Recombinant Rat E-Cadherin Fc Chimera Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat E-Cadherin Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of P19 mouse embryonal carcinoma cells. The ED₅₀ for this effect is 0.25-1.25 μg/mL
• Source: Mouse myeloma cell line, NS0-derived rat E-Cadherin protein
  

  Rat E-Cadherin (Gln 24-Ser704) Accession # Q9R0T4	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q9R0T4
• N-terminal Sequence: Asp159 & No results obtained: Gln24 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cdh1
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 103 kDa (pro monomer) & 87 kDa (mature monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-125 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat E-Cadherin Fc Chimera Protein, CF

• Arc-1
• CAD1
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• Cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDH1
• CDHE
• cell-CAM 120/80
• Cell-CAM120/80
• ECAD
• ECadherin
• E-Cadherin
• Epithelial cadherin
• LCAM
• L-CAM
• UVOE-Cadherin
• Uvomorulin

Background

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Rat E-Cadherin shares 92% and 81% amino acid sequence identity with the mouse and human proteins, respectively (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGF R‑dependent survival and proliferation (6).

1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
2. Nagafuchi, A. et al. (1987) Nature 329:341.
3. Guillot, C. and T. Lecuit (2013) Science 340:1185.
4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.

Publications for E-Cadherin (8144-EC)(2)

Publications using 8144-EC

• YA Yang, E Nguyen, GHN Sankara Na, M Heuzé, C Fu, H Yu, RM Mège, B Ladoux, MP Sheetz Local contractions regulate E-cadherin rigidity sensing Science Advances, 2022-01-28;8(4):eabk0387. 2022-01-28 [PMID: 35089785] (Bioassay, Canine)
• Fu Z, Thorpe M, Hellman L rMCP-2, the Major Rat Mucosal Mast Cell Protease, an Analysis of Its Extended Cleavage Specificity and Its Potential Role in Regulating Intestinal Permeability by the Cleavage of Cell Adhesion and Junction Proteins. PLoS ONE, 2015-06-26;10(6):e0131720. 2015-06-26 [PMID: 26114959] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Cdh1
• Uniprot:
  • Q9R0T4()