagent.
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Mouse intestine. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
PPAR gamma/NR1C3
Antibody - BSA Free
NBP2-22106
Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
(2 Reviews)
(8 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
HLA DPB1 Antibody
NBP1-89688
Immunohistochemistry-Paraffin: HLA DPB1 Antibody [NBP1-89688] - Staining in human lymph node and cerebral cortex tissues.. Corresponding HLA-DPB1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: HLA DPB1 Antibody [NBP1-89688] - Staining of human skin shows moderate to strong cytoplasmic positivity in epidermal cells.
Immunohistochemistry-Paraffin: HLA DPB1 Antibody [NBP1-89688] - Staining of human tonsil shows strong membranous positivity in germinal center cells.
Species: Hu
Applications: IHC, IHC-P, WB

Publications for RFX5 Antibody (NBP1-86041)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for RFX5 Antibody (NBP1-86041) (0)

There are no reviews for RFX5 Antibody (NBP1-86041). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RFX5 Antibody (NBP1-86041) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional RFX5 Products

Bioinformatics Tool for RFX5 Antibody (NBP1-86041)

Discover related pathways, diseases and genes to RFX5 Antibody (NBP1-86041). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RFX5 Antibody (NBP1-86041)

Discover more about diseases related to RFX5 Antibody (NBP1-86041).
 

Pathways for RFX5 Antibody (NBP1-86041)

View related products by pathway.

PTMs for RFX5 Antibody (NBP1-86041)

Learn more about PTMs related to RFX5 Antibody (NBP1-86041).
 

Research Areas for RFX5 Antibody (NBP1-86041)

Find related products by research area.

Blogs on RFX5

There are no specific blogs for RFX5, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RFX5 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol RFX5