Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein, CF

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1 μg/lane of Recombinant Human/Mouse/Rat GDF-8/Myostatin was resolved withSDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by silver staining, showing the main bands at 12 kDa and 24 kDa, ...read more
Recombinant Human/Mouse/Rat GDF-8/Myostatin (Catalog # 788-G8/CF) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 2-10 ng/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein, CF Summary

Details of Functionality
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 2-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived GDF-8/Myostatin protein
Asp268-Ser376
Accession #
N-terminal Sequence
Asp268
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
MSTN
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing conditions
Publications
Read Publications using
788-G8/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein, CF

  • GDF8
  • GDF-8
  • GDF8growth differentiation factor 8
  • growth/differentiation factor 8
  • MSLHP
  • MSTN
  • Myostatin

Background

Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF‑ beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Sharma, M. et al. (1999) J. Cell Physiol. 180:1.
  3. McPherron, A.C. et al. (1997) Nature 387:83.
  4. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
  5. Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.

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Publications for GDF-8/Myostatin (788-G8/CF)(39)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, N/A, Porcine.

We have publications tested in 10 applications: Binding Assay, Bioassay, Cell Culture, Control, ELISA (Standard), ELISA Developmet, Immunocapture LCMS Standard, In Vivo, Not listed, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(1)
Bioassay
(25)
Cell Culture
(2)
Control
(1)
ELISA (Standard)
(1)
ELISA Developmet
(1)
Immunocapture LCMS Standard
(1)
In Vivo
(3)
Not listed
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(18)
Mouse
(16)
Rat
(1)
Chicken
(1)
N/A
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using 788-G8/CF Applications Species
A Hagg, S Kharoud, G Goodchild, CA Goodman, JL Chen, RE Thomson, H Qian, P Gregorevic, CA Harrison, KL Walton TMEPAI/PMEPA1 Is a Positive Regulator of Skeletal Muscle Mass Front Physiol, 2020;11(0):560225. 2020 [PMID: 33250771] (Bioassay, Human) Bioassay Human
YC Chang, HW Liu, YC Chan, SH Hu, MY Liu, SJ Chang The green tea polyphenol epigallocatechin-3-gallate attenuates age-associated muscle loss via regulation of miR-486-5p and myostatin Arch. Biochem. Biophys., 2020;692(0):108511. 2020 [PMID: 32710883] (Not listed, Mouse) Not listed Mouse
EAC Goossens, MR de Vries, JW Jukema, PHA Quax, AY Nossent Myostatin Inhibits Vascular Smooth Muscle Cell Proliferation and Local 14q32 microRNA Expression, But Not Systemic Inflammation or Restenosis Int J Mol Sci, 2020;21(10):. 2020 [PMID: 32429150] (In Vivo, Mouse) In Vivo Mouse
M Kodaka, F Mao, K Arimoto-Ma, M Kitamura, X Xu, Z Yang, K Nakagawa, J Maruyama, K Ishii, C Akazawa, T Oyaizu, N Yamamoto, M Ishigami-Y, N Tsuemoto, S Ito, H Kagechika, H Nishina, Y Hata Characterization of a novel compound that promotes myogenesis via�Akt and transcriptional co-activator with PDZ-binding motif (TAZ) in mouse C2C12 cells PLoS ONE, 2020;15(4):e0231265. 2020 [PMID: 32267872] (Bioassay, Human) Bioassay Human
KB Dagbay, E Treece, FC Streich, JW Jackson, RR Faucette, A Nikiforov, SC Lin, CJ Boston, SB Nicholls, AD Capili, GJ Carven Structural basis of specific inhibition of extracellular activation of pro- or latent myostatin by the monoclonal antibody SRK-015 J. Biol. Chem., 2020;295(16):5404-5418. 2020 [PMID: 32075906] (Human) Human
XH Liu, JP Pan, WA Bauman, CP Cardozo AdipoRon prevents myostatin-induced upregulation of fatty acid synthesis and downregulation of insulin activity in a mouse hepatocyte line Physiol Rep, 2019;7(13):e14152. 2019 [PMID: 31250564] (Bioassay, Mouse) Bioassay Mouse
XH Liu, JP Pan, WA Bauman, CP Cardozo AdipoRon prevents myostatin-induced upregulation of fatty acid synthesis and downregulation of insulin activity in a mouse hepatocyte line Physiol Rep, 2019;7(13):e14152. 2019 [PMID: 31250564] (Cell Culture, Mouse) Cell Culture Mouse
Q Jin, C Qiao, J Li, B Xiao, J Li, X Xiao A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice Skelet Muscle, 2019;9(1):16. 2019 [PMID: 31133057] (Bioassay, Human) Bioassay Human
JH Kim, JH Kim, LA Sutikno, SB Lee, DH Jin, YK Hong, YS Kim, HJ Jin Identification of the minimum region of flatfish myostatin propeptide (Pep45-65) for myostatin inhibition and its potential to enhance muscle growth and performance in animals PLoS ONE, 2019;14(4):e0215298. 2019 [PMID: 30998775] (Bioassay, Human) Bioassay Human
JD Yoon, SU Hwang, M Kim, G Lee, Y Jeon, SH Hyun GDF8 enhances SOX2 expression and blastocyst total cell number in porcine IVF embryo development Theriogenology, 2019;129(0):70-76. 2019 [PMID: 30825707] (Bioassay, Porcine) Bioassay Porcine
Show All 39 Publications.

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Bioinformatics

Gene Symbol MSTN
Uniprot