Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein, CF


Recombinant Human/Mouse/Rat GDF-8/Myostatin (Catalog # 788-G8/CF) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 2-10 ng/mL.
1 μg/lane of Recombinant Human/Mouse/Rat GDF-8/Myostatin was resolved withSDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by silver staining, showing the main bands at 12 kDa and 24 kDa, more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications Bioactivity

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Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein, CF Summary

Details of Functionality
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 2-10 ng/mL.
Mouse myeloma cell line, NS0-derived GDF-8/Myostatin protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
12 kDa, reducing conditions
24 kDa, non-reducing conditions
Read Publications using
788-G8/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein, CF

  • GDF8
  • GDF-8
  • GDF8growth differentiation factor 8
  • growth/differentiation factor 8
  • MSTN
  • Myostatin


Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF‑ beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Sharma, M. et al. (1999) J. Cell Physiol. 180:1.
  3. McPherron, A.C. et al. (1997) Nature 387:83.
  4. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
  5. Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.

Publications for GDF-8/Myostatin (788-G8/CF)(40)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Porcine.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Control, ELISA (Standard), ELISA Capture, ELISA Developmet, In Vivo, Surface Plasmon Resonance.

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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using 788-G8/CF Applications Species
R Mishra, R Jha, B Mishra, YS Kim Maternal immunization against myostatin suppresses post-hatch chicken growth PLoS ONE, 2022-10-06;17(10):e0275753. 2022-10-06 [PMID: 36201511] (Bioassay, Human) Bioassay Human
DK Sung, H Kim, SE Park, J Lee, JA Kim, YC Park, HB Jeon, JW Chang, J Lee A New Method of Myostatin Inhibition in Mice via Oral Administration of Lactobacillus casei Expressing Modified Myostatin Protein, BLS-M22 International Journal of Molecular Sciences, 2022-08-13;23(16):. 2022-08-13 [PMID: 36012334] (ELISA Capture, Mouse) ELISA Capture Mouse
A Hagg, S Kharoud, G Goodchild, CA Goodman, JL Chen, RE Thomson, H Qian, P Gregorevic, CA Harrison, KL Walton TMEPAI/PMEPA1 Is a Positive Regulator of Skeletal Muscle Mass Front Physiol, 2020-11-04;11(0):560225. 2020-11-04 [PMID: 33250771] (Bioassay, Human) Bioassay Human
YC Chang, HW Liu, YC Chan, SH Hu, MY Liu, SJ Chang The green tea polyphenol epigallocatechin-3-gallate attenuates age-associated muscle loss via regulation of miR-486-5p and myostatin Arch. Biochem. Biophys., 2020-07-22;692(0):108511. 2020-07-22 [PMID: 32710883] (Bioassay, Mouse) Bioassay Mouse
EAC Goossens, MR de Vries, JW Jukema, PHA Quax, AY Nossent Myostatin Inhibits Vascular Smooth Muscle Cell Proliferation and Local 14q32 microRNA Expression, But Not Systemic Inflammation or Restenosis Int J Mol Sci, 2020-05-15;21(10):. 2020-05-15 [PMID: 32429150] (Cell Culture, In Vivo, Mouse) Cell Culture, In Vivo Mouse
M Kodaka, F Mao, K Arimoto-Ma, M Kitamura, X Xu, Z Yang, K Nakagawa, J Maruyama, K Ishii, C Akazawa, T Oyaizu, N Yamamoto, M Ishigami-Y, N Tsuemoto, S Ito, H Kagechika, H Nishina, Y Hata Characterization of a novel compound that promotes myogenesis via�Akt and transcriptional co-activator with PDZ-binding motif (TAZ) in mouse C2C12 cells PLoS ONE, 2020-04-08;15(4):e0231265. 2020-04-08 [PMID: 32267872] (Bioassay, Human) Bioassay Human
KB Dagbay, E Treece, FC Streich, JW Jackson, RR Faucette, A Nikiforov, SC Lin, CJ Boston, SB Nicholls, AD Capili, GJ Carven Structural basis of specific inhibition of extracellular activation of pro- or latent myostatin by the monoclonal antibody SRK-015 J. Biol. Chem., 2020-02-19;295(16):5404-5418. 2020-02-19 [PMID: 32075906] (Human) Human
XH Liu, JP Pan, WA Bauman, CP Cardozo AdipoRon prevents myostatin-induced upregulation of fatty acid synthesis and downregulation of insulin activity in a mouse hepatocyte line Physiol Rep, 2019-08-01;7(13):e14152. 2019-08-01 [PMID: 31250564] (Cell Culture, Bioassay, Mouse) Cell Culture, Bioassay Mouse
Q Jin, C Qiao, J Li, B Xiao, J Li, X Xiao A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice Skelet Muscle, 2019-05-27;9(1):16. 2019-05-27 [PMID: 31133057] (Bioassay, Human) Bioassay Human
JH Kim, JH Kim, LA Sutikno, SB Lee, DH Jin, YK Hong, YS Kim, HJ Jin Identification of the minimum region of flatfish myostatin propeptide (Pep45-65) for myostatin inhibition and its potential to enhance muscle growth and performance in animals PLoS ONE, 2019-04-18;14(4):e0215298. 2019-04-18 [PMID: 30998775] (Bioassay, Human) Bioassay Human
Show All 40 Publications.

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Gene Symbol MSTN