Noggin Antibody [Unconjugated]

Noggin was detected in immersion fixed frozen sections of embryonic mouse cardiac tissue (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Noggin was detected in immersion fixed PC-3 human prostate cancer cell line using Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Myo/Nog cells contain ink in human tattooed skin.Tissue sections of tattooed skin were double labeled with the G8 mAb and antibodies to Noggin (Nog) (A-D), MyoD (F-I) or alpha -SMA (K-N). The colors of the secondary antibodies are indicated in the photographs. Nuclei were stained with Hoechst dye (blue). Images were produced with the epifluorescence (A-E, J and O) and confocal microscopes (F-I and K-N) with 60x lenses. Overlap of green and red fluorescence appears yellow in merged images (C, D, H, I, M and N). Fluorescent photomicrographs were merged with the corresponding DIC image to visualize the ink (black in D, I and N). Some double labeled cells appeared to contain ink (arrows in D, I and N). All ink laden G8+ cells were alpha -SMA+ (N). Smooth muscle cells of blood vessels also contained alpha -SMA (K). Minimal to no background fluorescence was visible after staining with the anti-goat (E), anti-IgM and anti-IgG (I), and anti-rabbit (O) secondary antibodies only. Bar = 28 μM in E and 5.6 μM in A-D and G-O. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32833999), licensed under a CC-BY license. Not internally tested by R&D Systems.

Myo/Nog cells contain ink in human tattooed skin.Tissue sections of tattooed skin were double labeled with the G8 mAb and antibodies to Noggin (Nog) (A-D), MyoD (F-I) or alpha -SMA (K-N). The colors of the secondary antibodies are indicated in the photographs. Nuclei were stained with Hoechst dye (blue). Images were produced with the epifluorescence (A-E, J and O) and confocal microscopes (F-I and K-N) with 60x lenses. Overlap of green and red fluorescence appears yellow in merged images (C, D, H, I, M and N). Fluorescent photomicrographs were merged with the corresponding DIC image to visualize the ink (black in D, I and N). Some double labeled cells appeared to contain ink (arrows in D, I and N). All ink laden G8+ cells were alpha -SMA+ (N). Smooth muscle cells of blood vessels also contained alpha -SMA (K). Minimal to no background fluorescence was visible after staining with the anti-goat (E), anti-IgM and anti-IgG (I), and anti-rabbit (O) secondary antibodies only. Bar = 28 μM in E and 5.6 μM in A-D and G-O. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32833999), licensed under a CC-BY license. Not internally tested by R&D Systems.

Myo/Nog cells contain ink in human tattooed skin.Tissue sections of tattooed skin were double labeled with the G8 mAb and antibodies to Noggin (Nog) (A-D), MyoD (F-I) or alpha -SMA (K-N). The colors of the secondary antibodies are indicated in the photographs. Nuclei were stained with Hoechst dye (blue). Images were produced with the epifluorescence (A-E, J and O) and confocal microscopes (F-I and K-N) with 60x lenses. Overlap of green and red fluorescence appears yellow in merged images (C, D, H, I, M and N). Fluorescent photomicrographs were merged with the corresponding DIC image to visualize the ink (black in D, I and N). Some double labeled cells appeared to contain ink (arrows in D, I and N). All ink laden G8+ cells were alpha -SMA+ (N). Smooth muscle cells of blood vessels also contained alpha -SMA (K). Minimal to no background fluorescence was visible after staining with the anti-goat (E), anti-IgM and anti-IgG (I), and anti-rabbit (O) secondary antibodies only. Bar = 28 μM in E and 5.6 μM in A-D and G-O. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32833999), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Noggin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Noggin
  Leu20-Cys232
  Accession # P97466
• Specificity: Detects mouse Noggin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Noggin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NOG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Noggin Antibody [Unconjugated]

• NOG
• Noggin
• SYM1
• symphalangism 1 (proximal)
• synostoses (multiple) syndrome 1
• SYNS1
• SYNS1A

Background

Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian Noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse Noggin shares 99% and 83% aa sequence identity with human and Xenopus Noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, Noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.

1. Smith, W.C. and R.M. Harland (1992) Cell 70:829.
2. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
3. Brunet, L.J. et al. (1998) Science 280:1455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Noggin Antibody (AF719)(19)

Publications using AF719

• M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins bioRxiv : the preprint server for biology, 2023-04-04;0(0):. 2023-04-04 [PMID: 37066397] (IHC, Mouse)
• M Crispin, J Gerhart, A Heffer, M Martin, F Abdalla, A Bravo-Nuev, NJ Philp, AE Kuriyan, M George-Wei Myo/Nog Cells Give Rise to Myofibroblasts During Epiretinal Membrane Formation in a Mouse Model of Proliferative Vitreoretinopathy Investigative Ophthalmology & Visual Science, 2023-02-01;64(2):1. 2023-02-01 [PMID: 36723927] (IHC, Mouse)
• Ying-Wen Wang, Wen-Yu Lin, Fang-Ju Wu, Ching-Wei Luo Unveiling the transcriptomic landscape and the potential antagonist feedback mechanisms of TGF-beta superfamily signaling module in bone and osteoporosis Cell Communication and Signaling 2022-11-28 [PMID: 36443839]
• S Joseph-Pau, N Morrison, M Braccia, A Payne, L Gugerty, J Mostoller, P Lecker, EJ Tsai, J Kim, M Martin, R Brahmbhatt, G Gorski, J Gerhart, M George-Wei, J Stone, S Purushothu, A Bravo-Nuev Acute Response and Neuroprotective Role of Myo/Nog Cells Assessed in a Rat Model of Focal Brain Injury Frontiers in Neuroscience, 2021-12-07;15(0):780707. 2021-12-07 [PMID: 34949984] (IHC, Rat)
• J Geuder, LE Wange, A Janjic, J Radmer, P Janssen, JW Bagnoli, S Müller, A Kaul, M Ohnuki, W Enard A non-invasive method to generate induced pluripotent stem cells from primate urine Scientific Reports, 2021-02-10;11(1):3516. 2021-02-10 [PMID: 33568724] (ICC, Human)
• J Gerhart, L Gugerty, P Lecker, F Abdalla, M Martin, O Gerhart, C Gerhart, K Johal, J Bernstein, J Spikes, K Mathers, A Bravo-Nuev, M George-Wei Myo/Nog cells are nonprofessional phagocytes PLoS ONE, 2020-08-24;15(8):e0235898. 2020-08-24 [PMID: 32833999] (IHC, Human, Mouse)
• J Gerhart, J Bowers, L Gugerty, C Gerhart, M Martin, F Abdalla, A Bravo-Nuev, JT Sullivan, R Rimkunas, A Albertus, L Casta, L Getts, R Getts, M George-Wei Brain-specific angiogenesis inhibitor 1 is expressed in the Myo/Nog cell lineage PLoS ONE, 2020-07-02;15(7):e0234792. 7/2/2020 [PMID: 32614850]
• Eric Engelbrecht, Michel V Levesque, Liqun He, Michael Vanlandewijck, Anja Nitzsche, Hira Niazi et al. Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta eLife 2020-02-24 [PMID: 32091396]
• AM Blázquez-M, M Jumabay, P Rajbhandar, T Sallam, Y Guo, J Yao, L Vergnes, K Reue, L Zhang, Y Yao, AM Fogelman, P Tontonoz, AJ Lusis, X Wu, KI Boström Noggin depletion in adipocytes promotes obesity in mice Mol Metab, 2019-04-10;0(0):. 2019-04-10 [PMID: 31027994] (Functional Assay, IHC, Mouse)
• J Gerhart, K Behling, M Paessler, L Milton, G Bramblett, D Garcia, M Pitts, R Hurtt, M Crawford, R Lackman, D Nguyen, J Infanti, P FitzGerald, M George-Wei Rhabdomyosarcoma and Wilms tumors contain a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins PLoS ONE, 2019-04-11;14(4):e0214758. 2019-04-11 [PMID: 30973903] (ICC, IHC, IHC-P, Human)
• J Gerhart, C Withers, C Gerhart, L Werner, N Mamalis, A Bravo-Nuev, V Scheinfeld, P FitzGerald, R Getts, M George-Wei Myo/Nog cells are present in the ciliary processes, on the zonule of Zinn and posterior capsule of the lens following cataract surgery Exp. Eye Res., 2018-03-17;171(0):101-105. 2018-03-17 [PMID: 29559302] (IHC-P, Human, Mouse, Rabbit)
• Jacquelyn Gerhart, Marvin Greenbaum, Lou Casta, Anthony Clemente, Keith Mathers, Robert Getts et al. Antibody-Conjugated, DNA-Based Nanocarriers Intercalated with Doxorubicin Eliminate Myofibroblasts in Explants of Human Lens Tissue Journal of Pharmacology and Experimental Therapeutics 2017-04-01 [PMID: 28096456]
• Ayan Ray, Pratik Narendra Pratap Singh, Michael L. Sohaskey, Richard M. Harland, Amitabha Bandyopadhyay Precise spatial restriction of BMP signaling is essential for articular cartilage differentiation Development 2015-03-15 [PMID: 25758226]
• Gerhart J, Greenbaum M, Scheinfeld V, Fitzgerald P, Crawford M, Bravo-Nuevo A, Pitts M, George-Weinstein M Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens. PLoS ONE, 2014-04-15;9(4):e95262. 2014-04-15 [PMID: 24736495] (ICC, IHC-Fr, Human)
• Li T, Longobardi L, Myers T, Temple J, Chandler R, Ozkan H, Contaldo C, Spagnoli A Joint TGF-beta type II receptor-expressing cells: ontogeny and characterization as joint progenitors. Stem Cells Dev, 2013-02-15;22(9):1342-59. 2013-02-15 [PMID: 23231014] (IHC, Mouse)
• Jacquelyn Gerhart, Victoria L. Scheinfeld, Tara Milito, Jessica Pfautz, Christine Neely, Dakota Fisher-Vance et al. Myo/Nog cell regulation of bone morphogenetic protein signaling in the blastocyst is essential for normal morphogenesis and striated muscle lineage specification Developmental Biology 2011-11-01 [PMID: 21884693]
• Ono Y, Calhabeu F, Morgan JE BMP signalling permits population expansion by preventing premature myogenic differentiation in muscle satellite cells. Cell Death Differ., 2010-08-06;18(2):222-34. 2010-08-06 [PMID: 20689554] (ICC, Mouse)
• Gerhart J, Pfautz J, Neely C, Elder J, DuPrey K, Menko AS, Knudsen K, George-Weinstein M Noggin producing, MyoD-positive cells are crucial for eye development. Dev. Biol., 2009-09-22;336(1):30-41. 2009-09-22 [PMID: 19778533] (IHC, Chicken)
• Gerhart J, Elder J, Neely C, Schure J, Kvist T, Knudsen K, George-Weinstein M MyoD-positive epiblast cells regulate skeletal muscle differentiation in the embryo. J. Cell Biol., 2006-10-23;175(2):283-92. 2006-10-23 [PMID: 17060497] (IHC-P, Chicken)

Review for Noggin Antibody (AF719) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 01/09/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24042462
• Species: Mouse

Bioinformatics

• Gene Symbol: NOG
• Uniprot:
  • P97466()