Recombinant Mouse Chordin Protein, CF

Images

 
There are currently no images for Chordin (758-CN/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse Chordin Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 2‑6 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP) and 1 µg/mL of recombinant mouse TSG.
Source
Mouse myeloma cell line, NS0-derived mouse Chordin protein
Mouse Chordin
(Thr27-Ser948)
Accession # Q9Z0E2
IEGR GGGSGGGSGGGS 10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Thr27
Protein/Peptide Type
Recombinant Proteins
Gene
Chrd
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
101.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-115 kDa, reducing conditions
Publications
Read Publications using
758-CN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Chordin Protein, CF

  • Chordin
  • CHRD
  • MGC133038
  • Sog

Background

Chordin is a secreted glycoprotein that regulates dorsoventral patterning during gastrulation. Chordin functions as a bone morphogenetic protein (BMP) antagonist that blocks their ventralizing activity by binding to the BMPs and inhibiting their interaction with their receptors. Mouse Chordin cDNA encodes a 948 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide. Chordin contains four internal cysteine-rich repeats (CRs) that are conserved in the spacing of their ten cysteine residues. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding. These conserved CRs are present in an expanding family of secreted molecules that antagonize BMP signaling. Xolloid (an extracellular zinc metalloproteinase) can cleave chordin at two specific sites resulting in chordin fragments with lower BMP-affinity. Cleavage of the chordin/BMP complex can reverse the BMP antagonist activity of chordin. Mouse chordin is expressed at high levels in 7 day postcoitum mouse embryos. Chordin expression is also detected in multiple fetal and adult tissues, most notably liver and cerebellum, suggesting additional roles for chordin in organogenesis and homeostasis.

  1. De Robertis, E.M. and Y. Sasai (1996) Nature 380:37.
  2. Larrain, J. et al. (2000) Development 127:821.
  3. Coffinier, C. et al. (2001) Mech. Dev. 100:119.

Customers Who Viewed This Item Also Viewed...

355-BM/CF
Species: Hu, Mu, Rt
AF1927
Species: Hu
Applications: WB, IP, ICC
NB100-56883
Species: Hu
Applications: IHC, IHC-P
46780002
Species: Hu
Applications: ELISA
355-BM/CF
Species: Hu, Mu, Rt
AF2299
Species: Mu
Applications: WB, IHC
AF1075
Species: Hu
Applications: WB
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF756
Species: Mu
Applications: WB, IHC
NBP1-87508
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-20486
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-47790
Species: Hu
Applications: WB, Flow, IHC, IHC-P

Publications for Chordin (758-CN/CF)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 4 applications: Binding Assay, Bioassay, In Vivo, Receptor Binding Assay.


Filter By Application
Binding Assay
(1)
Bioassay
(6)
In Vivo
(1)
Receptor Binding Assay
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(4)
Rat
(1)
Chicken
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using 758-CN/CF Applications Species
Zakin L, Chang EY, Plouhinec JL, De Robertis EM Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos. Dev. Biol., 2010;347(1):204-15. 2010 [PMID: 20807528] (Bioassay, Mouse) Bioassay Mouse
Jablonska B, Aguirre A, Raymond M, Szabo G, Kitabatake Y, Sailor KA, Ming GL, Song H, Gallo V Chordin-induced lineage plasticity of adult SVZ neuroblasts after demyelination. Nat. Neurosci., 2010;13(5):541-50. 2010 [PMID: 20418875] (In Vivo, Mouse) In Vivo Mouse
Hayashi K, Surani MA Self-renewing epiblast stem cells exhibit continual delineation of germ cells with epigenetic reprogramming in vitro. Development, 2009;136(21):3549-56. 2009 [PMID: 19793888] (Bioassay, Mouse) Bioassay Mouse
Kelley R, Ren R, Pi X, Wu Y, Moreno I, Willis M, Moser M, Ross M, Podkowa M, Attisano L, Patterson C A concentration-dependent endocytic trap and sink mechanism converts Bmper from an activator to an inhibitor of Bmp signaling. J. Cell Biol., 2009;184(4):597-609. 2009 [PMID: 19221194] (Bioassay, Mouse) Bioassay Mouse
Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning. Dev. Cell, 2008;15(2):248-60. 2008 [PMID: 18694564] (Binding Assay, Human) Binding Assay Human
Boswell BA, Lein PJ, Musil LS Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells. Mol. Biol. Cell, 2008;19(6):2631-41. 2008 [PMID: 18400943] (Bioassay, Chicken) Bioassay Chicken
McLean AB, D&amp;amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 2007;25(1):29-38. 2007 [PMID: 17204604] (Bioassay, Human) Bioassay Human
Maeshima A, Vaughn DA, Choi Y, Nigam SK Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct. Dev. Biol., 2006;295(2):473-85. 2006 [PMID: 16643884] (Bioassay, Rat) Bioassay Rat
Reversade B, De Robertis EM Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field. Cell, 2005;123(6):1147-60. 2005 [PMID: 16360041] (Receptor Binding Assay, Human) Receptor Binding Assay Human

Reviews for Chordin (758-CN/CF) (0)

There are no reviews for Chordin (758-CN/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Chordin (758-CN/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Chordin Products

Bioinformatics Tool for Chordin (758-CN/CF)

Discover related pathways, diseases and genes to Chordin (758-CN/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Chordin (758-CN/CF)

Discover more about diseases related to Chordin (758-CN/CF).
 

Pathways for Chordin (758-CN/CF)

View related products by pathway.

PTMs for Chordin (758-CN/CF)

Learn more about PTMs related to Chordin (758-CN/CF).

Blogs on Chordin

There are no specific blogs for Chordin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Chordin Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Chrd
Entrez
Uniprot