Recombinant Mouse Follistatin 288 (FS-288) Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Follistatin 288 (FS-288) Protein, CF Summary

• Details of Functionality: Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED₅₀ for this effect is 0.1‑0.4 µg/mL in the presence of 7.5 ng/mL rhActivin A.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Follistatin protein
  Gly30-Asn317, with an N-terminal Met
• Accession #: P47931
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fst
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Follistatin 288 (FS-288) Protein, CF

• follistatin isoform FST317
• Follistatin
• FS
• FSActivin-binding protein
• FST

Background

Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high-affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS-288) and 315 aa residues (FS-315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS-315 composed of 300 aa residues. FS-288 binds with high-affinity to cell-surface heparan sulfate proteoglycans whereas FS-315 binds with low-affinity. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Overexpression of FS can also cause reproductive defects in transgenic mice.

1. Iemura, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9337.
2. Guo, Q. (1998) Mol. Endocrinol. 12:96.
3. Hashimoto, O. et al. (1997) J. Biol. Chem. 272:13835.

Publications for Follistatin (769-FS/CF)(10)

Publications using 769-FS/CF

• T Kurosawa, M Goto, N Kaji, S Aikiyo, T Mihara, M Ikemoto-Ue, M Toyoda, N Kanazawa, T Nakazawa, M Hori, A Uezumi Liver fibrosis-induced muscle atrophy is mediated by elevated levels of circulating TNF&amp;alpha Cell Death & Disease, 2021-01-07;12(1):11. 2021-01-07 [PMID: 33414474] (Bioassay, Mouse)
• A Ikeda, T Yamamoto, J Mineshiba, S Takashiba Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells Sci Rep, 2020-11-17;10(1):19959. 2020-11-17 [PMID: 33203957] (Cell Culture, Mouse)
• MA Cortez, F Masrorpour, C Ivan, J Zhang, A Younes, Y Lu, MR Estecio, HB Barsoumian, H Menon, MDS Caetano, R Ramapriyan, JE Schoenhals, X Wang, F Skoulidis, MD Wasley, G Calin, P Hwu, JW Welsh Bone morphogenetic protein 7 promotes resistance to immunotherapy Nat Commun, 2020-09-24;11(1):4840. 2020-09-24 [PMID: 32973129] (Bioassay, Mouse)
• Wang Z, Niu W, Wang Y, Teng Z, Wen J, Xia G, Wang C Follistatin288 Regulates Germ Cell Cyst Breakdown and Primordial Follicle Assembly in the Mouse Ovary. PLoS ONE, 2015-06-15;10(6):e0129643. 2015-06-15 [PMID: 26076381] (Bioassay, Mouse)
• Chen C, Murray P, Jiang T, Plikus M, Chang Y, Lee O, Widelitz R, Chuong C Regenerative hair waves in aging mice and extra-follicular modulators follistatin, dkk1, and sfrp4. J Invest Dermatol, 2014-03-11;134(8):2086-96. 2014-03-11 [PMID: 24618599] (In Vivo, Mouse)
• Silla Z, Naidoo J, Kidson S, Sommer P Signals from the lens and Foxc1 regulate the expression of key genes during the onset of corneal endothelial development. Exp Cell Res, 2014-01-25;322(2):381-8. 2014-01-25 [PMID: 24472616] (Bioassay, Mouse)
• Braga M, Reddy S, Vergnes L, Pervin S, Grijalva V, Stout D, David J, Li X, Tomasian V, Reid C, Norris K, Devaskar S, Reue K, Singh R Follistatin promotes adipocyte differentiation, browning, and energy metabolism. J Lipid Res, 2014-01-17;55(3):375-84. 2014-01-17 [PMID: 24443561] (Bioassay, Mouse)
• Roelandt P, Pauwelyn KA, Sancho-Bru P Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells. PLoS ONE, 2010-08-11;5(8):e12101. 2010-08-11 [PMID: 20711405] (Cell Culture, Rat)
• Sun Y, Ge Y, Drnevich J, Zhao Y, Band M, Chen J Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis. J. Cell Biol., 2010-06-21;189(7):1157-69. 2010-06-21 [PMID: 20566686] (Bioassay, Mouse)
• Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 2006-08-30;133(19):3787-96. 2006-08-30 [PMID: 16943279] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Fst
• Uniprot:
  • P47931()