CIITA Antibody

Images

 
Western Blot: CIITA Antibody [NBP2-59072] - Analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: CIITA Antibody [NBP2-59072] - Human brain tissue with CIITA antibody.
Immunohistochemistry-Frozen: CIITA Antibody [NBP2-59072] - MHCII staining of human tonsil. Image from verified customer review.
Immunohistochemistry-Paraffin: CIITA Antibody [NBP2-59072] - Human brain tissue. Antibody concentration of 10 ug/ml.
Methylated DNA Immunoprecipitation: CIITA Antibody [NBP2-59072] - Arnalysis of MHC Class II in rat mesenchymal stem cells using anti-MHC Class II antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IHC-P, MeDIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CIITA Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CIITA. The immunogen is located within amino acids 120 - 170 of CIITA.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CIITA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Methylated DNA Immunoprecipitation 1:10-1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23918395)
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CIITA Protein (NBP2-59072PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CIITA Antibody

  • C2TA
  • C2TAMHC class II transactivator type III
  • CIITAIV
  • class II, major histocompatibility complex, transactivator
  • MHC class II transactivator
  • MHC2TANLR family, acid domain containing
  • NLRA
  • nucleotide-binding oligomerization domain, leucine rich repeat and acid domaincontaining

Background

The transactivator CIITA regulates basal and interferon-induced expression of Major Histocompatibility Complex class II genes. CIITA restores expression of all MHC class II gene expression in mutant cells and corrects regulatory defects of MHC class II genes. Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression. Antigen can be obtained from Raji cell lysates. Typically levels of CIITA expression are too low to detect endogenous levels of protein expression. Transiently transfected cells are usually employed to study this transcription factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CIITA