Recombinant Mouse Chordin Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Chordin Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 2‑6 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP) and 1 µg/mL of recombinant mouse TSG.
Mouse myeloma cell line, NS0-derived mouse Chordin protein
Mouse Chordin
Accession # Q9Z0E2
N-terminus C-terminus
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
101.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
100-115 kDa, reducing conditions
Read Publications using
758-CN/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Chordin Protein, CF

  • Chordin
  • CHRD
  • MGC133038
  • Sog


Chordin is a secreted glycoprotein that regulates dorsoventral patterning during gastrulation. Chordin functions as a bone morphogenetic protein (BMP) antagonist that blocks their ventralizing activity by binding to the BMPs and inhibiting their interaction with their receptors. Mouse Chordin cDNA encodes a 948 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide. Chordin contains four internal cysteine-rich repeats (CRs) that are conserved in the spacing of their ten cysteine residues. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding. These conserved CRs are present in an expanding family of secreted molecules that antagonize BMP signaling. Xolloid (an extracellular zinc metalloproteinase) can cleave chordin at two specific sites resulting in chordin fragments with lower BMP-affinity. Cleavage of the chordin/BMP complex can reverse the BMP antagonist activity of chordin. Mouse chordin is expressed at high levels in 7 day postcoitum mouse embryos. Chordin expression is also detected in multiple fetal and adult tissues, most notably liver and cerebellum, suggesting additional roles for chordin in organogenesis and homeostasis.

  1. De Robertis, E.M. and Y. Sasai (1996) Nature 380:37.
  2. Larrain, J. et al. (2000) Development 127:821.
  3. Coffinier, C. et al. (2001) Mech. Dev. 100:119.

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Publications for Chordin (758-CN/CF)(11)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Xenopus.

We have publications tested in 4 applications: Binding Assay, Bioassay, In Vivo, Receptor Binding Assay.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 758-CN/CF Applications Species
CN Wang, SN Gong, W Guan, JL Wang, TT Gao, Y Wang, F Sun, B Jiang Hippocampal overexpression of chordin protects against the chronic social defeat stress-induced depressive-like effects in mice Brain Res. Bull., 2020-02-24;158(0):31-39. 2020-02-24 [PMID: 32105677] (In Vivo, Mouse) In Vivo Mouse
L Sieron, M Lesiak, I Schisler, Z Drzazga, A Fertala, AL Sieron Functional and structural studies of tolloid-like 1 (TLL1) mutants associated with atrial-septal defect 6 (ASD6) Biosci. Rep., 2019-01-08;0(0):. 2019-01-08 [PMID: 30538173] (Bioassay, Mouse) Bioassay Mouse
Zakin L, Chang EY, Plouhinec JL, De Robertis EM Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos. Dev. Biol., 2010-08-31;347(1):204-15. 2010-08-31 [PMID: 20807528] (Bioassay, Mouse) Bioassay Mouse
Jablonska B, Aguirre A, Raymond M, Szabo G, Kitabatake Y, Sailor KA, Ming GL, Song H, Gallo V Chordin-induced lineage plasticity of adult SVZ neuroblasts after demyelination. Nat. Neurosci., 2010-04-25;13(5):541-50. 2010-04-25 [PMID: 20418875] (In Vivo, Mouse) In Vivo Mouse
Hayashi K, Surani MA Self-renewing epiblast stem cells exhibit continual delineation of germ cells with epigenetic reprogramming in vitro. Development, 2009-09-30;136(21):3549-56. 2009-09-30 [PMID: 19793888] (Bioassay, Mouse) Bioassay Mouse
Kelley R, Ren R, Pi X, Wu Y, Moreno I, Willis M, Moser M, Ross M, Podkowa M, Attisano L, Patterson C A concentration-dependent endocytic trap and sink mechanism converts Bmper from an activator to an inhibitor of Bmp signaling. J. Cell Biol., 2009-02-16;184(4):597-609. 2009-02-16 [PMID: 19221194] (Bioassay, Mouse) Bioassay Mouse
Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning. Dev. Cell, 2008-08-01;15(2):248-60. 2008-08-01 [PMID: 18694564] (Binding Assay, Human) Binding Assay Human
Boswell BA, Lein PJ, Musil LS Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells. Mol. Biol. Cell, 2008-04-09;19(6):2631-41. 2008-04-09 [PMID: 18400943] (Bioassay, Chicken) Bioassay Chicken
McLean AB, D&amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 2007-01-01;25(1):29-38. 2007-01-01 [PMID: 17204604] (Bioassay, Human) Bioassay Human
Maeshima A, Vaughn DA, Choi Y, Nigam SK Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct. Dev. Biol., 2006-04-27;295(2):473-85. 2006-04-27 [PMID: 16643884] (Bioassay, Rat) Bioassay Rat
Show All 11 Publications.

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Gene Symbol Chrd