HLA-DR Antibody (L243)

Images

 
Orthogonal Strategies: Dual RNAscope ISH-IHC: HLA-DR Antibody (L243) [NB100-77855] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for HLA-DR mRNA (ACD RNAScope probe, ...read more
Western Blot: HLA-DR Antibody (L243) [NB100-77855] - Analysis of human spleen (A) and human tonsil (B) tissue using HLA-DR antibody at 2 ug/mL.
Immunohistochemistry-Paraffin: HLA-DR Antibody (L243) [NB100-77855] - Staining of HLA-DR in human spleen using DAB with hematoxylin counterstain.
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the FITC conjugate of NB100-77855. Staining of Human peripheral blood lymphocytes with L243 FITC.
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Human peripheral blood lymphocytes stained with L243 PE
Flow (Cell Surface): HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Azide Free version of NB100-77855. Staining of HLA-DR expression by 5x10^5 BDCM cells using 0.5 ug of (red) and 0.5 ug of isotype control ...read more
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the PerCP/Cy5.5 conjugate of NB100-77855. Staining of HLA-DR expression by 5x10^5 human PBMC using 10 uL (0.25 ug) of this antibody. Cell surface ...read more
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Alexa Fluor (R) 700 conjugate of NB100-77855. Staining of HLA-DR protein in human PBMCs using HLA-DR antibody [clone L243; conjugated to Alexa ...read more
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Biotin conjugate of NB100-77855. Staining of Human peripheral blood lymphocytes with biotinylated L243, followed by Sav-PE.
Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the DyLight 405 conjugate of NB100-77855. Staining of HLA-DR in human PBMCs using anti-HLA-DR antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, ELISA, EM, Flow, Flow-CS, Func, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, B/N, CyTOF-ready, Dual ISH-IHC
Clone
L243
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

HLA-DR Antibody (L243) Summary

Immunogen
Human lymphoblastoid cell line (RPMI 8866).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-DRA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Electron Microscopy
  • ELISA
  • Flow Cytometry < 0.5 ug/10^6 cells
  • Functional
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • Block/Neutralize
  • CyTOF-ready
  • Dual RNAscope ISH-IHC
  • Flow (Cell Surface)
Application Notes
It is recommended that the reagent be titrated for optimal performance for each application. This antibody is CyTOF ready. Use in blocking/neutralizing reported in scientific literature (PMID: 12874328). Use in functional reported in scientific literature (PMID: 17900279). Use in ELISA reported in scientific literature (PMID: 11070170). Use in In vitro reported in scientific literature (PMID: 19620786). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 7835916). Use in electron microscopy reported in scientific literature (PMID: 21660937)
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-77855 in the following applications:

Publications
Read Publications using
NB100-77855 in the following applications:

Reactivity Notes

Predicted cross-reactivity with Chimpanzee, Baboon, Cynomolgus, Rhesus, Marmoset, Tamarin, Squirrel Monkey and Canine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for HLA-DR Antibody (L243)

  • FLJ51114
  • histocompatibility antigen HLA-DR alpha
  • HLA class II histocompatibility antigen, DR alpha chain
  • HLADR
  • HLA-DR
  • HLA-DRA
  • HLA-DRA1
  • major histocompatibility complex, class II, DR alpha
  • MHC cell surface glycoprotein
  • MHC class II antigen DRA
  • MHC Class II DR
  • MLRW

Background

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD alpha (heavy) chain and a 27 kD beta (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. L243 has been reported to block mixed lymphocyte reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA-DR Antibody (NB100-77855)(28)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 12 applications: B/N, ELISA, EM, FLISA, FLOW, Func, ICC-IF, IHC, IHC-Fr, IP, In vitro, WB.


Filter By Application
B/N
(3)
ELISA
(3)
EM
(1)
FLISA
(1)
FLOW
(11)
Func
(7)
ICC-IF
(1)
IHC
(1)
IHC-Fr
(2)
IP
(3)
In vitro
(2)
WB
(1)
All Applications
Filter By Species
Human
(20)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NB100-77855 Applications Species
Liu L, Fang C, Fu W et al. Endothelial Cell-Derived IL-18 Released During Ischemia Reperfusion Injury Selectively Expands T Peripheral Helper Cells to Promote Alloantibody Production Circulation Nov 20 2019 [PMID: 31744330]
Shibutani M, Maeda K, Nagahara H et al. A comparison of the local immune status between the primary and metastatic tumor in colorectal cancer: a retrospective study. BMC Cancer Apr 3 2018 [PMID: 29614981] (IHC, Human) IHC Human
Petersen SH Odintsova E Haigh TA et al. The role of tetraspanin CD63 in antigen presentation via MHC class II. Eur J Immunol. 2011 Sep 1 [PMID: 21660937] (EM, Human) EM Human
Ivanov A Beers SA Walshe CA et al. Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells. J Clin Invest. 2009 Aug 1 [PMID: 19620786] (Func, In vitro, Human) Func, In vitro Human
Apps R Murphy SP Fernando R et al. Human leucocyte antigen (HLA) expression of primary trophoblast cells and placental cell lines, determined using single antigen beads to characterize allotype specificities of anti-HLA antibodies. Immunology. 2009 May 1 [PMID: 19368562] (FLOW, Human) FLOW Human
Wahlstrom J Dengjel J Persson B et al. Identification of HLA-DR-bound peptides presented by human bronchoalveolar lavage cells in sarcoidosis. J Clin Invest. 2007 Nov 1 [PMID: 17975675]
Setterblad N Garban F Weigl R et al. Extracorporeal photophoresis increases sensitivity of monocytes from patients with graft-versus-host disease to HLA-DR-mediated cell death. Transfusion 2008 Jan 1 [PMID: 17900279] (Func, Human)

Details:
HLA DRB1 antibody (clone L243) was used at 10ug/ml conncentration for induction of cell death ex vivo on peripheral blood mononuclear cells /PBMNCs that were isolated from graft-versus-host disease/GVHD patients.
Func Human
Dengjel J Schoor O Fischer R et al. Autophagy promotes MHC class II presentation of peptides from intracellular source proteins. Proc Natl Acad Sci U S A 2005 May 31 [PMID: 15894616]
Ovsyannikova IG Johnson KL Naylor S Poland GA. Identification of HLA-DRB1-bound self-peptides following measles virus infection. J Immunol Methods. 2005 Feb 1 [PMID: 15777939] (FLOW, Human) FLOW Human
Pashine A Busch R Belmares MP et al. Interaction of HLA-DR with an acidic face of HLA-DM disrupts sequence-dependent interactions with peptides. Immunity 2003 Aug 1 [PMID: 12932352] (FLOW) FLOW
Show All 28 Publications.

Review for HLA-DR Antibody (NB100-77855) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Canine.

Reviews using NB100-77855:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Canine
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry HLA-DR NB100-77855
Enlarge
reviewed by:
Anonymous
Flow Canine 05/18/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedWhole blood cells
SpeciesCanine
LotA3

Comments

CommentsThe whole blood cells were lysed to get rid of the RBC. The washed cell were then incubated with the Antibody. They were washed and then incubated with a conjugated secondary. Allowed to sit for 20mins and then washed again and read on the BD Fortessa.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HLA-DR Antibody (NB100-77855). (Showing 1 - 1 of 1 FAQs).

  1. We have an inquiry of your product Cat. ID # NB100-2735, NB600-1185, NB100-77758, NB100-78016, NB100-77855. We are interested in this product and would like to make sure that there is no any other protein added in the buffer. I saw the storage buffer in datasheet doesn't has any other protein but still would like to confirm with you again. Would you please help us to check and provide the purification method and buffer of the antibody?
    • Thank you for your inquiry. Catalog numbers NB100-77758, NB100-78016 and NB100-77855 are in a buffer containing PBS and sodium azide. NB600-1185 is in a buffer containing PBS, 150mM NaCl and sodium azide. NB100-2735 is whole tissue culture supernatant and may therefore contain other proteins.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-77855AF350
Alexa Fluor 405 NB100-77855AF405
Alexa Fluor 488 NB100-77855AF488
Alexa Fluor 532 NB100-77855AF532
Alexa Fluor 594 NB100-77855AF594
Alexa Fluor 647 NB100-77855AF647
Alexa Fluor 700 NB100-77855AF700
Alexa Fluor 750 NB100-77855AF750
Allophycocyanin NB100-77855APC
Allophycocyanin/Cy7 NB100-77855APCCY7
Biotin NB100-77857
DyLight 350 NB100-77855UV
DyLight 405 NB100-77855V
DyLight 488 NB100-77855G
DyLight 594 NB100-77855DL594
DyLight 680 NB100-77855FR
DyLight 755 NB100-77855IR
FITC NB100-77856
Janelia Fluor 549 NB100-77855JF549
Janelia Fluor 646 NB100-77855JF646
PE NB100-77855PE
PE/Atto594 NB100-77855PEATT594
PE/Cy5.5 NB100-77855PECY55
PE/Cy7 NB100-77855PECY7
PerCP NB100-77855PCP

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Recent Reviews

5
5
1
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0
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2
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1
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Anonymous
05/18/2018
Application: Flow
Species: Canine

Bioinformatics

Gene Symbol HLA-DRA