CIITA Antibody - BSA Free

Western Blot: CIITA Antibody [NBP2-59072] - Analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 ug/mL.

Immunocytochemistry/Immunofluorescence: CIITA Antibody [NBP2-59072] - Human brain tissue with CIITA antibody.

Immunohistochemistry-Paraffin: CIITA Antibody [NBP2-59072] - Human brain tissue. Antibody concentration of 10 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CIITA Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CIITA. The immunogen is located within amino acids 120 - 170 of CIITA. Amino Acid Squence: AEVCQSQRRPFPEEL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIITA
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  CIITA Protein (NBP2-59072PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CIITA Antibody - BSA Free

• C2TA
• C2TAMHC class II transactivator type III
• CIITAIV
• class II, major histocompatibility complex, transactivator
• MHC class II transactivator
• MHC2TANLR family, acid domain containing
• NLRA
• nucleotide-binding oligomerization domain, leucine rich repeat and acid domaincontaining

Background

The transactivator CIITA regulates basal and interferon-induced expression of Major Histocompatibility Complex class II genes. CIITA restores expression of all MHC class II gene expression in mutant cells and corrects regulatory defects of MHC class II genes. Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression. Antigen can be obtained from Raji cell lysates. Typically levels of CIITA expression are too low to detect endogenous levels of protein expression. Transiently transfected cells are usually employed to study this transcription factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CIITA Antibody (NBP2-59072)(2)

Publications using NBP2-59072

• Jeong H, Lee H, Kim H et al. Cellular plasticity and immune microenvironment of malignant pleural effusion are associated with EGFR-TKI resistance in non-small-cell lung carcinoma iScience 2022-11-01 [PMID: 36339256] (ICC/IF, Human)
• Xue Y, Tang D, Li SJ et al. Link between CIITA rs3087456 polymorphism and the risk of laryngeal squamous cell carcinoma in a Chinese population Pathol. Res. Pract. 2019-12-18 [PMID: 31870593] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: CIITA