Human Follistatin Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Follistatin Quantikine ELISA Kit Summary

• Background: The Quantikine Human Follistatin Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human Follistatin (FS288, FS300, and FS315) in follicular fluid, cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human Follistatin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human Follis...tatin. Results obtained using natural Follistatin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural Follistatin.
  Show More
• Specificity: Natural and recombinant human Follistatin
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FST

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Follistatin Quantikine ELISA Kit

• follistatin isoform FST317
• Follistatin
• FS
• FSActivin-binding protein
• FST

Background

Follistatin (FST) is a secreted glycoprotein that binds and neutralizes several TGF-beta superfamily proteins including Activin, BMP-6, BMP-7, and GDF-8/Myostatin. It also binds Angiogenin and regulates hematopoietic stem cell adhesion to Fibronectin. Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3. Of three follistatin isoforms, the full-length mature follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative forms. The acidic tail is missing in the splice variant FST288, which shows highest affinity, and partial in the proteolytically produced FST303, which shows intermediate affinity. Genetic deletion of follistatin in mice, or expression of only the FST288 form, is lethal perinatally due to defects of lung, skin and musculoskeletal system. Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Follistatin (DFN00)(36)

Publications using DFN00

• Nawrocka-Millward, S;Biegus, J;Fudim, M;Guzik, M;Iwanek, G;Ponikowski, P;Zymli?ski, R; The role of urine chloride in acute heart failure Scientific reports 2024-06-18 [PMID: 38890417] (Human)
• Baccouche, B;Lietuvninkas, L;Kazlauskas, A; Activin A Limits VEGF-Induced Permeability via VE-PTP International journal of molecular sciences 2023-05-12 [PMID: 37240047] (Human)
• R Tao, O Stöhr, C Wang, W Qiu, KD Copps, MF White Hepatic follistatin increases basal metabolic rate and attenuates diet-induced obesity during hepatic insulin resistance Molecular Metabolism, 2023-03-10;71(0):101703. 2023-03-10 [PMID: 36906067] (Mouse)
• MM Richter, MS Svane, VB Kristianse, JJ Holst, S Madsbad, KN Bojsen-Møl Postprandial secretion of follistatin after gastric bypass surgery and sleeve gastrectomy Peptides, 2023-02-24;163(0):170978. 2023-02-24 [PMID: 36842630] (Human)
• M Baumgartne, J Lischka, A Schanzer, C de Gier, NK Walleczek, S Greber-Pla, M Zeyda Plasma Myostatin Increases with Age in Male Youth and Negatively Correlates with Vitamin D in Severe Pediatric Obesity Nutrients, 2022-05-20;14(10):. 2022-05-20 [PMID: 35631274] (Human)
• CA Perry, GP Van Guilde, TA Butterick Decreased myostatin in response to a controlled DASH diet is associated with improved body composition and cardiometabolic biomarkers in older adults: results from a controlled-feeding diet intervention study BMC nutrition, 2022-03-15;8(1):24. 2022-03-15 [PMID: 35287731] (Human)
• M Amasene, C Cadenas-Sa, I Echeverria, B Sanz, C Alonso, I Tobalina, J Irazusta, I Labayen, A Besga Effects of Resistance Training Intervention along with Leucine-Enriched Whey Protein Supplementation on Sarcopenia and Frailty in Post-Hospitalized Older Adults: Preliminary Findings of a Randomized Controlled Trial Journal of Clinical Medicine, 2021-12-24;11(1):. 2021-12-24 [PMID: 35011838] (Human)
• S Nawrocka-M, J Biegus, M Hurkacz, M Guzik, M Rosiek-Bie, EA Jankowska, P Ponikowski, R Zymli?ski Differences in the Biomarker Profile of De Novo Acute Heart Failure versus Decompensation of Chronic Heart Failure Biomolecules, 2021-11-16;11(11):. 2021-11-16 [PMID: 34827701] (Human)
• C Wu, Y Borné, R Gao, M López Rodr, WC Roell, JM Wilson, A Regmi, C Luan, DM Aly, A Peter, J Machann, H Staiger, A Fritsche, AL Birkenfeld, R Tao, R Wagner, M Canouil, MG Hong, JM Schwenk, E Ahlqvist, MU Kaikkonen, P Nilsson, AC Shore, F Khan, A Natali, O Melander, M Orho-Melan, J Nilsson, HU Häring, E Renström, CB Wollheim, G Engström, J Weng, ER Pearson, PW Franks, MF White, KL Duffin, AA Vaag, M Laakso, N Stefan, L Groop, Y De Marinis Elevated circulating follistatin associates with an increased risk of type 2 diabetes Nature Communications, 2021-11-10;12(1):6486. 2021-11-10 [PMID: 34759311] (Human)
• Y Du, C Xu, H Shi, X Jiang, W Tang, X Wu, M Chen, H Li, X Zhang, Q Cheng Serum concentrations of oxytocin, DHEA and follistatin are associated with osteoporosis or sarcopenia in community-dwelling postmenopausal women BMC geriatrics, 2021-10-12;21(1):542. 2021-10-12 [PMID: 34641817] (Human)
• Z He, Y Tian, PL Valenzuela, C Huang, J Zhao, P Hong, Z He, S Yin, A Lucia Myokine/Adipokine Response to "Aerobic" Exercise: Is It Just a Matter of Exercise Load? Front Physiol, 2019-05-29;10(0):691. 2019-05-29 [PMID: 31191366] (Human)
• S Sarcar, W Tulalamba, MY Rincon, J Tipanee, HQ Pham, H Evens, D Boon, E Samara-Kuk, M Keyaerts, M Loperfido, E Berardi, S Jarmin, P In't Veld, G Dickson, T Lahoutte, M Sampaolesi, P De Bleser, T VandenDrie, MK Chuah Next-generation muscle-directed gene therapy by in silico vector design Nat Commun, 2019-01-30;10(1):492. 2019-01-30 [PMID: 30700722] (Mouse)
• Z He, Y Tian, PL Valenzuela, C Huang, J Zhao, P Hong, Z He, S Yin, A Lucia Myokine Response to High-Intensity Interval vs. Resistance Exercise: An Individual Approach Front Physiol, 2018-12-03;9(0):1735. 2018-12-03 [PMID: 30559681] (Human)
• E Fife, J Kostka, ? Kroc, A Guligowska, M Pig?owska, B So?tysik, A Kaufman-Sz, K Fabianowsk, T Kostka Relationship of muscle function to circulating myostatin, follistatin and GDF11 in older women and men BMC Geriatr, 2018-08-30;18(1):200. 2018-08-30 [PMID: 30165829] (Human)
• R Tao, C Wang, O Stöhr, W Qiu, Y Hu, J Miao, XC Dong, S Leng, M Stefater, N Stylopoulo, L Lin, KD Copps, MF White Inactivating hepatic follistatin alleviates hyperglycemia Nat. Med., 2018-06-04;0(0):. 2018-06-04 [PMID: 29867232] (Mouse)
• J Ma, C Pichavant, H du Bois, M Bhakta, MP Calos DNA-Mediated Gene Therapy in a Mouse Model of Limb Girdle Muscular Dystrophy 2B Mol Ther Methods Clin Dev, 2017-10-24;7(0):123-131. 2017-10-24 [PMID: 29159199] (Mouse)
• BT Elliott, P Herbert, N Sculthorpe, FM Grace, D Stratton, LD Hayes Lifelong exercise, but not short-term high-intensity interval training, increases GDF11, a marker of successful aging: a�preliminary investigation Physiol Rep, 2017-07-11;5(13):. 2017-07-11 [PMID: 28701523] (Human)
• K Ikeda, A Ito, R Imada, M Sato, Y Kawabe, M Kamihira In vitro drug testing based on contractile activity of C2C12 cells in an epigenetic drug model Sci Rep, 2017-03-16;7(0):44570. 2017-03-16 [PMID: 28300163] (Human)
• Stelios Loukides Activin A and follistatin in patients with asthma. Does severity make the difference? Respirology, 2016-11-02;0(0):. 2016-11-02 [PMID: 27807906] (Human)
• Wilson C, Ottewell P, Coleman R, Holen I The differential anti-tumour effects of zoledronic acid in breast cancer - evidence for a role of the activin signaling pathway. BMC Cancer, 2015-02-14;15(0):55. 2015-02-14 [PMID: 25884855] (Human)
• Naf S, Escote X, Ballesteros M, Yanez R, Simon-Muela I, Gil P, Albaiges G, Vendrell J, Megia A Serum activin A and follistatin levels in gestational diabetes and the association of the Activin A-Follistatin system with anthropometric parameters in offspring. PLoS ONE, 2014-04-24;9(4):e92175. 2014-04-24 [PMID: 24763182] (Human)
• Yaden B, Croy J, Wang Y, Wilson J, Datta-Mannan A, Shetler P, Milner A, Bryant H, Andrews J, Dai G, Krishnan V Follistatin: a novel therapeutic for the improvement of muscle regeneration. J Pharmacol Exp Ther, 2014-03-13;349(2):355-71. 2014-03-13 [PMID: 24627466] (Mouse)
• Leddy H, McNulty A, Lee S, Rothfusz N, Gloss B, Kirby M, Hutson M, Cohn D, Guilak F, Liedtke W Follistatin in chondrocytes: the link between TRPV4 channelopathies and skeletal malformations. FASEB J, 2014-02-27;28(6):2525-37. 2014-02-27 [PMID: 24577120] (Human)
• Ferguson B, Gao X, Zelazowski M, Lee J, Jeter C, Abba M, Aldaz C The cancer gene WWOX behaves as an inhibitor of SMAD3 transcriptional activity via direct binding. BMC Cancer, 2013-12-11;13(0):593. 2013-12-11 [PMID: 24330518] (Human)
• Chen Y, Wu Y, Yang W, Wang S, Lee C, Chou N, Hsu R, Lin Y, Lin M, Ho Y, Chen M Relationship between bone mineral density and serum osteoprotegerin in patients with chronic heart failure. PLoS ONE, 2012-08-30;7(8):e44242. 2012-08-30 [PMID: 22957004] (Human)
• Karve TM, Preet A, Sneed R BRCA1 regulates follistatin function in ovarian cancer and human ovarian surface epithelial cells. PLoS ONE, 2012-06-01;7(6):e37697. 2012-06-01 [PMID: 22685544] (Human)
• Jeppesen JV, Nielsen ME, Kristensen SG, Yding Andersen C Concentration of activin A and follistatin in follicular fluid from human small antral follicles associated to gene expression of the corresponding granulosa cells. Mol. Cell. Endocrinol., 2011-08-09;356(1):48-54. 2011-08-09 [PMID: 21846490] (Human)
• Hansen J, Brandt C, Nielsen AR, Hojman P, Whitham M, Febbraio MA, Pedersen BK, Plomgaard P Exercise induces a marked increase in plasma follistatin: evidence that follistatin is a contraction-induced hepatokine. Endocrinology, 2010-11-10;152(1):164-71. 2010-11-10 [PMID: 21068158] (Human, Mouse)
• Sun Y, Ge Y, Drnevich J, Zhao Y, Band M, Chen J Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis. J. Cell Biol., 2010-06-21;189(7):1157-69. 2010-06-21 [PMID: 20566686] (Mouse)
• Foley JW, Bercury SD, Finn P Evaluation of systemic follistatin as an adjuvant to stimulate muscle repair and improve motor function in Pompe mice. Mol. Ther., 2010-06-15;18(9):1584-91. 2010-06-15 [PMID: 20551907] (Human)
• Weigert J, Neumeier M, Wanninger J, Schober F, Sporrer D, Weber M, Schramm A, Wurm S, Stogbauer F, Filarsky M, Schaffler A, Aslanidis C, Scholmerich J, Buechler C Adiponectin upregulates monocytic activin A but systemic levels are not altered in obesity or type 2 diabetes. Cytokine, 2009-01-06;45(2):86-91. 2009-01-06 [PMID: 19128983] (Human)
• Eijken M, Swagemakers S, Koedam M, Steenbergen C, Derkx P, Uitterlinden AG, van der Spek PJ, Visser JA, de Jong FH, Pols HA, van Leeuwen JP The activin A-follistatin system: potent regulator of human extracellular matrix mineralization. FASEB J., 2007-04-20;21(11):2949-60. 2007-04-20 [PMID: 17449718] (Human)
• Miller TM, Kim SH, Yamanaka K, Hester M, Umapathi P, Arnson H, Rizo L, Mendell JR, Gage FH, Cleveland DW, Kaspar BK Gene transfer demonstrates that muscle is not a primary target for non-cell-autonomous toxicity in familial amyotrophic lateral sclerosis. Proc. Natl. Acad. Sci. U.S.A., 2006-12-12;103(51):19546-51. 2006-12-12 [PMID: 17164329] (Mouse)
• Tardif G, Hum D, Pelletier JP, Boileau C, Ranger P, Martel-Pelletier J Differential gene expression and regulation of the bone morphogenetic protein antagonists follistatin and gremlin in normal and osteoarthritic human chondrocytes and synovial fibroblasts. Arthritis Rheum., 2004-08-01;50(8):2521-30. 2004-08-01 [PMID: 15334466] (Human)
• Abe Y, Abe T, Aida Y, Hara Y, Maeda K Follistatin restricts bone morphogenetic protein (BMP)-2 action on the differentiation of osteoblasts in fetal rat mandibular cells. J. Bone Miner. Res., 2004-05-03;19(8):1302-7. 2004-05-03 [PMID: 15231018] (Rat)
• Leal AM, Takabe K, Wang L, Donaldson CJ, MacConell LA, Bilezikjian LM, Verma IM, Vale W Effect of adenovirus-mediated overexpression of follistatin and extracellular domain of activin receptor type II on gonadotropin secretion in vitro and in vivo. Endocrinology, 2002-03-01;143(3):964-9. 2002-03-01 [PMID: 11861519] (Transgenic Rat)

FAQs for Follistatin (DFN00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FST