HLA-DR Antibody (L243) - BSA Free


Orthogonal Strategies: Dual RNAscope ISH-IHC: HLA-DR Antibody (L243) [NB100-77855] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for HLA-DR mRNA (ACD RNAScope probe, ...read more
Western Blot: HLA-DR Antibody (L243) [NB100-77855] - Analysis of human spleen (A) and human tonsil (B) tissue using HLA-DR antibody at 2 ug/mL.
Immunohistochemistry-Paraffin: HLA-DR Antibody (L243) [NB100-77855] - Staining of HLA-DR in human spleen using DAB with hematoxylin counterstain.
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the FITC conjugate of NB100-77855. Staining of Human peripheral blood lymphocytes with L243 FITC.
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Human peripheral blood lymphocytes stained with L243 PE
Flow (Cell Surface): HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Azide Free version of NB100-77855. Staining of HLA-DR expression by 5x10^5 BDCM cells using 0.5 ug of (red) and 0.5 ug of isotype control ...read more
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the PerCP/Cy5.5 conjugate of NB100-77855. Staining of HLA-DR expression by 5x10^5 human PBMC using 10 uL (0.25 ug) of this antibody. Cell surface ...read more
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Alexa Fluor (R) 700 conjugate of NB100-77855. Staining of HLA-DR protein in human PBMCs using HLA-DR antibody [clone L243; conjugated to Alexa ...read more
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Biotin conjugate of NB100-77855. Staining of Human peripheral blood lymphocytes with biotinylated L243, followed by Sav-PE.
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the DyLight 405 conjugate of NB100-77855. Staining of HLA-DR in human PBMCs using anti-HLA-DR antibody. Image from verified customer review.

Product Details

Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, ELISA, EM, Flow, Flow-CS, Func, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, B/N, CyTOF-ready, Dual ISH-IHC
BSA Free
1.0 mg/ml
Validated by:

Orthogonal Strategies


Order Details

HLA-DR Antibody (L243) - BSA Free Summary

Human lymphoblastoid cell line (RPMI 8866).
IgG2a Kappa
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Block/Neutralize
  • CyTOF-ready
  • Dual RNAscope ISH-IHC
  • Electron Microscopy
  • Flow (Cell Surface)
  • Flow Cytometry < 0.5 ug/10^6 cells
  • Functional
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • Western Blot 1:100 - 1:2000
Application Notes
It is recommended that the reagent be titrated for optimal performance for each application. This antibody is CyTOF ready. Use in blocking/neutralizing reported in scientific literature (PMID: 12874328). Use in functional reported in scientific literature (PMID: 17900279). Use in ELISA reported in scientific literature (PMID: 11070170). Use in In vitro reported in scientific literature (PMID: 19620786). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 7835916). Use in electron microscopy reported in scientific literature (PMID: 21660937)
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NB100-77855 in the following applications:

Read Publications using
NB100-77855 in the following applications:

Reactivity Notes

Predicted cross-reactivity with Chimpanzee, Baboon, Cynomolgus, Rhesus, Marmoset, Tamarin, Squirrel Monkey and Canine.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein A purified

Alternate Names for HLA-DR Antibody (L243) - BSA Free

  • FLJ51114
  • histocompatibility antigen HLA-DR alpha
  • HLA class II histocompatibility antigen, DR alpha chain
  • HLA-DR
  • HLA-DRA1
  • major histocompatibility complex, class II, DR alpha
  • MHC cell surface glycoprotein
  • MHC class II antigen DRA
  • MHC Class II DR
  • MLRW


Human Leukocyte Antigen -DR isotype (HLA-DR) is a major histocompatibility complex (MHC) class II molecule expressed by antigen presenting cells (APCs) that plays a significant role in immune response (1). Class II molecules also include isoforms HLA-DP and -DQ (1,2). These type I membrane glycoproteins on APCs present peptides to helper T cells and T cell receptors on CD4+ cells (1). In humans, the genes encoding class II MHC proteins are located on chromosome 6p21, where HLA-DR is typically the most highly expressed, followed by HLA-DQ and then HLA-DP (3). Structurally, HLA-DR molecules are heterodimers consisting of an alpha chain subunit with an approximate theoretical molecular weight of 34 kDa and one of many approximately 30 kDa beta subunits (1-3). The alpha and beta genes are considered highly polymorphic with duplication resulting in nine DRB (beta subunit of HLA-DR) genes (DRB1-DRB9); though only DRB1, DRB3, DRB4, and DRB5 are considered functional (2,3). On the other hand, the alpha subunit is encoded by a single DRA gene (2,3). Studies focusing on the structural and biochemical properties of peptides that bind to HLA-DR molecules have helped contribute to subunit vaccine design and development (3).

Given the role in adaptive immunity, HLA-DR allele polymorphisms, gene misexpression, and dysfunction has been implicated in many diseases ranging from autoimmune disorders to cancer (2). HLA-DR is also a classical biomarker for disease, including sepsis where reduced expression of HLA-DR molecules on monocytes, as measured by flow cytometry, indicates diagnosis and prognosis (4,5). Immunosuppression observed with sepsis results in decreased surface expression of HLA-DR and concurrent increase in expression of programmed death 1 (PD-1), cytotoxic T-lymphocyte antigen 4 (CTLA-4), and B and T lymphocyte attenuator (BTLA) (4). This altered expression results in poor T cell response and apoptosis, along with reduced interferon-gamma (IFN-gamma) production and increased pro-inflammatory cytokine release (4). Furthermore, the decrease in HLA-DR expression is also correlated with the decrease in CD14lowCD16+ inflammatory monocytes (5). Interestingly, COVID-19 patients also exhibit a reduction in HLA-DR that correlates with disease severity and immunosuppression (5).


1. Andersson G. (1998). Evolution of the human HLA-DR region. Frontiers in bioscience : a journal and virtual library. https://doi.org/10.2741/a317

2. Shiina, T., Hosomichi, K., Inoko, H., & Kulski, J. K. (2009). The HLA genomic loci map: expression, interaction, diversity and disease. Journal of human genetics. https://doi.org/10.1038/jhg.2008.5

3. Stern, L. J., & Calvo-Calle, J. M. (2009). HLA-DR: molecular insights and vaccine design. Current pharmaceutical design. https://doi.org/10.2174/138161209789105171

4. Zhuang, Y., Peng, H., Chen, Y., Zhou, S., & Chen, Y. (2017). Dynamic monitoring of monocyte HLA-DR expression for the diagnosis, prognosis, and prediction of sepsis. Frontiers in bioscience (Landmark edition). https://doi.org/10.2741/4547

5. Benlyamani, I., Venet, F., Coudereau, R., Gossez, M., & Monneret, G. (2020). Monocyte HLA-DR Measurement by Flow Cytometry in COVID-19 Patients: An Interim Review. Cytometry. Part A : the journal of the International Society for Analytical Cytology. https://doi.org/10.1002/cyto.a.24249


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA-DR Antibody (NB100-77855)(30)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 12 applications: B/N, ELISA, EM, FLISA, FLOW, Func, ICC/F, IF/IHC, IHC-Fr, IP, In vitro, WB.

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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB100-77855 Applications Species
Bhargavan B, Woollard SM, McMillan JE, Kanmogne GD CCR5 antagonist reduces HIV-induced amyloidogenesis, tau pathology, neurodegeneration, and blood-brain barrier alterations in HIV-infected hu-PBL-NSG mice Molecular neurodegeneration 2021-11-22 [PMID: 34809709]
Mo Y, Cheung AKL, Liu Y et al. Imaging and analysis on the interaction between human antigen-pulsed V delta 2 T cells and antigen-specific CD4 T cells STAR Protocols 2021-06-01 [PMID: 33937873]
Liu L, Fang C, Fu W et al. Endothelial Cell-Derived IL-18 Released During Ischemia Reperfusion Injury Selectively Expands T Peripheral Helper Cells to Promote Alloantibody Production Circulation 2019-11-20 [PMID: 31744330]
Shibutani M, Maeda K, Nagahara H et al. A comparison of the local immune status between the primary and metastatic tumor in colorectal cancer: a retrospective study. BMC Cancer 2018-04-03 [PMID: 29614981] (IF/IHC, Human) IF/IHC Human
Petersen SH Odintsova E Haigh TA et al. The role of tetraspanin CD63 in antigen presentation via MHC class II. Eur J Immunol. 2011-09-01 [PMID: 21660937] (EM, Human) EM Human
Ivanov A Beers SA Walshe CA et al. Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells. J Clin Invest. 2009-08-01 [PMID: 19620786] (Func, In vitro, Human) Func, In vitro Human
Apps R Murphy SP Fernando R et al. Human leucocyte antigen (HLA) expression of primary trophoblast cells and placental cell lines, determined using single antigen beads to characterize allotype specificities of anti-HLA antibodies. Immunology. 2009-05-01 [PMID: 19368562] (FLOW, Human) FLOW Human
Wahlstrom J Dengjel J Persson B et al. Identification of HLA-DR-bound peptides presented by human bronchoalveolar lavage cells in sarcoidosis. J Clin Invest. 2007-11-01 [PMID: 17975675]
Setterblad N Garban F Weigl R et al. Extracorporeal photophoresis increases sensitivity of monocytes from patients with graft-versus-host disease to HLA-DR-mediated cell death. Transfusion 2008-01-01 [PMID: 17900279] (Func, Human)

HLA DRB1 antibody (clone L243) was used at 10ug/ml conncentration for induction of cell death ex vivo on peripheral blood mononuclear cells /PBMNCs that were isolated from graft-versus-host disease/GVHD patients.
Func Human
Dengjel J Schoor O Fischer R et al. Autophagy promotes MHC class II presentation of peptides from intracellular source proteins. Proc Natl Acad Sci U S A 2005-05-31 [PMID: 15894616]
Show All 30 Publications.

Review for HLA-DR Antibody (NB100-77855) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Canine.

Reviews using NB100-77855:
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Images Ratings Applications Species Date Details
Flow Cytometry HLA-DR NB100-77855
reviewed by:
Anushka George-Karote
Flow Canine 05/18/2018


ApplicationFlow Cytometry
Sample TestedWhole blood cells


CommentsThe whole blood cells were lysed to get rid of the RBC. The washed cell were then incubated with the Antibody. They were washed and then incubated with a conjugated secondary. Allowed to sit for 20mins and then washed again and read on the BD Fortessa.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HLA-DR Antibody (NB100-77855). (Showing 1 - 1 of 1 FAQs).

  1. We have an inquiry of your product Cat. ID # NB100-2735, NB600-1185, NB100-77758, NB100-78016, NB100-77855. We are interested in this product and would like to make sure that there is no any other protein added in the buffer. I saw the storage buffer in datasheet doesn't has any other protein but still would like to confirm with you again. Would you please help us to check and provide the purification method and buffer of the antibody?
    • Thank you for your inquiry. Catalog numbers NB100-77758, NB100-78016 and NB100-77855 are in a buffer containing PBS and sodium azide. NB600-1185 is in a buffer containing PBS, 150mM NaCl and sodium azide. NB100-2735 is whole tissue culture supernatant and may therefore contain other proteins.

Secondary Antibodies


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Recent Reviews


Anushka George-Karote
Application: Flow
Species: Canine


Gene Symbol HLA-DRA