Reg3B Antibody

• Reactivity: Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Reg3B Antibody Summary

• Immunogen: E. coli-derived recombinant rat Reg3B
  Glu27-Gly175
  Accession # P25031
• Specificity: Detects rat Reg3B in direct ELISAs and Western blots. In direct ELISAs, approxmately 75% cross-reactivity with recombinant mouse (rm) Reg3B, and approximately 20% cross-reactivity with rmReg3G is observed, and less than 2% cross-reactivity with recombinant human (rh) Reg3A, rmReg1, rmReg2, rhReg4, rhReg1A, rmReg3A, and rmReg3D is observed. In Western blots, approximately 40% cross-reactivity with rmReg3B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Reg3b
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg3B Antibody

• PAP1
• Reg3B

Background

Rat Reg3B, also named pancreatitis-associated protein 1, Regenerating islet-derived protein 3-beta and peptide 23, belongs to the Regenerating gene family (Reg), within the superfamily of C-type lectins. It has been classified as a member of the type III subclass of the Reg family. Rat Reg3B has anti-apoptotic effects on pancreatic acinar cells and is a novel motor and sensory neuron survival factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Reg3B Antibody (AF1996)(5)

Publications using AF1996

• Huairong Zhang, Andrea Liliam Gomez Corredor, Julia Messina-Pacheco, Qing Li, George Zogopoulos, Nancy Kaddour et al. REG3A/REG3B promotes acinar to ductal metaplasia through binding to EXTL3 and activating the RAS-RAF-MEK-ERK signaling pathway Communications Biology 6/7/2021 [PMID: 34099862]
• J Li, H Shi, H Liu, F Dong, Z Liu, Y Lu, L Chen, L Bao, X Zhang Nerve Injury-Induced Neuronal PAP-I Maintains Neuropathic Pain by Activating Spinal Microglia J. Neurosci., 2019-11-19;40(2):297-310. 2019-11-19 [PMID: 31744864] (Western Blot, Human, Rat, Transgenic Rat)
• S Tremblay, G Romain, M Roux, XL Chen, K Brown, DL Gibson, S Ramanathan, A Menendez Bile acid administration elicits an intestinal antimicrobial program and reduces the bacterial burden in two mouse models of enteric infection Infect. Immun, 2017-05-23;0(0):. 5/23/2017 [PMID: 28348052]
• A SILAC-Based Method for Quantitative Proteomic Analysis of Intestinal Organoids Sci Rep, 2016-11-30;6(0):38195. 2016-11-30 [PMID: 27901089] (Western Blot, Mouse)
• van Ampting MT, Rodenburg W, Vink C, Kramer E, Schonewille AJ, Keijer J, van der Meer R, Bovee-Oudenhoven IM Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Infection in Rats. Dig. Dis. Sci., 2009-12-01;54(0):2588. 2009-12-01 [PMID: 19160051] (IHC-P, Rat)

Reviews for Reg3B Antibody (AF1996) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 01/06/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21410322
• Species: Mouse

reviewed by: Verified Customer WB Mouse 01/06/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23147030
• Species: Mouse

Bioinformatics

• Gene Symbol: Reg3b
• Uniprot:
  • P25031()