Human FLRG Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human FLRG Quantikine ELISA Kit Summary

• Background: The Quantikine Human FLRG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FLRG in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human FLRG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FLRG showed linear curves that were parallel to the standard curves obtai...ned using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FLRG.
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• Specificity: Natural and recombinant human FLRG
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FSTL3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human FLRG Quantikine ELISA Kit

• FLRG
• FLRGFSRP
• follistatin-like 3 (secreted glycoprotein)
• Follistatin-like 3
• Follistatin-like protein 3
• Follistatin-related gene protein
• follistatin-related protein 3
• FSTL3

Background

Follistatin-related gene protein (FLRG) has been shown to bind and inhibit the TGF-beta family ligands including activin, BMP-2, -6, -7, and GDF-8. FLRG is expressed most highly in heart, lung, kidney, and placenta, and is upregulated by TGF-beta and Activin via Smad proteins.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Follistatin-related Gene Protein/FLRG/Fstl3 (DFLRG0)(8)

Publications using DFLRG0

• Duan, W;Shi, R;Yang, F;Zhou, Z;Wang, L;Huang, Z;Zang, S; FSTL3 partially mediates the association of increased nonalcoholic fatty liver disease fibrosis risk with acute myocardial infarction in patients with type 2 diabetes mellitus Cardiovascular diabetology 2023-10-30 [PMID: 37904173] (Human)
• J Luther, AG Vannier, EA Schaefer, RP Goodman The circulating proteomic signature of alcohol-associated liver disease JCI Insight, 2022-07-22;7(14):. 2022-07-22 [PMID: 35866482] (Human)
• M McAleavy, Q Zhang, PJ Ehmann, J Xu, MF Wipperman, D Ajithdoss, L Pan, M Wakai, R Simonson, A Gadi, A Oyejide, SC Hamon, A Boyapati, LG Morton, T Shavlakadz, CA Kyratsous, DJ Glass The Activin/FLRG pathway associates with poor COVID-19 outcomes in hospitalized patients Molecular and Cellular Biology, 2021-11-01;0(0):MCB0046721. 2021-11-01 [PMID: 34723652] (Human)
• S Gong, F Gaccioli, J Dopierala, U Sovio, E Cook, PJ Volders, L Martens, PDW Kirk, S Richardson, GCS Smith, DS Charnock-J The RNA landscape of the human placenta in health and disease Nature Communications, 2021-05-11;12(1):2639. 2021-05-11 [PMID: 33976128] (Human)
• LV Barrios-Si, M Parnell, ZB Shinwari, GA Chaudhary, T Xenofontos, A van Bekhov, S McArthur, BT Elliott Activin subfamily peptides predict chronological age in humans Physiol Rep, 2018-09-01;6(17):e13823. 2018-09-01 [PMID: 30178598] (Human)
• LM Rice, JC Mantero, EA Stratton, R Warburton, K Roberts, N Hill, RW Simms, R Domsic, HW Farber, R Layfatis Serum biomarker for diagnostic evaluation of pulmonary arterial hypertension in systemic sclerosis Arthritis Res. Ther., 2018-08-16;20(1):185. 2018-08-16 [PMID: 30115106] (Human)
• AT Amaraseker, B Assadi-Kha, S Liu, M Black, G Dymmott, NM Rogers, AL Sverdlov, JD Horowitz, DTM Ngo Vitamin D supplementation lowers thrombospondin-1 levels and blood pressure in healthy adults PLoS ONE, 2017-05-10;12(5):e0174435. 2017-05-10 [PMID: 28489857] (Human)
• Naf S, Escote X, Ballesteros M, Yanez R, Simon-Muela I, Gil P, Albaiges G, Vendrell J, Megia A Serum activin A and follistatin levels in gestational diabetes and the association of the Activin A-Follistatin system with anthropometric parameters in offspring. PLoS ONE, 2014-04-24;9(4):e92175. 2014-04-24 [PMID: 24763182] (Human)

FAQs for Follistatin-related Gene Protein/FLRG/Fstl3 (DFLRG0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FSTL3