GDF-5/BMP-14 Antibody [Unconjugated]

GDF‑5 was detected in immersion fixed frozen sections of mouse embyro (12 d.p.c.) using 15 µg/mL Goat Anti-Mouse GDF‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse GDF‑5/BMP‑14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF‑5/BMP‑14 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GDF-5/BMP-14 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse GDF-5
  Ala376-Arg495
  Accession # P43027
• Specificity: Detects mouse GDF-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) GDF-6 is observed, and less than 5% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF‑8, rmGDF‑9, recombinant human GDF-11, and rhGDF-15 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GDF5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-5/BMP-14 Antibody [Unconjugated]

• BMP14
• BMP-14
• Cartilage-derived morphogenetic protein 1
• cartilage-derived morphogenetic protein-1
• CDMP-1
• CDMP1LAP4
• GDF5
• GDF-5
• growth differentiation factor 5
• growth/differentiation factor 5
• OS5
• radotermin
• SYNS2

Background

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region, and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6, and GDF-7, which share 80-86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

1. Storm, E.E. et al. (1994) Nature 368:639.
2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
3. Francis-West, P.H. et al. (1999) Development 126:1035.
4. Massague, J. et al. (2000) Genes and Dev. 14:627.
5. Settle, S.H. Jr. et al. (2003) Dev. Biol. 254:116.
6. Inada, M. et al. (1996) BBRC 222:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GDF-5/BMP-14 Antibody (AF853)(4)

Publications using AF853

• Bethanie Imogen Ayerst GDF5 Mediated Enhancement of Chondrocyte Phenotype is Inhibited by Heparin; Implication for the use of Heparin in the Clinic and in Tissue Engineering Applications Tissue Eng Part A, 2016;0(0):. 2016 [PMID: 27899064] (Western Blot, Mouse)
• Li T, Longobardi L, Myers T, Temple J, Chandler R, Ozkan H, Contaldo C, Spagnoli A Joint TGF-beta type II receptor-expressing cells: ontogeny and characterization as joint progenitors. Stem Cells Dev, 2013;22(9):1342-59. 2013 [PMID: 23231014] (IHC, Mouse)
• Zaidi SH, Huang Q, Momen A, Riazi A, Husain M Growth differentiation factor 5 regulates cardiac repair after myocardial infarction. J. Am. Coll. Cardiol., 2010;55(2):135-43. 2010 [PMID: 20117381] (IHC-P, Mouse)
• Basile P, Dadali T, Jacobson J, Hasslund S, Ulrich-Vinther M, Soballe K, Nishio Y, Drissi MH, Langstein HN, Mitten DJ, O'Keefe RJ, Schwarz EM, Awad HA Freeze-dried tendon allografts as tissue-engineering scaffolds for Gdf5 gene delivery. Mol. Ther., 2008;16(3):466-73. 2008 [PMID: 18180771] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: GDF5
• Uniprot:
  • P43027()