Recombinant Human Noggin Protein, CF


Recombinant Human Noggin (Catalog # 6057-NG/CF) inhibits BMP-4 induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The activity is approximately 30-fold greater than the top competitor's more
BG01V human embryonic stem cells were cultured in Mouse Embryonic Fibroblast Conditioned Media supplemented with FGF basic (5 ng/mL). Stem cells were driven into early cells of the neuroectoderm using a 3 day incubation more

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Recombinant Human Noggin Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.02-0.16 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).
Mouse myeloma cell line, NS0-derived human Noggin protein
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
23 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
30-33 kDa, reducing conditions
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6057-NG/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Noggin Protein, CF

  • NOG
  • Noggin
  • SYM1
  • symphalangism 1 (proximal)
  • synostoses (multiple) syndrome 1
  • SYNS1
  • SYNS1A


Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N-terminal acidic region, a central basic heparin‑binding segment and a C-terminal cysteine-knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4). During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons (6, 9 - 13). Noggin also appears to maintain adult stem cell populations in-vivo, for example, maintaining neural stem cells within the hippocampus (13).

  1. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  2. Groppe, J. et al. (2002) Nature 420:636.
  3. Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089.
  4. Brunet, L. J. et al. (1998) Science 280:1455.
  5. McMahon, J. A. et al. (1998) Genes Dev. 12:1438.
  6. Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24.
  7. Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607.
  8. Gong, Y. et al. (1999) Nat. Genet. 21:302.
  9. Xu, R.-H. et al. (2005) Nat. Methods 2:185.
  10. Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934.
  11. Chaturvedi, G. et al. (2009) Cell Prolif. 42:425.
  12. Chiba, S. et al. (2008) Stem Cells 26:2810.
  13. Bonaguidi, M.A. et al. (2008) J. Neurosci. 28:9194.

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Publications for Noggin (6057-NG/CF)(161)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Chicken, Equine, N/A, Porcine, Primate, Xenopus, Zebrafish.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Tissue Culture.

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Showing Publications 1 - 10 of 161. Show All 161 Publications.
Publications using 6057-NG/CF Applications Species
JM Suárez-Riv, CJ Pastor-Mal, A Romero-Gon, D Gómez-Fern, S Povea-Cabe, M Álvarez-Có, I Villalón-G, M Talaverón-, A Suárez-Car, M Munuera-Ca, JA Sánchez-Al Pterostilbene in Combination With Mitochondrial Cofactors Improve Mitochondrial Function in Cellular Models of Mitochondrial Diseases Frontiers in Pharmacology, 2022;13(0):862085. 2022 [PMID: 35370630] (Bioassay, Human) Bioassay Human
S Kim, S Min, YS Choi, SH Jo, JH Jung, K Han, J Kim, S An, YW Ji, YG Kim, SW Cho Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids Nature Communications, 2022;13(1):1692. 2022 [PMID: 35354790] (Bioassay, Human) Bioassay Human
J Zhou, H He, JJ Zhang, X Liu, W Yao, C Li, T Xu, SY Yin, DY Wu, CL Dou, Q Li, J Xiang, WJ Xiong, LY Wang, JM Tang, Z Xue, X Zhang, YL Miao ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation Autophagy, 2022;0(0):. 2022 [PMID: 35311460] (Bioassay, Human) Bioassay Human
K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids Stem Cell Reports, 2022;0(0):. 2022 [PMID: 35245440] (Bioassay, Human) Bioassay Human
K Zhao, Y Shi, J Yu, L Yu, A Mael, Y Li, A Kolton, T Joyce, J Odorico, PO Berggren, SN Yang Intracameral Microimaging of Maturation of Human iPSC Derivatives into Islet Endocrine Cells Cell Transplantation, 2022;31(0):9636897211066. 2022 [PMID: 35156411] (Bioassay, Human) Bioassay Human
S Vachenc, J Gobbo, SE Moujarrebe, I Desmoulins, M Gilabert, M Beau-Falle, E Mitry, N Girard, A Bertaut, N Dusetti, JL Iovanna, R Yousfi, F Pierrat, R Bruno, A Cueff, R Boidot, P Genne OncoSNIPE� Study Protocol, a study of molecular profiles associated with development of resistance in solid cancer patients BMC Cancer, 2022;22(1):41. 2022 [PMID: 34991520] (Bioassay, Human) Bioassay Human
N Yucer, R Ahdoot, MJ Workman, AH Laperle, MS Recouvreux, K Kurowski, DJ Naboulsi, V Liang, Y Qu, JT Plummer, SA Gayther, S Orsulic, BY Karlan, CN Svendsen Human iPSC-derived fallopian tube organoids with BRCA1 mutation recapitulate early-stage carcinogenesis Cell Reports, 2021;37(13):110146. 2021 [PMID: 34965417] (Bioassay, Human) Bioassay Human
A Bruzelius, S Kidnapilla, J Drouin-Oue, T Stoker, RA Barker, D Rylander O Reprogramming Human Adult Fibroblasts into GABAergic Interneurons Cells, 2021;10(12):. 2021 [PMID: 34943958] (Bioassay, Human) Bioassay Human
Y Cheng, TR Hall, X Xu, I Yung, D Souza, J Zheng, F Schiele, M Hoffmann, ML Mbow, JP Garnett, J Li Targeting uPA-uPAR interaction to improve intestinal epithelial barrier integrity in inflammatory bowel disease EBioMedicine, 2021;75(0):103758. 2021 [PMID: 34933179] (Bioassay, Human) Bioassay Human
R Sharma, A George, M Nimmagadda, D Ortolan, BS Karla, Z Qureshy, D Bose, R Dejene, G Liang, Q Wan, J Chang, BS Jha, O Memon, KJ Miyagishim, A Rising, M Lal, E Hanson, R King, MM Campos, M Ferrer, J Amaral, D McGaughey, K Bharti Epithelial phenotype restoring drugs suppress macular degeneration phenotypes in an iPSC model Nature Communications, 2021;12(1):7293. 2021 [PMID: 34911940] (Bioassay, Human) Bioassay Human
Show All 161 Publications.

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Gene Symbol NOG