Noggin Antibody

Noggin was detected in immersion fixed frozen sections of embryonic mouse cardiac tissue (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Noggin was detected in immersion fixed PC‑3 human prostate cancer cell line using Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Noggin Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Noggin
  Leu20-Cys232
  Accession # P97466
• Specificity: Detects mouse Noggin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Noggin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NOG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Noggin Antibody

• NOG
• Noggin
• SYM1
• symphalangism 1 (proximal)
• synostoses (multiple) syndrome 1
• SYNS1
• SYNS1A

Background

Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian Noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse Noggin shares 99% and 83% aa sequence identity with human and Xenopus Noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, Noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.

1. Smith, W.C. and R.M. Harland (1992) Cell 70:829.
2. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
3. Brunet, L.J. et al. (1998) Science 280:1455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Noggin Antibody (AF719)(8)

Publications using AF719

• AM Blázquez-M, M Jumabay, P Rajbhandar, T Sallam, Y Guo, J Yao, L Vergnes, K Reue, L Zhang, Y Yao, AM Fogelman, P Tontonoz, AJ Lusis, X Wu, KI Boström Noggin depletion in adipocytes promotes obesity in mice Mol Metab, 2019;0(0):. 2019 [PMID: 31027994] (Functional Assay, Mouse)
• J Gerhart, K Behling, M Paessler, L Milton, G Bramblett, D Garcia, M Pitts, R Hurtt, M Crawford, R Lackman, D Nguyen, J Infanti, P FitzGerald, M George-Wei Rhabdomyosarcoma and Wilms tumors contain a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins PLoS ONE, 2019;14(4):e0214758. 2019 [PMID: 30973903] (ICC, Human)
• J Gerhart, C Withers, C Gerhart, L Werner, N Mamalis, A Bravo-Nuev, V Scheinfeld, P FitzGerald, R Getts, M George-Wei Myo/Nog cells are present in the ciliary processes, on the zonule of Zinn and posterior capsule of the lens following cataract surgery Exp. Eye Res., 2018;171(0):101-105. 2018 [PMID: 29559302] (IHC-P, Human)
• Gerhart J, Greenbaum M, Scheinfeld V, Fitzgerald P, Crawford M, Bravo-Nuevo A, Pitts M, George-Weinstein M Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens. PLoS ONE, 2014;9(4):e95262. 2014 [PMID: 24736495] (ICC, Human)
• Li T, Longobardi L, Myers T, Temple J, Chandler R, Ozkan H, Contaldo C, Spagnoli A Joint TGF-beta type II receptor-expressing cells: ontogeny and characterization as joint progenitors. Stem Cells Dev, 2013;22(9):1342-59. 2013 [PMID: 23231014]
• Ono Y, Calhabeu F, Morgan JE BMP signalling permits population expansion by preventing premature myogenic differentiation in muscle satellite cells. Cell Death Differ., 2011;18(2):222-34. 2011 [PMID: 20689554] (ICC, Mouse)
• Gerhart J, Pfautz J, Neely C, Elder J, DuPrey K, Menko AS, Knudsen K, George-Weinstein M Noggin producing, MyoD-positive cells are crucial for eye development. Dev. Biol., 2009;336(1):30-41. 2009 [PMID: 19778533] (IHC, Chicken)
• Gerhart J, Elder J, Neely C, Schure J, Kvist T, Knudsen K, George-Weinstein M MyoD-positive epiblast cells regulate skeletal muscle differentiation in the embryo. J. Cell Biol., 2006;175(2):283-92. 2006 [PMID: 17060497] (IHC-P, Chicken)

Review for Noggin Antibody (AF719) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 01/09/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24042462
• Species: Mouse

Blocking

• Blocking Details: See PMID 24042462

Details

• Detection Notes: See PMID 24042462

Comments

• Comments: Published: See PMID 24042462Independent review from antibodyresource.comRequired extended incubation at 4 degrees but produced a very clean, strong signal

Bioinformatics

• Gene Symbol: NOG
• Uniprot:
  • P97466()